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Analytical validation of an RI sample cartridge with the RapidHIT® ID system

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Abstract

Evaluating the short tandem repeat (STR) in the field is important for the timely identification of a suspect. Several lines showed that the RapidHIT® ID system is reliable for DNA genotyping with buccal swabs and naked DNA. However, the application of this approach with blood samples has been poorly investigated. Because blood samples are among the most common forensic samples in our laboratory, further studies should be conducted. Here, we assessed the analytical performance of 19 STR loci with a newly developed RapidINTEL (RI) Sample Cartridge Kit by using the blood samples with known genotypes. Several commonly used substrates were included in the sensitivity study, and FTA cards proved to be the most promising sample carrier for blood storage and later identification. There was superior sensitivity and specificity with a 100% concordance rate for 0.5 μL of blood or 7 ng of genomic DNA. The performance for blood samples was comparable with that for the standard protocol. High success rate (90.57%) and high-concordance (100%) genotyping were automatically achieved over a wide range of operating conditions except for TH01. No contamination was observed throughout the study. Hematin, indigo, and humic acid had limited influence on the instrument system, while urea and melanin dramatically affected the genotyping results. Generally, the newly developed RI sample cartridge provided an alternative method for the STR genotyping of single-source blood samples over a wide range of operating conditions.

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References

  1. Dumache R, Ciocan V, Muresan C, Enache A (2016) Molecular DNA analysis in forensic identification. Clin Lab 62(1–2):245–248. https://doi.org/10.7754/clin.lab.2015.150414

    Article  CAS  PubMed  Google Scholar 

  2. Morrison J, Watts G, Hobbs G, Dawnay N (2018) Field-based detection of biological samples for forensic analysis: established techniques, novel tools, and future innovations. Forensic SciInt 285:147–160. https://doi.org/10.1016/j.forsciint.2018.02.002

    Article  CAS  Google Scholar 

  3. Jovanovich S, Bogdan G, Belcinski R, Buscaino J, Burgi D, Butts ELR, Chear K, Ciopyk B, Eberhart D, El-Sissi O, Franklin H, Gangano S, Gass J, Harris D, Hennessy L, Kindwall A, King D, Klevenberg J, Li Y, Mehendale N, McIntosh R, Nielsen B, Park C, Pearson F, Schueren R, Stainton N, Troup C, Vallone PM, Vangbo M, Woudenberg T, Wyrick D, Williams S (2015) Developmental validation of a fully integrated sample-to-profile rapid human identification system for processing single-source reference buccal samples. Forensic SciInt Genet 16:181–194. https://doi.org/10.1016/j.fsigen.2014.12.004

    Article  CAS  Google Scholar 

  4. Hennessy LK, Mehendale N, Chear K, Jovanovich S, Williams S, Park C, Gangano S (2014) Developmental validation of the GlobalFiler(®) express kit, a 24-marker STR assay, on the RapidHIT(®) System. Forensic SciInt Genet 13:247–258. https://doi.org/10.1016/j.fsigen.2014.08.011

    Article  CAS  Google Scholar 

  5. LaRue BL, Moore A, King JL, Marshall PL, Budowle B (2014) An evaluation of the RapidHIT(®) system for reliably genotyping reference samples. Forensic SciInt Genet 13:104–111. https://doi.org/10.1016/j.fsigen.2014.06.012

    Article  CAS  Google Scholar 

  6. Salceda S, Barican A, Buscaino J, Goldman B, Klevenberg J, Kuhn M, Lehto D, Lin F, Nguyen P, Park C, Pearson F, Pittaro R, Salodkar S, Schueren R, Smith C, Troup C, Tsou D, Vangbo M, Wunderle J, King D (2017) Validation of a rapid DNA process with the RapidHIT(®) ID system using GlobalFiler(®) Express chemistry, a platform optimized for decentralized testing environments. Forensic SciInt Genet 28:21–34. https://doi.org/10.1016/j.fsigen.2017.01.005

    Article  CAS  Google Scholar 

  7. Shackleton D, Gray N, Ives L, Malsom S, Vanhinsbergh D (2019) Development of RapidHIT(®) ID using NGMSElectTM Express chemistry for the processing of reference samples within the UK criminal justice system. Forensic SciInt 295:179–188. https://doi.org/10.1016/j.forsciint.2018.12.015

    Article  CAS  Google Scholar 

  8. Amick GD, Swiger RR (2019) Internal validation of RapidHIT(®) ID ACE sample cartridge and assessment of the EXT sample cartridge*(†). J Forensic Sci 64(3):857–868. https://doi.org/10.1111/1556-4029.13921

