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Genetic diversity and haplotype analysis of Guizhou Miao identified with 19 X-chromosomal short tandem repeats

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Abstract

We analyzed Chinese Miao population samples (n = 268) from Guizhou province, Southwest China, with 19 X-chromosomal short tandem repeats (STRs) included in the AGCU X19 amplification kit. The combined PE is 0.999999922 and the combined PDs in males and females are 0.9999999999999999999994 and 0.9999999999998, respectively. The mean paternity exclusion change values are larger than 0.99999996. The HDs in seven linkage groups vary from 0.9324 to 0.9968. Subsequently, comparison among different Chinese populations shows that the substructures of Chinese were significantly influenced by ethno-linguistic rather than geographical boundaries, including most prominently Turkic-speaking and Tibeto-Burman-speaking populations.

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References

  1. Szibor R (2007) X-chromosomal markers: past, present and future. Forensic Sci Int Genet 1:93–99. https://doi.org/10.1016/j.fsigen.2007.03.003

    Article  PubMed  Google Scholar 

  2. Wen B, Li H, Gao S, Mao X, Gao Y, Li F, Zhang F, He Y, Dong Y, Zhang Y, Huang W, Jin J, Xiao C, Lu D, Chakraborty R, Su B, Deka R, Jin L (2005) Genetic structure of Hmong-Mien speaking populations in East Asia as revealed by mtDNA lineages. Mol Biol Evol 22:725–734. https://doi.org/10.1093/molbev/msi055

    Article  CAS  PubMed  Google Scholar 

  3. He G, Li Y, Zou X, Li P, Chen P, Song F, Gao T, Liao M, Yan J, Wu J (2017) Forensic characteristics and phylogenetic analyses of the Chinese Yi population via 19 X-chromosomal STR loci. Int J Legal Med 131:1243–1246. https://doi.org/10.1007/s00414-017-1563-0

    Article  PubMed  Google Scholar 

  4. He G, Li Y, Zou X, Wang M, Chen P, Liao M, Wu J (2017) Genetic polymorphisms for 19 X-STR loci of Sichuan Han ethnicity and its comparison with Chinese populations. Leg Med (Tokyo) 29:6–12. https://doi.org/10.1016/j.legalmed.2017.09.001

    Article  CAS  Google Scholar 

  5. He G, Li Y, Zou X, Zhang Y, Li H, Wang M, Wu J (2018) X-chromosomal STR-based genetic structure of Sichuan Tibetan minority ethnicity group and its relationships to various groups. Int J Legal Med 132:409–413. https://doi.org/10.1007/s00414-017-1672-9

    Article  PubMed  Google Scholar 

  6. Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16:1215

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Excoffier L, Lischer HE (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567. https://doi.org/10.1111/j.1755-0998.2010.02847.x

    Article  PubMed  Google Scholar 

  8. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874. https://doi.org/10.1093/molbev/msw054

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Funding

This study received the support of grants from the National Natural Science Foundation of China (No. 81401562), the Fundamental Research Funds for the Central University (2012017yjsy187), the Science and Technology Foundation of Guizhou Province (No. 2013-2313), and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (No. 2014-1685).

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Author notes

  1. Yanyan Han and Guanglin He contributed equally to this work.

Authors and Affiliations

  1. School of Public Health, Zunyi Medical University, Zunyi, 563099, Guizhou, China

    Yanyan Han & Zhigang Jiang

  2. Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China

    Guanglin He

  3. Criminal Investigation Detachment, Qianxinan Prefecture Public Security Bureau, Xingyi, Guizhou, China

    Sheng Gong

  4. Judicial Expertise Center, Zunyi City Public Security Bureau, Zunyi, 563099, Guizhou, China

    Junying Chen

  5. Center of Forensic Expertise, Affiliated Hospital of Zunyi Medical University, Zunyi, 563099, Guizhou, China

    Pengyu Chen

  6. School of Forensic Medicine, Zunyi Medical University, Zunyi, 563099, Guizhou, China

    Pengyu Chen

Authors
  1. Yanyan Han
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  2. Guanglin He
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  3. Sheng Gong
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  4. Junying Chen
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  5. Zhigang Jiang
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  6. Pengyu Chen
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Corresponding author

Correspondence to Pengyu Chen.

Ethics declarations

This study had been approved by the institutional review boards at Zunyi Medical University. Informed consent was obtained from volunteers before sample collection.

Competing interests

The authors declare that they have no competing interests.

Electronic supplementary material

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Figure S1

Genetic affinity and differences between the Guizhou Miao and 12 Chinese reference populations based on the genetic variants of 19 X-STRs. (A) Principle component analysis was done by the genetic variances of PCA1 and PCA2. Multidimensional Scaling Plots (B) and Phylogenetic Neighbor-Joining tree (C) were constructed based on Nei’s genetic distances matrix. (PNG 470 kb)

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Han, Y., He, G., Gong, S. et al. Genetic diversity and haplotype analysis of Guizhou Miao identified with 19 X-chromosomal short tandem repeats. Int J Legal Med 133, 99–101 (2019). https://doi.org/10.1007/s00414-018-1871-z

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  • DOI: https://doi.org/10.1007/s00414-018-1871-z

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