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Molecular composition of tight and adherens junctions in the rat olfactory epithelium and fila

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Abstract

Tight and adherens junctions (TJs, AJs) between neurons, epithelial and glial cells provide barrier and adhesion properties in the olfactory epithelium (OE), and subserve functions such as compartmentalization and axon growth in the fila olfactoria (FO). Immunofluorescence and immunoelectronmicroscopy were combined in sections of rat OE and FO to document the cellular and subcellular localization of TJ proteins occludin(Occl), claudins(Cl) 1–5 and zonula occludens(ZO) proteins 1–3, and of AJ proteins N-cadherin(cad), E-cad, and alpha-, beta- and p120-catenin(cat). With the exception of Cl2, all TJ proteins were colocalized in OE junctions. Differences in relative immunolabeling intensities were noted between neuronal and epithelial TJs. In the FO, Cl5-reactivity was localized in olfactory ensheathing cell (OEC) junctions, Cl1-reactivity in the FO periphery, with differential colocalization with ZOs. Supporting cells formed N-cad-immunoreactive (ir) AJs with olfactory sensory neurons, E-cad-ir junctions with microvillar and gland duct cells, and both N-cad and E-cad-ir junctions in homotypic contacts. Alpha, β- and p120-cat were localized in all AJs of the OE. AJs were scarce in the globose basal cell layer. Immature and mature neurons formed numerous contacts. In the FO, AJs were documented between OECs, between OECs and axons, and between axons. Most AJs colocalized N-cad with catenins, occasionally E-cad-ir AJs were found in the FO periphery. Characteristics of molecular composition suggest differential properties of TJs formed by neuronal, epithelial and glial cells in the OE and FO. The presence and molecular composition of AJs are consistent with a role of AJ proteins in neuroplastic processes in the peripheral olfactory pathway.

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Abbreviations

AJ:

Adherens junction

AMCA:

7-Amino-4-methylcoumarin-3-acetic acid

BC:

Basal cell

BCL:

Basal cell layer

BSA:

Bovine serum albumin

β-TUB:

Beta-tubulin

βIII-TUB:

Neuron specific β-tubulin, βIII isotype

Cad:

Cadherin

Cat:

Catenin

Cl:

Claudin

CK:

Cytokeratin

EDC:

Excretory duct cell

FO:

Fila olfactoria

FI:

Fluorescence immunohistochemistry

g:

Green fluorescent

GA:

Glutaraldehyde

GAP43:

Growth associated protein 43

GBC:

Globose basal cell

GFAP:

Glial fibrillary acidic protein

HBC:

Horizontal basal cell

-ir:

-immunoreactive

LP:

Lamina propria

m:

Monoclonal

MC:

Microvillar cell

NDS:

Normal donkey serum

NGS:

Normal goat serum

OB:

Olfactory bulb

Occl:

Occludin

OE:

Olfactory epithelium

OEC:

Olfactory ensheathing cell

OG:

Olfactory gland

ONF:

Olfactory nerve fibroblast

ONL:

Olfactory nerve layer

OMP:

Olfactory marker protein

OSN:

Olfactory sensory neuron

OSNL:

Olfactory sensory neuron layer

p:

Polyclonal

p120:

p120-catenin

PE:

Postembedding immunogold labeling

PFA:

Paraformaldehyde

PBS:

Phosphate buffered saline

r:

Red fluorescent

RT:

Room temperature

SC:

Supporting cell

SCL:

Supporting cell layer

TJ:

Tight junction

V:

Vessel

ZO:

Zonula occludens protein

ZO1m:

Rat monoclonal anti-ZO1

ZO1rp:

Rabbit polyclonal anti-ZO1

ZO1gp:

Goat polyclonal anti-ZO1

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Acknowledgments

The authors are deeply indebted to Rita Hermann, Sieglinde Schenk and Karin Reinfurt for technical support, and to Prof. Frank Margolis, Baltimore, and Prof. James Schwob, Boston, for their generous gift of antibodies. The studies were supported by the Deutsche Forschungsgemeinschaft, SFB 581, TPZ3.

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  1. Institute of Anatomy and Cell Biology, University of Wuerzburg, Koellikerstr. 6, 97070, Wuerzburg, Germany

    Axel Steinke, Sofia Meier-Stiegen, Detlev Drenckhahn & Esther Asan

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  1. Axel Steinke
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Correspondence to Esther Asan.

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Steinke, A., Meier-Stiegen, S., Drenckhahn, D. et al. Molecular composition of tight and adherens junctions in the rat olfactory epithelium and fila. Histochem Cell Biol 130, 339–361 (2008). https://doi.org/10.1007/s00418-008-0441-8

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