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Implantation of alloplastic material increases survival of mice subsequently exposed to polymicrobial sepsis

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Abstract

Purpose

Major surgery can modulate the immune system and by this the clinical course of following complications. Effects of minor surgical treatments on the immune system and septic complications are poorly understood.

Materials and methods

We investigated the effect of a minor surgical procedure—the implantation of an osmotic pump—on the outcome of experimental polymicrobial sepsis (colon ascendens stent-induced peritonitis, CASP) in mice.

Results

Animals with pumps implanted 3 days prior to CASP showed an attenuated clinical course of sepsis and increased survival. While measured serum cytokine levels were not affected by the minor surgical stress of pump implantation, splenocyte secretion of IFN-gamma in response to lipopolysaccharide was increased.

Conclusion

The early implantation of alloplastic material modulates the immune system and leads to an increased survival of a polymicrobial sepsis. Identifying the molecular nature of this effect might point the way to a new therapeutic approach to reduce sepsis mortality.

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Acknowledgments

The expert technical assistance of Kathrin Mülling and Antje Müller is gratefully acknowledged. This work was supported by DFG-GK 840 “Host pathogen interactions” and by the Bundesministerium für Forschung und Technologie (BMBF-NBL3, FKZ 01ZZ0403, Modul E5).

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Authors and Affiliations

  1. Klinik für Allgemeine Chirurgie, Visceral-, Thorax- und Gefäßchirurgie der Ernst-Moritz-Arndt-Universität Greifswald, Friedrich-Loeffler-Str. 23b, 17457, Greifswald, Germany

    Wolfram Kessler, Tobias Ebker, Pia Koerner, Katharina Cziupka, Tobias Traeger, Alexandra Westerholt, Hendrik Mehmcke, Friederike Neher, Claus-Dieter Heidecke & Stefan Maier

  2. Department of Immunology, Ernst-Moritz-Arndt-Universität Greifswald, Ferdinand-Sauerbruch-Straße, 17475, Greifswald, Germany

    Christian Poetschke & Robert S. Jack

Authors
  1. Wolfram Kessler
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  2. Tobias Ebker
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  3. Pia Koerner
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  4. Christian Poetschke
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  5. Katharina Cziupka
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  6. Tobias Traeger
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  7. Alexandra Westerholt
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  8. Hendrik Mehmcke
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  9. Friederike Neher
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  10. Robert S. Jack
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  11. Claus-Dieter Heidecke
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  12. Stefan Maier
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Corresponding author

Correspondence to Wolfram Kessler.

Additional information

Kessler and Ebker contributed equally to this work.

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Kessler, W., Ebker, T., Koerner, P. et al. Implantation of alloplastic material increases survival of mice subsequently exposed to polymicrobial sepsis. Langenbecks Arch Surg 395, 157–162 (2010). https://doi.org/10.1007/s00423-009-0463-2

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  • DOI: https://doi.org/10.1007/s00423-009-0463-2

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