Main conclusion
In the wheat TCMS line YS3038, the anther development is inhibited from late uninucleate stage to the binucleate stage. The disruption of energy metabolism pathways by aberrant transcriptional regulation causes the male sterility under low temperatures.
Abstract
The utilization of thermosensitive male sterile (TMS) lines provides a basis for two-line breeding. Previous work, including morphological and cytological observations, has shown that the development process of the TMS line YS3038 is inhibited from the late uninucleate stage to the binucleate stage. Transcriptomics studies could now help to elucidate the overall expression of related genes in a specific reproductive process, revealing the metabolic network and its regulatory mechanism of the reproductive process from the transcription level. Considering the fertility characteristics of YS3038, three important stages for transcriptome analysis were determined to be the early uninucleate, late uninucleate and binucleate stages. The number of differentially expressed genes (DEGs) was found to be highest in the binucleate stage, and most were related to energy metabolism. Quantitative PCR analysis of selected genes related to energy metabolism revealed that their expression patterns were consistent with the sequencing results. Analysis of the fertility mechanism of YS3038 showed that although the tapetum of anthers was degraded in advance of the tetrad stage, the development of microspores did not result in obvious abnormalities until the binucleate stage, because the genes involved in energy metabolism pathways, including starch and sucrose metabolism (SSM), glycolysis, the tricarboxylic acid (TCA) cycle, oxidative phosphorylation, and respiration electron transport chain are differentially expressed under sterile and fertile conditions. Therefore, the pollen in YS3038 was sterile.
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Abbreviations
- CMS:
-
Cytoplasmic male sterility
- DEGs:
-
Differentially expressed genes
- SSM:
-
Starch and sucrose metabolism
- TCA:
-
Tricarboxylic acid cycle
- TCMS:
-
Thermosensitive cytoplasmic male sterility
- TMS:
-
Thermosensitive male sterile
- YSF:
-
YS3038 fertile line
- YSS:
-
YS3038 sterile line
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This study was supported by the National Key Research and Development Program of China (2016YFD0101602) and the “948” Program of the Ministry of Agriculture (2016-X58).
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Han, Y., Gao, Y., Zhao, Y. et al. Energy metabolism involved in fertility of the wheat TCMS line YS3038. Planta 250, 2159–2171 (2019). https://doi.org/10.1007/s00425-019-03281-5
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DOI: https://doi.org/10.1007/s00425-019-03281-5