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Agropyron elongatum chromatin localization on the wheat chromosomes in an introgression line

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Abstract

The introgressed small-chromosome segment of Agropyron elongatum (Host.) Neviski (Thinopyrum ponticum Podp.) in F5 line II-1-3 of somatic hybrid between common wheat (Triticum aestivum L.) and A. elongatum was localized by sequential fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and karyotype data. Karyotype analysis offered basic data of arm ratios and relative lengths of 21 pairs of chromosomes in parent wheat Jinan177 and hybrid II-1–3. Using special high repetitive sequences pSc119.2 and pAs1 for FISH, the entire B- and D-genome chromosomes were detected. The FISH pattern of hybrid II-1-3 was the same as that of parent wheat. GISH using whole genomic DNA from A. elongatum as probe determined the alien chromatin. Sequential GISH and FISH, in combination with some of the karyotype data, localized the small chromosome segments of A. elongatum on the specific sites of wheat chromosomes 2AL, 1BL, 5BS, 1DL, 2DL and 6DS. FISH with probe OPF-031296 from randomly amplified polymorphic DNA (RAPD) detected E-genome chromatin of A. elongatum, which existed in all of the small chromosome segments introgressed. Microsatellite primers characteristic for the chromosome arms above were used to check the localization and reveal the genetic identity. These methods are complementary and provide comprehensive information about the genomic constitution of the hybrid. The relationship between hybrid traits and alien chromatin was discussed.

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References

  • Bijoya B, Aniruddha PS, Hari SG (1999) Transfer of wild abortive cytoplasmic male sterility through protoplast fusion in rice. Mol Breed 5:319–327

    Google Scholar 

  • Blanco A, Cenci A, Simeone R, Gadaleta A, Pignone D, Galasso I (2002) The cytogenetics and molecular characteristics of a translocated chromosome 1AS1AL-1DL with a GLU-D1 locus in durum wheat. Cell Mol Biol Lett 7:559–567

    Google Scholar 

  • Chen SY, Xia GM, Quan T, Xiang F, Chen HM (2004) Studies on the salt-tolerance of F3–F6 hybrid Lines orginated from somatic hybridization between common wheat and Thinopyrum ponticum. Plant Science 167:773–779

    Google Scholar 

  • Doyle JJ, Doyle JI (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Forsberg J, Dixelius C, Lagercrantz U, Glimelius K (1998) UV dose-dependent DNA elimination in asymmetric somatic hybrids between Brassica napus and Arabidopsis thaliana. Plant Sci 131:65–76

    Google Scholar 

  • Forsström PO, Merker A, Schwarzacher T (2002) Characterization of mildew resistant wheat-rye substitution lines and identification of an inverted chromosome by fluorescent in situ hybridization. Heredity 88(5):349–355

    Google Scholar 

  • Gill BS (1987) Chromosome banding methods standard chromosome nomenclature and applications in cytogenetic analysis. In: Heyne EG (eds) Wheat and wheat improvement, 2nd edn. American Society of Agronomy, Madison, pp 243–254

    Google Scholar 

  • Hall RD, Rouwendal GJA, Krens FA (1992) Asymmetric somatic cell hybridization in plants II. Electrophoretic analysis of radiation-induced DNA damage and repair following exposure of sugarbeet (Beta vulgaris L.) protoplasts to UV and garmma rays. Mol Gen Genet 234:315–324

    Google Scholar 

  • Jiang J, Chen P, Fribe B (1993) Alloplasmic wheat-Elymus ciliaris chromosome addition lines. Genome 37:327–333

    Google Scholar 

  • Ko JM, Seo BB, Suh DY, Do GS, Park DS, Kwack YH (2002) Production of a new wheat line possessing the 1BL1RS wheat-rye translocation derived from Korean rye cultivar Paldanghomil. Theor Appl Genet 104:171–176

    Google Scholar 

  • Malysheva L, Sjakste T, Matzk F, Roder M, Ganal M (2003) Molecular cytogenetic analysis of wheat–barley hybrids using genomic in situ hybridization and barley microsatellite markers. Genome 46(2):314–322

    Google Scholar 

  • Mukai Y, Nakahara Y, Yamamoto M (1993) Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome 36:489–494

    Google Scholar 

  • Nagy ED, Molnár-Láng M, Linc G, Láng L (2002) Identification of wheat-barley translocations by sequential GISH and two-colour FISH in combination with the use of genetically mapped barley SSR markers. Genome 45:1238–1247

    Google Scholar 

  • Pedersen C, Langridge P (1997) Identification of the entire chromosome complement of bread wheat by two-colour FISH. Genome 40:589–593

    Google Scholar 

  • Ren ZL, Zhang HQ (1997) Induction of small-segment-transposition between wheat and rye chromosomes. Sci China Ser C 40(3):323–331

    Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Sear ER (1969) Wheat cytologenetics. Annu Rev Genet 3:451–468

    Google Scholar 

  • Waara S, Glimelius K (1995) The potential of somatic hybridization in crop breeding. Euphytica 85:217–223

    Google Scholar 

  • Wang YP, Sonntag K, Rudloff E (2003) Development of rapeseed with high erucic acid content by asymmetric somatic hybridization between Brassica napus and Crambe abyssinica. Theor Appl Genet 106:1147–1155

