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The unusual Arabidopsis extensin gene atExt1 is expressed throughout plant development and is induced by a variety of biotic and abiotic stresses

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Abstract

We detail the expression of the Arabidopsis thaliana (L.) Heynh. atExt1 extensin gene. atExt1 is normally expressed in roots and inflorescences, and is induced by wounding, exogenously supplied salicylic acid, methyl jasmonate, auxins and brassinosteroids. Northern assays and histochemical analysis of transgenics expressing an atExt1::gus fusion show that this gene is also induced by the brassica pathogen Xanthomonas campestris pv. campestris and that this induction is restricted to tissues close to the site of infection. Expression at regions of abscission and senescence also implicates atExt1 in these important developmental processes.

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Fig. 1.
Fig. 2.
Fig. 3A, B.
Fig. 4A–O.
Fig. 5A–C.
Fig. 6A–L.
Fig. 7.
Fig. 8.

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Abbreviations

GUS:

β-glucuronidase gene, uidA

HR:

hypersensitive response

MeJA:

methyl jasmonate

SA:

salicylic acid

SAR:

systemic acquired resistance

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Acknowledgements

G.M. was supported by the State Scholarship Foundation of Greece. We thank Daniel Price currently at the School of Biological Sciences, University of Durham, Durham UK for the data presented in Fig. 8. We also thank Prof. Mike Daniels (The Sainsbury Laboratory, John Innes Centre, Norwich, UK) for the gift of Xanthomonas campestris pv. campestris

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  1. Georgios Merkouropoulos

    Present address: Sainsbury Laboratory, Colney, Norwich, NR4 7UH, UK

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  1. School of Biological Sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, UK

    Georgios Merkouropoulos & Anil H. Shirsat

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  1. Georgios Merkouropoulos
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Correspondence to Anil H. Shirsat.

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Merkouropoulos, G., Shirsat, A.H. The unusual Arabidopsis extensin gene atExt1 is expressed throughout plant development and is induced by a variety of biotic and abiotic stresses. Planta 217, 356–366 (2003). https://doi.org/10.1007/s00425-003-1002-y

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