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Human periodontal ligament fibroblasts are the optimal cell source for induced pluripotent stem cells

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Abstract

Among the various kinds of fibroblasts existing in the human body, the periodontal ligament (PDL) fibroblasts have been suggested as multipotent cells. Periodontal ligament fibroblasts are characterized by rapid turnover, a high remodeling capacity and remarkable capacity for renewal and repair. They also differentiate into osteoblasts and cementoblasts. We established iPS cells from human PDL fibroblasts by introducing the ES cell markers Oct3/4, Sox2, Nanog, Klf4 and Lin28 by retrovirus transduction, even without the oncogene c-Myc. The iPS cells established in this study expressed the ES cell markers and formed teratomas in SCID mice. The c-Myc expression level in the PDL fibroblasts was higher than that in the iPS cells by quantitative RT-PCR. Therefore, we have concluded that PDL fibroblasts could be an optimal cell source for iPS cells.

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Abbreviations

PDL:

Periodontal ligament

HPDLF:

Human periodontal ligament fibroblasts

iPS cell:

Induced pluripotent stem cell

ES cell:

Embryonic stem cell

ALP:

Alkaline phosphatase

HPDL-iPS:

iPS cell derived from a HPDLF

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Authors and Affiliations

  1. Department of Translational Research, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama, Kanagawa, 230-8501, Japan

    Yoshiaki Nomura, Seetala Sanggarnjanavanich & Nobuhiro Hanada

  2. Department of Orthodontics, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama, Kanagawa, 230-8501, Japan

    Misao Ishikawa, Yuichi Yashiro, Seetala Sanggarnjanavanich, Chihiro Arai, Koji Noda & Yoshiki Nakamura

  3. Department of Operative Dentistry, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Kanagawa, 230-8501, Japan

    Takao Yamaguchi

  4. Section of Biostructural Science, Graduate School of Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan

    Yoshiro Takano

Authors
  1. Yoshiaki Nomura
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  2. Misao Ishikawa
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  3. Yuichi Yashiro
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  4. Seetala Sanggarnjanavanich
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  5. Takao Yamaguchi
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  6. Chihiro Arai
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  7. Koji Noda
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  8. Yoshiro Takano
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  9. Yoshiki Nakamura
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  10. Nobuhiro Hanada
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Correspondence to Yoshiaki Nomura.

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Nomura, Y., Ishikawa, M., Yashiro, Y. et al. Human periodontal ligament fibroblasts are the optimal cell source for induced pluripotent stem cells. Histochem Cell Biol 137, 719–732 (2012). https://doi.org/10.1007/s00418-012-0923-6

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