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Peroxisomes in dental tissues of the mouse

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Abstract

Patients with mild forms of peroxisomal biogenesis disorders show facial dysmorphism and exhibit dentition problems accompanied by enamel hypoplasia. However, no information is available on the role of peroxisomes in dental and paradontal tissues. Therefore, we studied the distribution of these organelles, their protein composition and the expression of corresponding genes during dental development and in mature decalcified teeth in mice. Perfusion-fixed heads of mice of different developmental stages (E13.5 to adult) were cut in sagittal direction into two halves and embedded in paraffin for serial sectioning and subsequent peroxidase-based immunohistochemistry or double-immunofluorescence preparations. Frozen, unfixed heads of newborn mice were used for cryosectioning and subsequent laser-assisted microdissection of ameloblasts and odontoblasts, RNA isolation and RT-PCR analysis. Our results revealed the presence of peroxisomes already in the bud stage of dental development. An increase in peroxisome abundance was noted during differentiation of ameloblasts and odontoblasts with the highest number of organelles in Tomes’ processes of mature ameloblasts. A strong heterogeneity of peroxisomal enzyme content developed within differentiated dental cell types. A drastic down-regulation of catalase in maturing ameloblasts was noted in contrast to high levels of lipid metabolizing enzymes in peroxisomes of these cells. As known from the literature, differentiated ameloblasts are more prone to oxidative damage which could be explained by the low catalase levels inside of this cell type.

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Abbreviations

PFA:

Paraformaldehyde

PBS:

Phosphate-buffered saline

CAT:

Catalase

ROS:

Reactive oxygen species

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Acknowledgments

The excellent technical assistance of Magdalena Gottwald is gratefully acknowledged. Further, we would like to thank Profs. Denis I.Crane, Steve Gould, Paul P. Van Veldhoven for providing us with some antibodies (see Table 1). Furthermore, we thank Dr. Martin Obert, Department of Neuroradiology of the University Hospital Marburg-Giessen for checking the decalcification of dental tissue by volumetric computer tomography (VCT). Finally, we are indebted to Michelle Woods for carefully reading and correcting the manuscript.

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  1. Division of Medical Cell Biology, Institute for Anatomy and Cell Biology II, Justus Liebig University, Aulweg 123, 35385, Giessen, Germany

    Ingra Stelzig, Srikanth Karnati, Klaus Peter Valerius & Eveline Baumgart-Vogt

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  1. Ingra Stelzig
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  2. Srikanth Karnati
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Correspondence to Eveline Baumgart-Vogt.

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Stelzig, I., Karnati, S., Valerius, K.P. et al. Peroxisomes in dental tissues of the mouse. Histochem Cell Biol 140, 443–462 (2013). https://doi.org/10.1007/s00418-013-1131-8

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