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Phylogenetic analyses of the 19 STR loci in X chromosome revealed discriminant forensic characteristics for Chinese Daur and Oroqen minorities

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Abstract

In respect of forensic genetics, X-STRs are widely applied for deficiency paternity cases. Given the popularization of AGCU-X19 STR Kit in China, there has been investigation conducted into the genetic data and forensic parameters of 19 X-STR loci in many of the Chinese ethnic groups, which makes it possible to perform nationwide phylogenetic comparation. To evaluate the allele and haplotype diversity of 19 X-STR loci and to explore their forensic efficiency in the Daur and Oroqen minorities, unrelated healthy Daur (n = 86) and Oroqen (n = 165) individuals were recruited from Heilongjiang province, so as to reveal the phylogenetic relationship between the two minorities and other Chinese ethnic groups. Of the Daur and Oroqen minorities, 172 and 183 alleles at the 19 X-STR loci were observed, respectively. Haplotype diversity exceeded 0.9 among all the linkage clusters. High cumulative value was observed for the power of discrimination, the probability of exclusion, and the mean exclusion chance for deficiency cases (normal trios and duo cases). As revealed by this study, the panel of 19 X-STR loci is an effective supplementary tool for the kinship test of the studied nationalities.

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Acknowledgements

The authors extend gratitude to the voluntary participants in this study.

Funding

This study was funded by opening project of the Shanghai Key Laboratory of Forensic Medicine [grant number KF1909] and the 2017 Research Program of Shaoxing University (2017LG1009).

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Author notes

  1. Yi Ye contributed equally to this work and should be considered co-first author.

Authors and Affiliations

  1. Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin, 150025, Heilongjiang, China

    Yang Liu & Dong-Yun Hao

  2. Forensic Center, College of Medicine, Shaoxing University, No. 508 Huancheng West Road, Shaoxing, 312000, Zhejiang, China

    Yang Liu & Guang-Yao Fan

  3. Department of Forensic Toxicological Analysis, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China

    Yi Ye & Xiao-Qin Yi

  4. College of Medicine, Shaoxing University, No. 508 Huancheng West Road, Shaoxing, 312000, Zhejiang, China

    Jia-Hui Zhang

Authors
  1. Yang Liu
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  2. Yi Ye
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  3. Xiao-Qin Yi
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  4. Jia-Hui Zhang
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  5. Guang-Yao Fan
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Corresponding authors

Correspondence to Guang-Yao Fan or Dong-Yun Hao.

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We have obtained expressed consent from all volunteers before sampling. This study was approved by the Ethics Committee of the Medical College, Shaoxing University (No. 2020–001).

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The authors declare no competing interests.

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Liu, Y., Ye, Y., Yi, XQ. et al. Phylogenetic analyses of the 19 STR loci in X chromosome revealed discriminant forensic characteristics for Chinese Daur and Oroqen minorities. Int J Legal Med 136, 551–553 (2022). https://doi.org/10.1007/s00414-021-02697-7

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  • DOI: https://doi.org/10.1007/s00414-021-02697-7

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