    CAS  PubMed  Google Scholar 

  • Wang J, Xiang FN, Xia GM (2004) Transfer of small chromosome fragments of Agropyron elongatum to wheat chromosome via asymmetric somatic hybridization. Sci China 47(4):1–8

    Google Scholar 

  • Wei YM, Zheng YL, Zhou RH (1999) Detection of the rye chromatin in multispikelet wheat germplasm 10-A background using fluorescence in situ hybridization FISH and RFLP markers. Acta Bot Sin 41(7):722–725

    Google Scholar 

  • Xia GM, Chen HM (1996) Plant regeneration from intergeneric somatic hybridization between Triticum aestivum L. and Leymus chinensis Trin. Tzvel. Plant Sci 120:197–203

    Article  CAS  Google Scholar 

  • Xia GM, Xiang FN, Zhou AF, Wang H, Chen HM (2003) Asymmetric somatic hybridization between Wheat (Triticum aestivum L) and Agropyron elongatum Host Nevishi. Theor Appl Genet 107:299–305

    Article  CAS  PubMed  Google Scholar 

  • Xiang FN, Xia GM, Chen HM (2003) Effect of UV dosage on somatic hybridization between common wheat (Triticum aestivum L.) and Avena sativa L. Plant Sci 164:697–707

    Article  CAS  Google Scholar 

  • Xiang FN, Xia GM, Zhi DY, Wang J, Nie H, Chen HM (2004) Hybrid plant regeneration in relation to the nuclear and cytoplasmic genomes of wheat and Setaria italica. Genome 47(4):680–688

    Google Scholar 

  • Xu J, Conner RL, Laroche A (1994) C-banding and florescence in situ hybridization studies of the wheat-alien hybrid Agrotana. Genome 37:477–481

    Google Scholar 

  • Xu CH, Xia GM, Zhi DY, Xiang FN, Chen HM (2003) Integration of maize nuclear and mitochondrial DNA into the wheat genome through somatic hybridization. Plant Sci 165:1001–1008

    Article  CAS  Google Scholar 

  • Ying J, Li B, Mu SM, Zhou HP, Liu JZ, Li ZS (2001) Identification of blue-grained wheat translocation lines using fluorescence in situ hybridization. Acta Bot Sin 43(2):164–168

    Google Scholar 

  • Yue W, Xia GM, Zhi D Y, Chen HM (2001) Transfer of salt tolerance from Aeleuropus littoralis Sinensis to wheat (Triticum aestivum L.) via asymmetric somatic hybridization. Plant Sci 161:259–266

    Article  CAS  PubMed  Google Scholar 

  • Zhang XY (1991) Production and utilization of alien translocation lines of common wheat. Heredita China 13(5):39–44

    Google Scholar 

  • Zhang XY, Dong YC (1999) Genome composition of Thinopyrum and rules of new species formation. J Yunnan Univ (Nat Sci) 21:66–67

    Google Scholar 

  • Zhang XY, Li DY (2000) Repetitive DNA sequences in wheat and its relatives. Sci Agric Sin 33 (5) :1–7

    Google Scholar 

  • Zhang XY, Dong YC, Li P, Richard R-C Wang (1998) Distribution of E- and St- specific RAPD fragments in few genomes of Triticeae. Acta Genet Sin 25(2):131–141

    Google Scholar 

  • Zhang ZY, Xin ZY, Larkin PJ (2001) Molecular characterization of a Thinopyrum intermedium Group 2 chromosome (2Ai-2) conferring resistance to barley yellow dwarf virus. Genome 44:1129–1135

    Google Scholar 

  • Zhao TJ, Quan TY, Xia GM, Chen HM (2003) Glutenin and SDS sedimentation analysis of the F5 somatic hybrids between Triticum aestivum and Agropyron elongatum. J Shandong Univ 38(3):112–116

    CAS  Google Scholar 

  • Zhou AF, Xia GM, Chen HM (2001) Comparative study of symmetric and asymmetric somatic hybridization between common wheat and Haynaldia villosa. Sci China Ser C 31(4):298–305

    Google Scholar 

  • Zhuang LF, Qi ZJ, Ying J, Chen PD, Liu DJ (2003) Development and identification of a set of Triticum aestivum–Thinopyrum bessarabicum disomic alien addition lines. Acta Genet Sin 30(10):919–925

    Google Scholar 

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Acknowledgements

J. Wang and F. Xiang contributed equally to this work. The National Natural Science Foundation of China, No.30370857, Major Project of Ministry of Education in China and National 863 High Technology Research and Development Project No. 2001AA241032 supported this study. We are grateful to Dr. Zhang Xueyong (Chinese Academy of Agriculture Sciences) for providing repeat sequences of B and D genome and control materials.

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  1. Jing Wang

    Present address: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

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  1. School of Life Sciences, Shandong University, Jinan, 250100, People’s Republic of China

    Jing Wang, Fengning Xiang & Guangmin Xia

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  1. Jing Wang
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  2. Fengning Xiang
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  3. Guangmin Xia
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Correspondence to Guangmin Xia.

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Wang, J., Xiang, F. & Xia, G. Agropyron elongatum chromatin localization on the wheat chromosomes in an introgression line. Planta 221, 277–286 (2005). https://doi.org/10.1007/s00425-004-1443-y

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