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Developmental function of Piezo1 in mouse submandibular gland morphogenesis

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Abstract

Mechanically activated factors are important in organogenesis, especially in the formation of secretory organs, such as salivary glands. Piezo-type mechanosensitive ion channel component 1 (Piezo1), although previously studied as a physical modulator of the mechanotransduction, was firstly evaluated on its developmental function in this study. The detailed localization and expression pattern of Piezo1 during mouse submandibular gland (SMG) development were analyzed using immunohistochemistry and RT-qPCR, respectively. The specific expression pattern of Piezo1 was examined in acinar-forming epithelial cells at embryonic day 14 (E14) and E16, which are important developmental stages for acinar cell differentiation. To understand the precise function of Piezo1 in SMG development, siRNA against Piezo1 (siPiezo1) was employed as a loss-of-function approach, during in vitro organ cultivation of SMG at E14 for the designated period. Alterations in the histomorphology and expression patterns of related signaling molecules, including Bmp2, Fgf4, Fgf10, Gli1, Gli3, Ptch1, Shh, and Tgfβ-3, were examined in acinar-forming cells after 1 and 2 days of cultivation. Particularly, altered localization patterns of differentiation-related signaling molecules including Aquaporin5, E-cadherin, Vimentin, and cytokeratins would suggest that Piezo1 modulates the early differentiation of acinar cells in SMGs by modulating the Shh signaling pathway.

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Funding

This study was supported by the National Research Foundation of Korea (grant nos. NRF-2019R1I1A3A01062091 and NRF-2022R1I1A2063745) and is funded by the Ministry of Education, Science, and Technology, South Korea.

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Authors and Affiliations

  1. Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea

    Elina Pokharel, Yam Prasad Aryal, Tae-Young Kim, Anna Kim & Jae-Young Kim

  2. Department of Dental Hygiene, College of Health Science, Gachon University, Incheon, 21936, South Korea

    Ji-Youn Kim

  3. Department of Histology and Developmental Biology, Tokyo Dental College, Tokyo, 101-0061, Japan

    Hitoshi Yamamoto

  4. Division of Anatomy and Developmental Biology, Department of Oral Biology, Yonsei University College of Dentistry, Seoul, 03722, South Korea

    Sung-Won Cho

  5. Department of K-Beauty Business, College of Cosmetics and Pharmaceuticals, Daegu Hanny University, Daegu, 38610, South Korea

    Wern-Joo Sohn

  6. Department of Oral Medicine, School of Dentistry, IHBR, Kyungpook National University, Daegu, 41940, South Korea

    Jae-Kwang Jung

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  1. Elina Pokharel
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  2. Yam Prasad Aryal
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  4. Anna Kim
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Contributions

P.E., K.J.Y., and J.J.K. contributed to conception, design, data acquisition, analysis, and interpretation. The authors also drafted and critically revised the manuscript. A.Y.P., K.T.Y., K.A., K.J.Y., Y.H., C.S.W., and S.W.J. contributed to the conception, analysis, and critical review of the manuscript. All authors gave their final approval and agreed to be accountable for all aspects of this work.

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Correspondence to Jae-Young Kim or Jae-Kwang Jung.

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Pokharel, E., Aryal, Y.P., Kim, TY. et al. Developmental function of Piezo1 in mouse submandibular gland morphogenesis. Histochem Cell Biol 159, 477–487 (2023). https://doi.org/10.1007/s00418-023-02181-w

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