    Article  CAS  PubMed  Google Scholar 

  9. SWGDAM (n.d.) SWGDAM validation guidelines for DNA analysis methods (Approved 12/05/2016). Available at http://www.swgdam.org/

  10. Zheng H, Tao R, Zhang J, Zhang J, Wang S, Yang Z, Xu Q, Gao Y, Zhang S, Li C (2019) Development and validation of a novel SiFaSTR(TM) 23-plex system. Electrophoresis 40(20):2644–2654. https://doi.org/10.1002/elps.201900045

    Article  CAS  PubMed  Google Scholar 

  11. Verdon TJ, Mitchell RJ, van Oorschot RA (2013) The influence of substrate on DNA transfer and extraction efficiency. Forensic SciInt Genet 7(1):167–175. https://doi.org/10.1016/j.fsigen.2012.09.004

    Article  CAS  Google Scholar 

  12. Farash K, Hanson EK, Ballantyne J (2015) Enhanced genetic analysis of single human bioparticles recovered by simplified micromanipulation from forensic 'touch DNA' evidence. J Visualized Exp 97. https://doi.org/10.3791/52612

  13. Peng F, Feng L, Chen J, Wang L, Li P, Ji A, Zeng C, Liu F, Li C (2019) Validation of methylation-based forensic age estimation in time-series bloodstains on FTA cards and gauze at room temperature conditions. Forensic SciInt Genet 40:168–174. https://doi.org/10.1016/j.fsigen.2019.03.006

    Article  CAS  Google Scholar 

  14. Rahikainen AL, Palo JU, de Leeuw W, Budowle B, Sajantila A (2016) DNA quality and quantity from up to 16 years old post-mortem blood stored on FTA cards. Forensic SciInt 261:148–153. https://doi.org/10.1016/j.forsciint.2016.02.014

    Article  CAS  Google Scholar 

  15. Green H, Tillmar A, Pettersson G, Montelius K (2019) The use of FTA cards to acquire DNA profiles from postmortem cases. Int J Legal Med 133(6):1651–1657. https://doi.org/10.1007/s00414-019-02015-2

    Article  PubMed  Google Scholar 

  16. Dong L, Lin C, Li L, Wang M, Cui J, Feng R, Liu B, Wu Z, Lian J, Liao G, Chen W, Qiao Y (2017) An evaluation of clinical performance of FTA cards for HPV 16/18 detection using cobas 4800 HPV Test compared to dry swab and liquid medium. J ClinVirol 94:67–71. https://doi.org/10.1016/j.jcv.2017.06.008

    Article  CAS  Google Scholar 

  17. Qin Y, Zhang H, Marlowe N, Fei M, Yu J, Lei X, Yu L, Zhang J, Cao D, Ma L, Chen W (2016) Evaluation of human papillomavirus detection by Abbott m2000 system on samples collected by FTA EluteTM Card in a Chinese HIV-1 positive population. J ClinVirol 85:80–85. https://doi.org/10.1016/j.jcv.2016.11.002

    Article  CAS  Google Scholar 

  18. Hashimoto M, Bando M, Kido JI, Yokota K, Mita T, Kajimoto K, Kataoka M (2019) Nucleic acid purification from dried blood spot on FTA Elute Card provides template for polymerase chain reaction for highly sensitive Plasmodium detection. ParasitolInt 73:101941. https://doi.org/10.1016/j.parint.2019.101941

    Article  CAS  Google Scholar 

  19. Lalani T, Tisdale MD, Liu J, Mitra I, Philip C, Odundo E, Reyes F, Simons MP, Fraser JA, Hutley E, Connor P, Swierczewski BE, Houpt E, Tribble DR, Riddle MS (2018) Comparison of stool collection and storage on Whatman FTA Elute cards versus frozen stool for enteropathogen detection using the TaqMan Array Card PCR assay. PLoS ONE 13(8):e0202178. https://doi.org/10.1371/journal.pone.0202178

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Buscaino J, Barican A, Farrales L, Goldman B, Klevenberg J, Kuhn M, Lin F, Nguyen P, Salceda S, Schueren R, Smith C, Troup C, Tsou D, Vangbo M, King D (2018) Evaluation of a rapid DNA process with the RapidHIT(®) ID system using a specialized cartridge for extracted and quantified human DNA. Forensic SciInt Genet 34:116–127. https://doi.org/10.1016/j.fsigen.2018.02.010

