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Negative association between serum dioxin level and oxidative DNA damage markers in municipal waste incinerator workers

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Abstract

Objectives: To investigate the effect of dioxin on the formation of oxidative DNA damage and urinary mutagenicity, we measured the concentrations of serum dioxins and lymphocytic 8-hydroxydeoxyguanosine (8-OH-dG) in 57 male waste incinerator workers, urinary 8-OH-dG and urinary mutagenicity in 29 male waste incinerator workers. Methods: Information about the subjects was obtained from a questionnaire. Concentrations of polychlorinated dibenzo-p-dioxin (PCDD), polychlorinated dibenzofuran (PCDF), and coplanar-polychlorinated-biphenyl (Co-PCB) in serum samples from the workers were measured with a high-resolution gas chromatograph /high-resolution mass spectrometer. Lymphocytic and urinary 8-OH-dG levels were measured with a high-performance liquid chromatography-electrochemical detector system. The urinary mutagenicity was measured with umu assay. Results: The lymphocytic 8-OH-dG level showed a negative association with the serum dioxin level (total value of TEQ-PCDD, PCDF, and Co-PCB). Urinary 8-OH-dG did not show correlation with serum dioxin level, but showed positive correlation with the smoking index. Conclusions: With respect to the subjects‘ serum dioxin level, dioxin did not increase the urinary 8-OH-dG level by oxidative DNA damage, but upregulation of the primary defenses with oxidative damage and/or DNA repair system activity might have occurred.

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Abbreviations

8-OH-dG:

8-Hydroxydeoxyguanosine

PCDD:

Polychlorinated dibenzo-p-dioxin

TCDD:

2,3,7,8- Tetrachlorodibenzo-p-dioxin

PCDF:

Polychlorinated dibenzofuran

1,2,3,4,6,7,8-HpCDF:

1,2,3,4,6,7,8-Heptachlorodibenzofuran

Co-PCB:

Coplanar-polychlorinated-biphenyl

BMI:

Body mass index

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Authors and Affiliations

  1. Department of Environmental Health, Osaka Prefectural Institute of Public Health, Osaka, 1-3-69, Nakamichi, Higashinari-ku, 537-0025, Japan

    Jin Yoshida, Shinji Kumagai, Takeo Tabuchi, Hiroshi Kosaka, Susumu Akasaka & Hajime Oda

  2. Department of Environmental Oncology, University of Occupational and Environmental Health, Kitakyushu, Japan

    Hiroshi Kasai

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  1. Jin Yoshida
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  2. Shinji Kumagai
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  3. Takeo Tabuchi
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  4. Hiroshi Kosaka
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  5. Susumu Akasaka
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  6. Hiroshi Kasai
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  7. Hajime Oda
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Correspondence to Jin Yoshida.

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Yoshida, J., Kumagai, S., Tabuchi, T. et al. Negative association between serum dioxin level and oxidative DNA damage markers in municipal waste incinerator workers. Int Arch Occup Environ Health 79, 115–122 (2006). https://doi.org/10.1007/s00420-005-0035-x

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  • DOI: https://doi.org/10.1007/s00420-005-0035-x

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