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MALDI imaging mass spectrometry for direct tissue analysis: technological advancements and recent applications

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Abstract

Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a method that allows the investigation of the molecular content of tissues within its morphological context. Since it is able to measure the distribution of hundreds of analytes at once, while being label free, this method has great potential which has been increasingly recognized in the field of tissue-based research. In the last few years, MALDI-IMS has been successfully used for the molecular assessment of tissue samples mainly in biomedical research and also in other scientific fields. The present article will give an update on the application of MALDI-IMS in clinical and preclinical research. It will also give an overview of the multitude of technical advancements of this method in recent years. This includes developments in instrumentation, sample preparation, computational data analysis and protein identification. It will also highlight a number of emerging fields for application of MALDI-IMS like drug imaging where MALDI-IMS is used for studying the spatial distribution of drugs in tissues.

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Acknowledgments

The authors thank Stephan Meding and Mareike Elsner for helping in editing this report. This work was supported by grants from the BMBF (No. 01EZ0803, No. 0315508A, and No. 01IB10004E) and the Deutsche Forschungsgemeinschaft (SFB 824 TP B1, SFB 824 TP Z2, and WA 1656/3-1).

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Authors and Affiliations

  1. Institute of Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstaedter Landstraße 1, 85764, Neuherberg, Germany

    Benjamin Balluff, Cedrik Schöne, Heinz Höfler & Axel Walch

  2. Department of Medicine II, Klinikum rechts der Isar, Technische Universität München, Munich, Germany

    Benjamin Balluff

  3. Institute of Pathology, Technische Universität München, Munich, Germany

    Heinz Höfler

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  1. Benjamin Balluff
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  2. Cedrik Schöne
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  3. Heinz Höfler
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  4. Axel Walch
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Correspondence to Axel Walch.

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B. Balluff and C. Schöne contributed equally to this work.

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Balluff, B., Schöne, C., Höfler, H. et al. MALDI imaging mass spectrometry for direct tissue analysis: technological advancements and recent applications. Histochem Cell Biol 136, 227–244 (2011). https://doi.org/10.1007/s00418-011-0843-x

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