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A novel plant protein-disulfide isomerase participates in resistance response against the TYLCV in tomato

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Abstract

Main conclusion

Overexpression or silencing of the SlPDI could increase plants resistance or sensitivity to TYLCV through enhancing or reducing the plant’s antioxidant capacity.

Abstract

Tomato yellow leaf curl virus (TYLCV), a plant virus that could infect a variety of crops, is particularly destructive to tomato growth. Protein disulfide isomerase (PDI) is a member of the thioredoxin (Trx) superfamily, is capable of catalyzing the formation and heterogeneity of protein disulfide bonds and inhibiting the aggregation of misfolded proteins. Studies have shown that PDI plays important roles in plant response to abiotic stress, there is no research report on the function of PDI in response to biotic stress, especially TYLCV infection. Here, we identified a tomato PDI gene, SlPDI, was involved in regulating tomato plants resistance to TYLCV. Subcellular localization results showed that SlPDI was located at the endoplasmic reticulum (ER), and its location remained unchanged after infection with TYLCV virus. Overexpression or silencing of SlPDI could increase plants resistance or sensitivity to TYLCV. Transgenic plants that overexpressing SlPDI exhibit enhanced antioxidant activity evidenced by lower hydrogen peroxide (H2O2) level and higher activity of superoxide dismutase (SOD) and peroxidase (POD) in comparison with WT plants, after infected by TYLCV. Moreover, the SlPDI-silencing plants showed opposite results. The promoter analyzes result showed that SlPDI was involved in response to salicylic acid (SA), and our experimental results also showed that the expression level of SlPDI was induced by SA. Taken together, our results indicated that SlPDI could regulate plant resistance to TYLCV through enhancing the protein folding function of ER and promoting the synthesis and conformation of antioxidant-related proteins.

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Abbreviations

DAB:

Diaminobenzidine;

ER:

Endoplasmic reticulum;

H2O2 :

Hydrogen peroxide;

MDA:

Malondialdehyde;

ORF:

Open reading frame;

PDI:

Protein disulfide isomerase;

POD:

Peroxidase;

RT-qPCR:

Quantitative real-time PCR;

ROS:

Reactive oxygen species;

SA:

Salicylic acid;

SOD:

Superoxide dismutase;

TYLCV:

Tomato yellow leaf curl virus;

VIGS:

Virus-induced gene silencing;

WT:

Wild type

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Acknowledgements

The research was supported by New Century Excellent Talents in University (NCET-11-0670), Jiangsu Natural Science Foundation (BK20130027), and Priority Academic Program Development of Jiangsu Higher Education Institutions Project (PAPD).

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China

    Tong Li, Ya-Hui Wang, Ying Huang, Jie-Xia Liu, Zhi-Sheng Xu & Ai-Sheng Xiong

  2. Collaborative Innovation Center for Improving Quality and Increase Profits of Protected Vegetables in Shanxi, Shanxi Agricultural University, Taigu, China

    Guo-Ming Xing, Sheng Sun & Sen Li

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  1. Tong Li
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  2. Ya-Hui Wang
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Correspondence to Ai-Sheng Xiong.

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Li, T., Wang, YH., Huang, Y. et al. A novel plant protein-disulfide isomerase participates in resistance response against the TYLCV in tomato. Planta 252, 25 (2020). https://doi.org/10.1007/s00425-020-03430-1

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