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Ectopic expression of pepper CaPF1 in potato enhances multiple stresses tolerance and delays initiation of in vitro tuberization

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Abstract

Ethylene-responsive factors (ERFs) are plant-specific transcription factors, many of which have been linked to plant defense responses. However, little is known about the functional significance of ERF genes in potato plants compared to the model plant species Arabidopsis. We show here that overexpression of CaPF1, an ERF/AP2-type pepper transcription factor gene, effectively increased tolerance to freezing, heat, heavy metal, and oxidative stress in potatoes. Interestingly, CaPF1 was involved in tuber formation in potato plants. The time course of microtuber formation was significantly retarded in potato plants that overexpressed CaPF1 compared with wild-type potato plants. Overall, the results of the present study indicate that the pepper transcription factor gene, CaPF1, is involved in promotion of multiple stress tolerance and retardation of in vitro tuberization in potato plants.

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Abbreviations

CaPF1 :

Capsicum annuum pathogen freezing tolerance protein 1

ABA:

Abscisic acid

CTAB:

Cetyltrimethylammonium bromide

MV:

Methyl viologen

NPTII :

Neomycin phosphotransferase II

NT:

Not transformed

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Acknowledgments

This research was supported by a grant from the Plant Diversity Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology of Korean government and KRIBB Research Initiative Program.

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Authors and Affiliations

  1. Plant Genome Research Center, KRIBB, 111 Gwahangno, Yuseong-gu, Daejeon, 305-806, South Korea

    Jung Won Youm, Jae Heung Jeon, Hyouk Joung & Hyun Soon Kim

  2. Department of Plant Sciences, Seoul National University, Seoul, 151-921, South Korea

    Doil Choi

  3. Plant Immunity Research Group, RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, 230-0045, Japan

    So Young Yi

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  1. Jung Won Youm
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  2. Jae Heung Jeon
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  4. So Young Yi
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  5. Hyouk Joung
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  6. Hyun Soon Kim
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Correspondence to Hyun Soon Kim.

Electronic supplementary material

As shown in Fig. 6

Fig6

I. Freezing tolerance of CaPF1 transgenic potato plants. Potato plants of NT (a), CaPF1 expressing line 11 (b), and CaPF1 expressing line 16 (c) were exposed to −3°C for 15 h and then returned to 4°C for 4 h. Photographs were taken 2 weeks after return to normal growth conditions. Experiments were repeated two times (n = 6 each)II. Determination of relative freezing tolerance (RFT). Potato (cv. Atlantic) non-transgenic (NT) control, transgenic lines, CaPF1 11, 13, 15, 16, 17, 18 were grown at 25°C followed by cold acclimation overnight at 4°C, and the freezing tolerance of leaves was measured using the electrolyte leakage test. Experiments were repeated three times (n = 10) (JPG 243 kb)

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Youm, J.W., Jeon, J.H., Choi, D. et al. Ectopic expression of pepper CaPF1 in potato enhances multiple stresses tolerance and delays initiation of in vitro tuberization. Planta 228, 701–708 (2008). https://doi.org/10.1007/s00425-008-0782-5

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