Abstract
Study results of variations in the X chromosome are useful tools in researching the genetic diversity of human populations and individual identification. We developed a 13 X chromosomal short tandem repeat (STR) multiplex system (DXS6807, DXS8378, DSX9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7424, DXS101, GATA172D05, HPRTB, DXS8377, DXS7423) amplified in one single polymerase chain reaction. DNA samples of 113 male and 108 female Taiwanese Han subjects were successfully analyzed using this 13 X-STR multiplex system. The distributions of allele frequencies were examined for independence. DXS8377, DXS101, DXS6789, and DXS6809 were found to be the most polymorphic markers in this study. High values of discrimination power and mean exclusion chance without significant evidence of association between these markers were obtained. In conclusion, this 13 X chromosomal STR multiplex system offers considerable forensic efficiency and may be useful in forensic identification casework.
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References
Ross MT, Grafham DV, Coffey AJ et al (2005) The DNA sequence of the human X chromosome. Nature 434:325–337
Sachidanandam R, Weissman D, Schmidt SC et al (2001) A map of human genome sequence variation containing 1.42 million single nucleotide polymorphisms. Nature 409:928–933
Edelmann J, Deichsel D, Hering S, Plate I, Szibor R (2002) Sequence variation and allele nomenclature for the X-linked STRs DXS9895, DXS8378, DXS7132, DXS6800, DXS7133, GATA172D05, DXS7423 and DXS8377. Forensic Sci Int 129:99–103
Zarrabeitia MT, Amigo T, Sanudo C, Zarrabeitia A, González-Lamuño D, Riancho JA (2002) A new pentaplex system to study short tandem repeat markers of forensic interest on X chromosome. Forensic Sci Int 129:85–89
Athanasiadou D, Stradmann-Bellinghausen B, Rittner C, Alt KW, Schneider PM (2003) Development of a quadruplex PCR system for the genetic analysis of X-chromosomal STR loci. Int Congr Ser 1239:311–314
Bini C, Ceccardi S, Ferri G et al (2005) Development of a heptaplex PCR system to analyse X-chromosome STR loci from five Italian population samples. A collaborative study. Forensic Sci Int 153:231–236
Poetsch M, Petersmann H, Repenning A, Lignitz E (2005) Development of two pentaplex systems with X-chromosomal STR loci and their allele frequencies in a northeast German population. Forensic Sci Int 155:71–76
Tabbada KA, De Ungria MCA, Faustino LP, Athanasiadou D, Stradmann-Bellinghausen B, Schneider PM (2005) Development of a pentaplex X-chromosomal short tandem repeat typing system and population genetic studies. Forensic Sci Int 154:173–180
Asamura H, Sakai H, Kobayashi K, Ota M, Fukushima H (2006) MiniX-STR multiplex system population study in Japan and application to degraded DNA analysis. Int J Legal Med 120:174–181
Robino C, Giolitti A, Gino S, Torre C (2006) Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample. Int J Legal Med 120:315–318
Gomes I, Prinz M, Pereira R et al (2007) Genetic analysis of three US population groups using an X-chromosomal STR decaplex. Int J Legal Med 121:198–203
Pereira R, Gomes I, Amorim A, Gusmão L (2007) Genetic diversity of 10 X chromosome STRs in northern Portugal. Int J Legal Med 121:192–197
Sullivan KM, Mannucci A, Kimpton PA, Gill P (1993) A rapid and quantitative DNA sex test: fluorescence-based PCR analysis of X-Y homologous gene amelogenin. Biotechniques 15:636–641
Raymond M, Rousset F (1995) GENEPOP (version 1.2): a population genetics software for exact tests and eucumenicism. J Hered 86:248–249 (website: http://www.cefe.cnrs-mop.fr/)
Szibor R, Edelmann J, Hering S et al (2003) Cell line DNA typing in forensic genetics—the necessity of reliable standards. Forensic Sci Int 138:37–43
Fracasso T, Schürenkamp M, Brinkmann B, Hohoff C (2008) An X-STR meiosis study in Kurds and Germans: allele frequencies and mutation rates. Int J Legal Med 122:353–356
Shin SH, Yu JS, Park SW, Min GS, Chung KW (2005) Genetic analysis of 18 X-linked short tandem repeat markers in Korean population. Forensic Sci Int 147:35–41
Pico A, Castillo A, Vargas C, Amorim A, Gusmão L (2008) Genetic profile characterization and segregation analysis of 10 X-STRs in a sample from Santander, Colombia. Int J Legal Med 122:347–351
Szibor R, Hering S, Kuhlisch E, Plate I, Demberger S, Krawczak M, Edelmann J (2005) Haplotyping of STR cluster DXS6801–DXS6809–DXS6789 on Xq21 provides a powerful tool for kinship testing. Int J Legal Med 119:363–369
Edelmann J, Hering S, Kuhlisch E, Szibor R (2002) Validation of the STR DXS7424 and the linkage situation on the X-chromosome. Forensic Sci Int 125:217–222
Acknowledgements
The authors thank Ms Hong-Ciao Wan, Ms Pi-Mei Hsu, Ms Shwu-Fang Li, Ms Yi-Li Liu and Ms I-Chin Huang for technical support on DNA extraction and DNA sequencing. This work was partially supported by a grant from the Department of Medical Research at National Taiwan University Hospital.
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Hwa, HL., Chang, YY., Lee, J.CI. et al. Thirteen X-chromosomal short tandem repeat loci multiplex data from Taiwanese. Int J Legal Med 123, 263–269 (2009). https://doi.org/10.1007/s00414-008-0310-y
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DOI: https://doi.org/10.1007/s00414-008-0310-y