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Mapping of CIP/KIP inhibitors, G1 cyclins D1, D3, E and p53 proteins in the rat term placenta

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Abstract

As cell cycle regulation is fundamental to the normal growth and development of the placenta, the aim of the present study was to determine the immunolocalizations of cell cycle related proteins, which have key roles in proliferation, differentiation and apoptosis during the development of the rat placenta. Here immunohistochemistry has been used to localize G1 cyclins (D1, D3, E), which are major determinants of proliferation, CIP/KIP inhibitors (p21, p27, p57), p53 as a master regulator and proliferating cell nuclear antigen in all cell types of the rat term placenta. The proportion of each cell type immunolabeled was counted. Cyclin D1 and cyclin D3 were present mostly in cells of the fetal aspect of the placenta, whereas the G1/S cyclin E was present only in the spongio- and labyrinthine trophoblast populations. Among the CIP/KIP inhibitors, p21 was present only in cells of the fetal aspect whereas p27 and p57 were found in all cell types studied. p53 was only found in a small proportion of cells with no co-localization of p53 and p21. The data suggest that the cells of the fetal side of the rat placenta still have some proliferation potential which is kept in check by expression of the CIP/KIP cell cycle inhibitors, whereas cells of the maternal aspect have lost this potential. Apoptosis is only marginal in the term rat placenta. In conclusion, proliferation and apoptosis in rat placental cells appears controlled mostly by the CIP/KIP inhibitors in late pregnancy.

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Acknowledgments

The project was funded by the Commission of the European Communities (QLK1-2001-00138 PERILIP), but does not necessarily reflect its views or anticipate its further policy. Emin Turkay Korgun was partly supported by Research Found of Akdeniz University, Antalya, Turkey (project number: 2008.02.0122.008). The authors thank Milagros Morante for her excellent technical assistance.

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Authors and Affiliations

  1. Department of Histology and Embryology, Medical Faculty, Akdeniz University, 07070, Antalya, Turkey

    Emin Turkay Korgun & Gozde Unek

  2. Department of Biochemistry, Medical Faculty, Akdeniz University, 07070, Antalya, Turkey

    Dijle Kipmen-Korgun

  3. Department of Biochemistry and Molecular Biology, University of San Pablo-CEU, Madrid, Spain

    Emilio Herrera

  4. Department of Obstetrics and Gynecology, Medical University of Graz, Graz, Austria

    Christian Wadsack & Gernot Desoye

  5. Maternal and Fetal Health Research Centre, MAHSC, University of Manchester, St. Mary’s Hospital, Manchester, UK

    Carolyn J. Jones

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  1. Emin Turkay Korgun
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Correspondence to Emin Turkay Korgun.

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Korgun, E.T., Unek, G., Herrera, E. et al. Mapping of CIP/KIP inhibitors, G1 cyclins D1, D3, E and p53 proteins in the rat term placenta. Histochem Cell Biol 136, 267–278 (2011). https://doi.org/10.1007/s00418-011-0841-z

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