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Ammonium-induced architectural and anatomical changes with altered suberin and lignin levels significantly change water and solute permeabilities of rice (Oryza sativa L.) roots

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Abstract

Main conclusion

Non-optimal ammonium levels significantly alter root architecture, anatomy and root permeabilities for water and nutrient ions. Higher ammonium levels induced strong apoplastic barriers whereas it was opposite for lower levels.

Application of nitrogen fertilizer increases crop productivity. However, non-optimal applications can have negative effects on plant growth and development. In this study, we investigated how different levels of ammonium (NH4 +) [low (30 or 100 μM) or optimum (300 μM) or high (1000 or 3000 μM)] affect physio-chemical properties of 1-month-old, hydroponically grown rice roots. Different NH4 + treatments markedly altered the root architecture and anatomy. Plants grown in low NH4 + had the longest roots with a weak deposition of suberised and lignified apoplastic barriers, and it was opposite for plants grown in high NH4 +. The relative expression levels of selected suberin and lignin biosynthesis candidate genes, determined using qRT-PCR, were lowest in the roots from low NH4 +, whereas, they were highest for those grown in high NH4 +. This was reflected by the suberin and lignin contents, and was significantly lower in roots from low NH4 + resulting in greater hydraulic conductivity (Lp r) and solute permeability (P sr) than roots from optimum NH4 +. In contrast, roots grown at high NH4 + had markedly greater suberin and lignin contents, which were reflected by strong barriers. These barriers significantly decreased the P sr of roots but failed to reduce the Lp r below those of roots grown in optimum NH4 +, which can be explained in terms of the physical properties of the molecules used and the size of pores in the apoplast. It is concluded that, in rice, non-optimal NH4 + levels differentially affected root properties including Lp r and P sr to successfully adapt to the changing root environment.

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Abbreviations

CBs:

Casparian bands

CC:

Central cylinder

EN:

Endodermis

EX:

Exodermis

Lp r :

Hydraulic conductivity

OPR:

Outer part of the root

Psr:

Solute permeability

SL:

Suberin lamellae

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Acknowledgments

We thank Thakshila de Zoysa for help with plant care and data collection. This research was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) for SJR and the German Research Foundation (DFG Grant SCHR 506/12-1) for LS.

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Authors and Affiliations

  1. Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada

    Kosala Ranathunge, Yong-Mei Bi & Steven J. Rothstein

  2. Department of Ecophysiology, Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115, Bonn, Germany

    Lukas Schreiber

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  1. Kosala Ranathunge
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Correspondence to Kosala Ranathunge.

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Ranathunge, K., Schreiber, L., Bi, YM. et al. Ammonium-induced architectural and anatomical changes with altered suberin and lignin levels significantly change water and solute permeabilities of rice (Oryza sativa L.) roots. Planta 243, 231–249 (2016). https://doi.org/10.1007/s00425-015-2406-1

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