Abstract
Plant-specific ethylene response factors (ERFs) play important roles in abiotic and biotic stress responses in plants. Using a transgenic approach, we identified two rice ERF genes, OsERF4a and OsERF10a, which conferred drought stress tolerance. In particular, OsERF4a contains a conserved ERF-associated amphiphilic repression (EAR) motif in its C-terminal region that has been shown to function as a transcriptional repression domain. Expression profiling of transgenic rice plants over-expressing OsERF4a using either a constitutively active or an ABA-inducible promoter identified 45 down-regulated and 79 up-regulated genes in common. The increased stress tolerance by over-expression of the EAR domain-containing protein OsERF4a could result from suppression of a repressor of the defense response. Expression of the putative silent information regulator 2 (Sir2) repressor protein was repressed, and expression of several stress-response genes were induced by OsERF4a over-expression. The Sir2 and 7 out of 9 genes that were down-regulated by OsERF4a over-expression were induced by high salinity and drought treatments in non-transgenic control plants. Genes that were down- and up-regulated by OsERF4a over-expression were highly biased toward chromosome 11. Rice chromosome 11 has several large clusters of disease-resistance and defense-response genes. Taken together, our results suggest that OsERF4a is a positive regulator of shoot growth and water-stress tolerance in rice during early growth stages. We propose that OsERF4a could work by suppressing a repressor of the defense responses and/or by controlling the expression of a large number of genes located on chromosome 11.
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Abbreviations
- ERF:
-
Ethylene response factor
- EAR:
-
ERF-associated amphiphilic repression
- TF:
-
Transcription factors
- DRE:
-
Dehydration-responsive elements
- Sir2:
-
Silent information regulator 2
- qRT-PCR:
-
Quantitative real-time PCR
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Acknowledgments
This work was supported by the Technology Development Program for Life Industry through the Korea Institute of Planning and Evaluation for Technology of Food, Agriculture, Forestry and Fisheries (Grant number 111076-5).
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425_2013_1880_MOESM1_ESM.ppt
Supplementary material 1. Fig. S1 Stress tolerance of transgenic plants over-expressing OsERFs during the vegetative stage. T1 transgenic plants and NT controls were grown in a greenhouse for four weeks. The plants were subjected to 7 days of drought stress followed by rehydration in a greenhouse. a Drought stress tolerance test of T1 transgenic plants. b Drought stress tolerance of T1 OsERF4a and OsERF10a over-expressing transgenic plants. W, well-watered; D, drought-stressed and rehydrated. Photographs were taken on the indicated days. Fig. S2 Genomic Southern blotting analyses of 101ERF4a (a), 105ERF4a (b) and 101ERF10a (c) plants. Ten micrograms of genomic DNA were double-digested with EcoRI and XhoI restriction enzymes, separated on a 0.8 % agarose gel, blotted onto a membrane, and hybridized with a 32P-labeled MAR probe. (PPT 1,409 kb) (PPT 1,409 kb)
425_2013_1880_MOESM2_ESM.xlsx
Supplementary material 2. Table S1 A list of the rice ERF transcription factor genes used for rice transformation. Table S2 Down- and up-regulated genes in 101ERF4a and 105ERF4a plants compared with NT controls. Table S3 Commonly up-regulated genes in 101ERF4a and 105ERF4a plants compared with NT controls. Table S4 The chromosomal distribution of down- and up-regulated genes in rice plants over-expressing OsERF4a. Table S5 Gene-specific primers used for RT-PCR and quantitative RT-PCR. (XLSX 50 kb) (XLSX 50 kb)
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Joo, J., Choi, H.J., Lee, Y.H. et al. A transcriptional repressor of the ERF family confers drought tolerance to rice and regulates genes preferentially located on chromosome 11. Planta 238, 155–170 (2013). https://doi.org/10.1007/s00425-013-1880-6
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DOI: https://doi.org/10.1007/s00425-013-1880-6