Abstract
The floral organs are formed from floral meristem with a regular initiation pattern in angiosperm species. Flowers of naked seed rice (nsr) were characterized by the overdeveloped lemma and palea, the transformation of lodicules to palea-/lemma-like organs, the decreased number of stamens and occasionally extra pistils. Some nsr spikelets contained additional floral organs of four whorls and/or abnormal internal florets. The floral primordium of nsr spikelet is differentiated under an irregular pattern and an incomplete determination. And molecular analysis indicated that nsr was a novel homeotic mutation in OsMADS1, suggesting that OsMADS1 played a distinct role in regulating the differentiation pattern of floral primordium and in conferring the determination of flower meristem. The gain-of-function of OsMADS1 transgenic lines presented the transformation of outer glumes to lemma-/palea-like organs and no changes in length of lemma and palea, but loss-of-function of OsMADS1 transgenic lines displayed the overdeveloped lemma and palea. Both findings revealed that OsMADS1 played a role in specifying lemma and palea and acted as a repressor of overdevelopment of lemma and palea. Moreover, it was indicated that OsMADS1 upregulated the transcript level of AP3 homologue OsMADS16, using real-time PCR analysis on gain- and loss-of-function of OsMADS1 transgenic lines.
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Abbreviations
- AG :
-
AGAMOUS
- AP :
-
APETALA
- DEF :
-
DEFICIENS
- GLO :
-
GLOBOSA
- nsr :
-
Naked seed rice
- PI :
-
PISTILLATA
- SEM:
-
Scanning electron microscopy
- SEP :
-
SEPALLATA
- SQUA :
-
SQUAMOSA
References
Angenent GC, Colombo L (1996) Molecular control of ovule development. Trends Plant Sci 1:228–232
Bustin SA (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169–193
Chuang CF, Meyerowitz EM (2000) Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proc Natl Acad Sci USA 97:4985–4990
Chung YY, Kim SR, Finkel D, Yanofsky MF, An G (1994) Early flowering and reduced apical dominance result from ectopic expression of a rice MADS box gene. Plant Mol Biol 26:657–665
Clark SE, Running MP, Meyerowitz EM (1993) CLAVATA1, a regulator of meristem and flower development in Arabidopsis. Development 119:397–418
Coen ES, Meyerowitz EM (1991) War of the whorls: genetic interactions controlling flower development. Nature 353:31–37
Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6:271–282
Honma T, Goto K (2001) Complexes of MADS-box proteins are sufficient to convert leaves into floral organs. Nature 409: 525–529
Jeon JS, Jang S, Lee S, Nam J, Kim C, Lee SH (2000) Leafy hull sterile 1 is a homeotic mutation in a rice MADS Box gene affecting rice flower development. Plant Cell 128:871–884
Kang HG, Jeon JS, Lee S, An G (1998) Identification of class B and class C floral organ identity genes from rice. Plant Mol Biol 38:1021–1029
Kempin SA, Savidge B, Yanofsky MF (1995) Molecular basis of the cauliflower phenotype in Arabidopsis. Science 267:522–525
Khush GS, Librojo AJ (1985) Naked seed rice (NSR) is allelic to op and lhs. Rice Genet News 2:71
Kinoshita T, Hidano Y, Takahashi M (1977) A mutant “long hull sterile” found in the rice variety, Sorachi. Memoirs of Faculty and Agriculture, Hokkaido University 10: 247–268
Kyozuka J, Kobayashi T, Morita M, Shimamoto K (2000) Spatially and temporally regulated expression of rice MADS box genes with similarity to Arabidopsis class A, B and C genes. Plant Cell Physiol 41:710–718
Laux T, Meyer KFX, Berger K, Jürgens G (1996) The WUSCHEL gene is required for shoot and floral meristem integrity in Arabidopsis. Development 122:87–96
Lee S, Jeon JS, An K, Moon YH, Lee S, Chung YY, An G (2003) Alteration of floral organ identity in rice through ectopic expression of OsMADS16. Planta 217:904–911
Lim J, Moon YH, An G, Jang SK (2000) Two rice MADS domain proteins interact with OsMADS1. Plant Mol Bio 44:513–527
Mandel MA, Gustafson BC, Savidge B, Yanofsky MF (1992) Molecular characterization of the Arabidopsis floral homeotic gene APETALA1. Nature 360:273–277
Moon YH, Jung JY, Kang HG, An G (1999) Identification of a rice APETALA3 homologue by yeast two-hybrid screening. Plant Mol Biol 40:167–177
Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, Nagato Y (2002) SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice. Development 130:705–718
Pelaz S, Gary SD, Baumann E, Wisman E, Yanofsky MF (2000) B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature 405:200–203
Pellegrini L, Tan S, Richmond TJ (1995) Structure of serum response factor core bound to DNA. Nature 376:490–498
Prasad K, Sriram P, Kumar CS, Kushalappa K, Vijayraghavan U (2001) Ectopic expression of rice OsMADS1 reveals a role in specifying the lemma and palea, grass floral organs analogous to sepals. Dev Genes Evol 211:281–290
Running MP, Meyerowitz EM (1996) Mutations in the PERIANTHIA gene of Arabidopsis specifically alter floral organ number and initiation pattern. Development 122:1261–1269
Running MP, Fletcher JC, Meyerowitz EM (1998) The WIGGUM gene is required for proper regulation of floral meristem size in Arabidopsis. Development 125:2545–2553
Schmidt R, Ambrose BA (1998) The blooming of grass flower development. Curr Opin Plant Biol 1:60–67
Suzaki T, Sato M, Ashikari M, Miyoshi M, Nagato Y, Hirano H (2004) The gene FLORAL ORGAN NUMBER1 regulates floral meristem size in rice and encodes a leucine-rich repeat receptor kinase orthologous to Arabidopsis CLAVATA1. Development 131:5649–5657
Tang XH, Zhu ZP, Huang QL (1981) Some aspects of morphological and physiological characters of ‘naked seed’ rice. Acta Genetica Sinica 8:350–355
Theissen G, Saedler H (2001) Floral quartets. Nature 409:469–471
Thomas J (2001) Relearning our ABCs: new twists on an old model. Trends Plant Sci 6:310–316
Weigel D, Meyerowitz EM (1994) The ABCs of floral homeotic genes. Cell 78: 203–209
Wu JG, Shi CH, Chen SY, Xiao JF (2004) The cytological mechanism of low fertility in the naked seed rice. Genetica 121:259–267
Xiao H, Wang Y, Liu D, Wang W, Li X, Zhao X, Xu J, Zhai W, Zhu L (2003) Functional analysis of the rice AP3 homologue OsMADS16 by RNA interference. Plant Mol Biol 52:957–966
Yamaguchi T, Nagasawa N, Kawasaki S, Matsuoka M, Nagato Y, Hirano HY (2004) The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa. Plant Cell 16:500–509
Yokoyama R, Nishitani K (2001) A comprehensive expression analysis of all the members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis. Plant Cell Physiol 42:1025–1033
Acknowledgements
This work was supported by National Natural Science Foundation of China (no. 30500319 and no. 30240030), the Science and Technology Office of Zhejiang Province (no. 011102471) and 151 Foundation for the Talents of Zhejiang Province.
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Chen, ZX., Wu, JG., Ding, WN. et al. Morphogenesis and molecular basis on naked seed rice, a novel homeotic mutation of OsMADS1 regulating transcript level of AP3 homologue in rice. Planta 223, 882–890 (2006). https://doi.org/10.1007/s00425-005-0141-8
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DOI: https://doi.org/10.1007/s00425-005-0141-8