Abstract
By immunoblotting with anti-human integrin β polyclonal antibodies (β1, β3 or β5), a single distinct band of about 60 kDa was detected in total protein extracts from mature leaves of the seagrass Zostera marina L., but no band was detected in total protein extracts from immature seagrass leaves, freshwater grass leaves or Arabidopsis thaliana (L.) Heynh. leaves. This integrin-like protein was detected by indirect immunofluorescence microscopy on the surface of non-spherical protoplasts of epidermal cells isolated from mature seagrass leaves using an anti-integrin β3 polyclonal antibody. Electron-microscopic analyses with the same antibody indicated that this integrin-like protein was localized specifically in the invaginated plasma membrane of epidermal cells in mature seagrass leaves. Therefore, this integrin-like protein of about 60 kDa may be involved in the developmentally regulated invagination of the plasma membrane in epidermal cells of the seagrass Z. marina.
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Abbreviations
- CM :
-
Crude microsomal
- ECM :
-
Extracellular matrix
- FITC :
-
Fluorescein isothiocyanate
- PM :
-
Plasma membrane
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Acknowledgements
We thank Dr. S. Takagi of Osaka University for his useful suggestions and valuable advice. This research was supported in part by grants from the Salt Science Research Foundation (No. 9822) and the Tokyu Foundation (No. 207) to T.F., and by a Sasakawa Scientific Research Grant (No. 14-372MK) to Y.M. from the Japan Science Society.
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Sakurai, M., Pak, JY., Muramatsu, Y. et al. Integrin-like protein at the invaginated plasma membrane of epidermal cells in mature leaves of the marine angiosperm Zostera marina L.. Planta 220, 271–277 (2004). https://doi.org/10.1007/s00425-004-1348-9
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DOI: https://doi.org/10.1007/s00425-004-1348-9