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Role of interleukin-1β in hypoxia-induced depression of glutamate uptake in retinal Müller cells

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Abstract

Background

It is suggested that hypoxic–ischemic retinal diseases induce loss of retinal ganglion cells. Excess glutamate release is involved in these conditions. A predominant function of Müller cells is to regulate glutamate levels, but in these diseases the function is compromised. The present study was performed to investigate the role of interleukin-1β(IL-1β)on the glutamate uptake in retinal Müller cells under hypoxia and to study the possible mechanism.

Methods

The levels of IL-1β,Kir4.1, and GLAST in retinal Müller cells under hypoxia were analyzed by Western blotting and realtime-RT-PCR, and glutamate uptake assay was undertaken to investigate the activity of GLAST. After being treated with IL-1βunder normoxia, these proteins (Kir4.1 and GLAST) and their mRNAs, and glutamate uptake activity in Müller cells were investigated. To confirm the effect of IL-1βon glutamate uptake activity in Müller cells, addition of IL-1ra was used.

Results

Under hypoxia, Müller cells glutamate uptake, Kir4.1 and GLAST expressions were decreased significantly; however, IL-1βexpression was increased. IL-1βtreatment induced depression of glutamate uptake, decrease of Kir4.1 and GLAST expressions in retinal Müller cells under normoxia. Moreover, addition of IL-1ra significantly ameliorated decreases in Kir4.1 and GLAST expressions, and compromise of glutamate uptake activity in retinal Müller cells under hypoxia.

Conclusions

These findings indicated that decreases in Kir4.1 and GLAST expressions and depression of glutamate uptake in retinal Müller cells under hypoxia may be induced by the inflammatory cytokine IL-1β.

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ACKNOWLEDGMENTS

This study was supported by the National Nature Science Foundation of China (81170860), Shanghai Nature Science Foundation (11ZR1422000) and Natural Science Foundation of Ningbo City (2012A610216).

Declaration of interest: The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.

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Authors and Affiliations

  1. Department of Ophthalmology, The Affiliated Cixi Hospital of Wenzhou Medical University, 315300, Cixi, Zhejiang, China

    Chongda Chen, Hui Chen & Chunliu Xu

  2. Department of Ophthalmology, Ruijin Hospital , School of Medicine, The Jiaotong University, 200025, Shanghai, China

    Yisheng Zhong & Xi Shen

  3. Department of Ophthalmology, Ruijin Hospital , LuWan Branch, School of Medicine, The Jiaotong University, 200025, Shanghai, China

    Xi Shen

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  1. Chongda Chen
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  2. Hui Chen
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Correspondence to Yisheng Zhong or Xi Shen.

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Chen, C., Chen, H., Xu, C. et al. Role of interleukin-1β in hypoxia-induced depression of glutamate uptake in retinal Müller cells. Graefes Arch Clin Exp Ophthalmol 252, 51–58 (2014). https://doi.org/10.1007/s00417-013-2516-z

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  • DOI: https://doi.org/10.1007/s00417-013-2516-z

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