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Overexpression of the pepper antimicrobial protein CaAMP1 gene regulates the oxidative stress- and disease-related proteome in Arabidopsis

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Abstract

Proteomics facilitates our understanding of cellular processes and network functions in the plant defense response during abiotic and biotic stresses. Here, we demonstrate that the ectopic expression of the Capsicum annuum antimicrobial protein CaAMP1 gene in Arabidopsis thaliana confers enhanced tolerance to methyl viologen (MV)-induced oxidative stress, which is accompanied by lower levels of lipid peroxidation. Quantitative comparative proteome analyses using two-dimensional gel electrophoresis coupled with mass spectrometry identified some of the oxidative stress- and disease-related proteins that are differentially regulated by CaAMP1 overexpression in Arabidopsis leaves. Antioxidant- and defense-related proteins, such as 2-cys peroxiredoxin, l-ascorbate peroxidase, peroxiredoxin, glutathione S-transferase and copper homeostasis factor, were up-regulated in the CaAMP1 transgenic leaf tissues. In contrast, GSH-dependent dehydroascorbate reductase and WD-40 repeat family protein were down-regulated by CaAMP1 overexpression. In addition, CaAMP1 overexpression enhanced resistance to Pseudomonas syringae pv. tomato (Pst) DC3000 infection and also H2O2 accumulation in Arabidopsis. The identified antioxidant- and defense-related genes were differentially expressed during MV-induced oxidative stress and Pst DC3000 infection. Taken together, we conclude that CaAMP1 overexpression can regulate the differential expression of defense-related proteins in response to environmental stresses to maintain reactive oxygen species (ROS) homeostasis.

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Abbreviations

2-DE:

Two-dimensional electrophoresis

APX:

l-ascorbate peroxidase

CHF:

Copper homeostasis factor

DHAR:

GSH-dependent dehydroascorbate reductase

GRP:

Glycine-rich protein

GST:

Glutathione S-transferase

MV:

Methyl viologen

OX:

Overexpression

PAGE:

Polyacrylamide gel electrophoresis

PAMP:

Pathogen-associated molecular pattern

Prx:

Peroxiredoxin

Pst :

Pseudomonas syringae pv. tomato

ROS:

Reactive oxygen species

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Acknowledgments

This work was supported by Grants from the Next Generation BioGreen21 Program (Plant Molecular Breeding Center No. PJ008027 and No. PJ008222), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. School of Biological Science (BK21 program), Chung-Ang University, Seoul, 156-756, Republic of Korea

    Sung Chul Lee

  2. Laboratory of Molecular Plant Pathology, School of Life Sciences and Biotechnology, Korea University, Anam-dong Sungbuk-ku, Seoul, 136-713, Republic of Korea

    In Sun Hwang & Byung Kook Hwang

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  1. Sung Chul Lee
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  2. In Sun Hwang
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Correspondence to Byung Kook Hwang.

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The nucleotide sequence data reported here has been deposited in the GenBank database under the accession number AY548741.

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Lee, S.C., Hwang, I.S. & Hwang, B.K. Overexpression of the pepper antimicrobial protein CaAMP1 gene regulates the oxidative stress- and disease-related proteome in Arabidopsis . Planta 234, 1111–1125 (2011). https://doi.org/10.1007/s00425-011-1473-1

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