Abstract
The apparent connection of galectin-3 to chondrocyte survival and osteoarthritis-like cartilage modifications in animal models provided incentive for the mapping of seven members of this family of adhesion/growth-regulatory proteins in human cartilage specimens. Starting with work in vitro, RT-qPCR analyses and immunocytochemistry revealed gene transcription and protein presence in cultured OA chondrocytes, especially for galectin-1, galectin-3 and galectin-8. Immunohistochemistry in clinical specimens with mild and severe cartilage degeneration detected galectins in chondrocytes—with upregulation, especially of galectin-1 in areas of severe degeneration—accompanied by α2,6-sialylation in the pericellular matrix. Given the possibility for additive/antagonistic activities between galectins, these results direct further research toward examining cellular effects of (1) these proteins (alone or in combination) on chondrocytes and (2) remodeling of the chondrocyte glycophenotype.
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Acknowledgments
This work was generously supported by EC funding (Grant agreements 260600 (GlycoHIT) and 317297 (GLYCOPHARM)) and the Verein zur Förderung des biologisch-technologischen Fortschritts in der Medizin e. V. (Heidelberg, Germany). Insightful discussions with Dr. J. Domingo-Ekark and Dr. B. Friday are gratefully acknowledged, as is the valuable input of the reviewers. Ruth Grübl-Barabas, Bettina Rodriguez-Molina and Melanie Cezanne are acknowledged for excellent technical assistance.
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Toegel, S., Bieder, D., André, S. et al. Human osteoarthritic knee cartilage: fingerprinting of adhesion/growth-regulatory galectins in vitro and in situ indicates differential upregulation in severe degeneration. Histochem Cell Biol 142, 373–388 (2014). https://doi.org/10.1007/s00418-014-1234-x
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DOI: https://doi.org/10.1007/s00418-014-1234-x