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Localization of vascular endothelial growth factor in the zona pellucida of developing ovarian follicles in the rat: a possible role in destiny of follicles

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Abstract

There is increasing evidence that in many species angiogenic factors, such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), may have important roles in folliculogenesis. The aim of this study is to determine the localization of VEGF and its receptors, Flt-1 and KDR, and bFGF expression in the rat ovary and to evaluate their distributions throughout the different follicular stages. Out of 20 virginal female rats, 10 were studied during the natural ovarian cycle without any ovulation induction. The other 10 were superovulated and their ovaries were studied by western analysis and immunohistochemistry. Granulosa cells (GC) and oocytes of primordial follicles were negative for VEGF. In early primary follicles, VEGF was present in the oocyte but its immunoreactivity was weak, while newly developing zona pellucida (ZP) of primary follicles was negative for VEGF. Subsequently, with the commencement of antral spaces between GC of the secondary follicle, ZP of some secondary follicles became strongly positive for VEGF, forming a continuous ring around the oocyte. In preovulatory mature follicles granulosa and theca interna (TI) cells showed a weak immunoreactivity for VEGF. Western blot analyses have also demonstrated that VEGF, a 26-kDa protein, was present in follicles. Moreover, in ovulated cumulus–oocyte complex we observed a halo-like immunoreactivity of VEGF around the fully mature oocyte. The immunoreactivity for Flt-1 and KDR receptors in growing follicles was mostly limited to GC and TI cells. Anti-bFGF did not exhibit any immunoreactivity in ZP of follicles at any stage. Its expression was weak in GC of the follicles at different stages, whereas, it could be localized to some extent in the blood capillaries of TI of antral follicles and in blood vessels localized in the stroma. Interestingly, VEGF immunoreactivity in the ZP of some secondary follicles is very striking. Accordingly, the possibility that VEGF may be an important regulatory molecule for the dominant follicle selection or atresia should be considered.

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Fig. 1 Fig. 2a–c Fig. 3 Fig. 4a, b Fig. 5
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Acknowledgements

We wish to thank to Sibel OZER for her excellent technical assistance. This work was partly supported by Akdeniz University Research Fund, Antalya, Turkey, grant number AKD−20.01.0103.

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Authors and Affiliations

  1. Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, 07070, Antalya, Turkey

    Ciler Celik-Ozenci, Gokhan Akkoyunlu, Umit Ali Kayisli & Ramazan Demir

  2. Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06520-8063, USA

    Ciler Celik-Ozenci, Umit Ali Kayisli & Aydin Arici

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  1. Ciler Celik-Ozenci
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  2. Gokhan Akkoyunlu
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Correspondence to Ciler Celik-Ozenci.

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Celik-Ozenci, C., Akkoyunlu, G., Kayisli, U.A. et al. Localization of vascular endothelial growth factor in the zona pellucida of developing ovarian follicles in the rat: a possible role in destiny of follicles. Histochem Cell Biol 120, 383–390 (2003). https://doi.org/10.1007/s00418-003-0586-4

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