    Article  CAS  Google Scholar 

  21. Date-Chong M, Hudlow WR, Buoncristiani MR (2016) Evaluation of the RapidHITTM 200 and RapidHITGlobalFiler(®) Express kit for fully automated STR genotyping. Forensic SciInt Genet 23:1–8. https://doi.org/10.1016/j.fsigen.2016.03.001

    Article  CAS  Google Scholar 

  22. Wiley R, Sage K, LaRue B, Budowle B (2017) Internal validation of the RapidHIT(®) ID system. Forensic SciInt Genet 31:180–188. https://doi.org/10.1016/j.fsigen.2017.09.011

    Article  CAS  Google Scholar 

  23. Tao R, Qi W, Chen C, Zhang J, Yang Z, Song W, Zhang S, Li C (2019) Pilot study for forensic evaluations of the Precision ID GlobalFilerTM NGS STR Panel v2 with the Ion S5TM system. Forensic SciInt Genet 43:102147. https://doi.org/10.1016/j.fsigen.2019.102147

    Article  CAS  Google Scholar 

  24. Elwick K, Zeng X, King J, Budowle B, Hughes-Stamm S (2018) Comparative tolerance of two massively parallel sequencing systems to common PCR inhibitors. Int J Legal Med 132(4):983–995. https://doi.org/10.1007/s00414-017-1693-4

    Article  PubMed  Google Scholar 

  25. Meng H, Guo Y, Jin X, Chen C, Cui W, Shi J, Wang X, Liu R, Zhu B (2019) Internal validation study of a newly developed 24-plex Y-STRs genotyping system for forensic application. Int J Legal Med 133(3):733–743. https://doi.org/10.1007/s00414-019-02028-x

    Article  PubMed  Google Scholar 

  26. Hu N, Cong B, Li S, Ma C, Fu L, Zhang X (2014) Current developments in forensic interpretation of mixed DNA samples (Review). Biomed Rep 2(3):309–316. https://doi.org/10.3892/br.2014.232

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Biedermann A, Bozza S, Konis K, Taroni F (2012) Inference about the number of contributors to a DNA mixture: Comparative analyses of a Bayesian network approach and the maximum allele count method. Forensic SciInt Genet 6(6):689–696. https://doi.org/10.1016/j.fsigen.2012.03.006

    Article  CAS  Google Scholar 

  28. Liu C, Harashima N, Katsuyama Y, Ota M, Arakura A, Fukushima H (1997) ACTBP2 gene frequency distribution and sequencing of the allelic ladder and variants in the Japanese and Chinese populations. Int J Legal Med 110(4):208–212. https://doi.org/10.1007/s004140050069

    Article  CAS  PubMed  Google Scholar 

  29. Yuan GL, Shen CM, Wang HD, Liu WJ, Yang G, Yan JW, Qin HX, Xie T, Ran H, Yuan J, Liu Z, Zhu B (2012) Genetic data provided by 21 autosomal STR loci from Chinese Tujia ethnic group. Mol Biol Rep 39(12):10265–10271. https://doi.org/10.1007/s11033-012-1903-6

    Article  CAS  PubMed  Google Scholar 

  30. Chen L, Lu HJ, Du WA, Qiu PM, Liu C (2016) Polymorphism analysis of 20 autosomal short-tandem repeat loci in southern Chinese Han population. Nan Fang Yi Ke Da XueXue Bao 37(2):141–149. https://doi.org/10.3969/j.issn.1673-4254.2017.02.01

    Article  Google Scholar 

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Funding

This study was supported by grants from the National Natural Science Fund of China (Nos. 81625013 and 81772028), the Shanghai Science and Technology Innovation Fund (19DZ2201400), the Shanghai Outstanding Academic Leaders Plan (2017485).

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Authors and Affiliations

  1. Department of Forensic Medicine, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, China

    Anqi Chen & Chengtao Li

  2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Ministry of Justice, Academy of Forensic Science, Shanghai, 200063, China

    Anqi Chen, Yue Yang, Qi Yang, Ruiyang Tao, Chong Chen, Suhua Zhang & Chengtao Li

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  1. Anqi Chen
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  2. Yue Yang
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  3. Qi Yang
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  6. Suhua Zhang
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  7. Chengtao Li
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Correspondence to Suhua Zhang or Chengtao Li.

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The study was approved by the Ethics Committee of Academy of Forensic Science, Ministry of Justice, China. All participates provided written informed consent.

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Chen, A., Yang, Y., Yang, Q. et al. Analytical validation of an RI sample cartridge with the RapidHIT® ID system. Int J Legal Med 135, 1257–1265 (2021). https://doi.org/10.1007/s00414-021-02553-8

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  • DOI: https://doi.org/10.1007/s00414-021-02553-8

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