Abstract
We investigated the deposition of glucomannans (GMs) in differentiating earlywood tracheids of Cryptomeria japonica using immunocytochemical methods. GMs began to deposit at the corner of the cell wall at the early stages of S1 formation and showed uneven distribution in the cell wall during S1 formation. At the early stages of S2 formation, limited GM labeling was observed in the S2 layer, and then the labeling increased gradually. In mature tracheids, the boundary between the S1 and S2 layers and the innermost part of the cell wall showed stronger labeling than other parts of the cell wall. Deacetylation of GMs with mild alkali treatment led to a significant increase in GM labeling and a more uniform distribution of GMs in the cell wall than that observed before deacetylation, indicating that some GM epitopes may be masked by acetylation. However, the changes in GM labeling after deacetylation were not very pronounced until early stages of S2 formation, indicating that GMs deposited in the cell wall at early stages of cell-wall formation may contain fewer acetyl groups than those deposited at later stages. Additionally, the density of GM labeling increased in the cell wall in both specimens before and after GM deacetylation, even after cell-wall formation was complete. This finding suggests that some acetyl groups may be removed from GMs after cell-wall formation is complete as part one of the tracheid cell aging processes.
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Abbreviations
- CML:
-
Compound middle lamella
- GMs:
-
Glucomannans
- GXs:
-
Glucuronoxylans
- Mt08:
-
Mature tracheids grown in 2008
- Mt09:
-
Mature tracheids grown in 2009
- PW:
-
Primary cell wall
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Acknowledgments
Jong Sik Kim is grateful for the Research Fellowships for Young Scientists provided by the Japan Society for the Promotion of Science (JSPS).
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Kim, J.S., Awano, T., Yoshinaga, A. et al. Temporal and spatial immunolocalization of glucomannans in differentiating earlywood tracheid cell walls of Cryptomeria japonica . Planta 232, 545–554 (2010). https://doi.org/10.1007/s00425-010-1189-7
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DOI: https://doi.org/10.1007/s00425-010-1189-7