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Function of mitochondria during the transition of barley protoplasts from low light to high light

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Abstract

Mitochondrial contribution to photosynthetic metabolism during the transition from low light (25–100 μmol quanta m−2 s−1, limiting photosynthesis) to high light (500 μmol quanta m−2 s−1, saturating photosynthesis) was investigated in protoplasts from barley (Hordeum vulgare) leaves. After the light shift, photosynthetic oxygen evolution rate increased rapidly during the first 30–40 s and then declined up to 60–70 s after which the rate increased to a new steady-state after 80–110 s. Rapid fractionation of protoplasts was used to follow changes in sub-cellular distribution of key metabolites during the light shift and the activation state of chloroplastic NADP-dependent malate dehydrogenase (EC 1.1.1.82) was measured. Although oligomycin (an inhibitor of the mitochondrial ATP synthase) affected the metabolite content of protoplasts following the light shift, the first oxygen burst was not affected. However, the transition to the new steady-state was delayed. Rotenone (an inhibitor of mitochondrial complex I) had similar, but less pronounced effect as oligomycin. From the analysis of metabolite content and sub-cellular distribution we suggest that the decrease in oxygen evolution following the first oxygen burst is due to phosphate limitation in the chloroplast stroma. For the recovery the control protoplasts can utilize ATP supplied by mitochondrial oxidative phosphorylation to quickly overcome the limitation in stromal phosphate and to increase the content of Calvin cycle metabolites. The oligomycin-treated protoplasts were deficient in cytosolic ATP and thereby unable to support Calvin cycle operation. This resulted in a delayed capacity to adjust to a sudden increase in light intensity.

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Abbreviations

MDH:

Malate dehydrogenase

OAA:

Oxaloacetate

PGA:

3-Phosphoglyceric acid

RuBP:

Ribulose-1,5-bisphosphate

TP:

Triose phosphate

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Acknowledgements

Skillful technical assistance of Gunilla Malmberg is greatly acknowledged. This work was supported by grants from the Swedish Royal Academy and the Swedish Research Council.

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Author notes

  1. Abir U. Igamberdiev

    Present address: Department of Plant Science, University of Manitoba, R3T 2N2, Winnipeg, MB, Canada

  2. Tongyun Shen

    Present address: The National Institute for Working Life, Box 7654, 907 13, Umea, Sweden

Authors and Affiliations

  1. Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 901 87 , Umea, Sweden

    Abir U. Igamberdiev, Tongyun Shen & Per Gardeström

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  1. Abir U. Igamberdiev
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  2. Tongyun Shen
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  3. Per Gardeström
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Correspondence to Per Gardeström.

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Igamberdiev, A.U., Shen, T. & Gardeström, P. Function of mitochondria during the transition of barley protoplasts from low light to high light. Planta 224, 196–204 (2006). https://doi.org/10.1007/s00425-005-0198-4

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