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Basolateral localization of flounder Na+-dicarboxylate cotransporter (fNaDC-3) in the kidney of Pleuronectes americanus

  • Ion Channels, Transporters
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Abstract

The purpose of this study was to provide functional and immunocytochemical evidence for the location of the winter flounder (Pleuronectes americanus) sodium-dicarboxylate cotransporter-3 (fNaDC-3) in the basolateral membrane of proximal tubule cells. fNaDC-3 was expressed in Xenopus laevis oocytes. Lowering the external pH from 7.5 to 6.5 or 5.5 modestly decreased the uptake of [14C]succinate into fNaDC-3 expressing oocytes, but markedly increased the uptake of [14C]citrate. As measured by the two-electrode voltage-clamp technique, the citrate concentration eliciting half-maximal current, K 0.5, decreased from 490 µM at pH 7.5 to 32 µM at pH 6.0. The maximal inwards current, ΔI max, increased from −27 to −72 nA, when bath pH was changed from 7.5 to 6.0. These data suggest that fNaDC-3 translocates preferably divalent citrate. cis-Aconitate, a tricarboxylate that interacts exclusively with basolateral sodium-dicarboxylate cotransport in the rat kidney, was translocated by fNaDC-3 with a K 0.5 of 300 µM. Antibodies raised against an NaDC-3-specific peptide reacted with the basal cell side of flounder renal proximal tubule segment II (PII). No other structures were stained, indicating that fNaDC-3 is located exclusively in the basolateral membrane of PII cells. We assume that fNaDC-3 provides PII cells with Krebs cycle intermediates as fuels and with α-ketoglutarate to drive organic anion secretion.

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Acknowledgements

This research was supported by DFG Bu 998/2-1 to B.C.B. and by DFG Ste 435/2-4 to J.S.

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Authors and Affiliations

  1. Zentrum Physiologie und Pathophysiologie, Abteilung Vegetative Physiologie und Pathophysiologie, Georg-August-Universität Göttingen, Humboldtallee 23, 37073, Göttingen, Germany

    Birgitta C. Burckhardt*, Lars Kühne, Gerhard Burckhardt & Jürgen Steffgen

  2. Max-Planck Institut für molekulare Physiologie, Otto-Hahn-Strasse 11, 44227, Dortmund, Germany

    Hartmut Hentschel* & Beate Schölermann

  3. Mount Desert Island Biological Laboratory, Salesbury Cove, ME, USA

    Hartmut Hentschel* & Beate Schölermann

Authors
  1. Hartmut Hentschel*
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  2. Birgitta C. Burckhardt*
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  3. Beate Schölermann
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  4. Lars Kühne
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  5. Gerhard Burckhardt
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  6. Jürgen Steffgen
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Correspondence to Jürgen Steffgen.

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*H. Hentschel and B.C. Burckhardt contributed equally to this work

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Hentschel*, H., Burckhardt*, B.C., Schölermann, B. et al. Basolateral localization of flounder Na+-dicarboxylate cotransporter (fNaDC-3) in the kidney of Pleuronectes americanus . Pflugers Arch - Eur J Physiol 446, 578–584 (2003). https://doi.org/10.1007/s00424-003-1081-4

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  • DOI: https://doi.org/10.1007/s00424-003-1081-4

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