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The STR cluster DXS10148–DXS8378–DXS10135 provides a powerful tool for X-chromosomal haplotyping at Xp22

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Abstract

The evaluation of four pairs of tightly linked chromosome X (ChrX) short tandem repeat (STR)s at Xp22, Xq12, Xq26 and Xq28 led to the creation of the Argus X 8 multiplex amplification kit. These eight STRs are distributed as four closely linked pairs over the entire X-chromosome, and for practical reasons, they are assigned to four linkage groups 1–4. To achieve a further considerable enhancement in discrimination power, we suggest to include additional markers. A recent paper referred to the earlier evaluation of STR clusters at Xq12, Xq26 and Xq28, and here we present the pending data of linkage group 1 at Xp22. The newly established STR updates the Xp22 STR cluster which now presents three polymorphic markers: DXS10148 (PIC = 0.8556), DXS10135 (PIC = 0.9093) and DXS 8378 (PIC = 0.6454). Typing of 398 X-chromosomes provided 278 different and 200 unique haplotypes. All the other haplotypes observed appeared with frequencies in the range between 0.005 and 0.015. Considering this STR triple in the context with the three further triple clusters Xq12, Xq26 and Xq28 published earlier, we announced the development of a next generation of a ChrX STR cluster typing kit.

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Authors and Affiliations

  1. Institut für Rechtsmedizin, Otto-von-Guericke-Universität Magdeburg, Leipziger Straße 44, 39120, Magdeburg, Germany

    Tanja Hundertmark & Reinhard Szibor

  2. Institut für Rechtsmedizin, Universität Leipzig, Johannisallee 28, 04103, Leipzig, Germany

    Jeanett Edelmann

  3. Gerichtsmedizin Magdeburg, Domplatz 11, 39104, Magdeburg, Germany

    Ines Plate

  4. Institut für Rechtsmedizin, Universitätsklinikum Hamburg-Eppendorf, Butenfeld 34, 22529, Hamburg, Germany

    Christa Augustin

  5. Institut für Rechtsmedizin, Technische Universität Dresden, Fetscherstrasse 74, 01307, Dresden, Germany

    Sandra Hering

Authors
  1. Tanja Hundertmark
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  2. Sandra Hering
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  3. Jeanett Edelmann
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  4. Christa Augustin
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  5. Ines Plate
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  6. Reinhard Szibor
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Corresponding author

Correspondence to Reinhard Szibor.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

1Xp22 STRs: Primer sequences and positions (DOC 37.5 KB)

ESM 2

DXS10148, DXS10135 and DXS8378: allele nomenclature, amplicon length and repeat composition primer positions are underlined; ** primer 1 covers 2.2 repeats; A* is present only in the alleles 21.1, 22.1 and 23.1–30.1 and absent in alleles 18–21, 22 and 23. (DOC 19.5 KB)

ESM 3

DXS8378–DXS10135–DXS19145 haplotype frequencies in a sample of 398 chromosomes (DOC 498 KB)

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Hundertmark, T., Hering, S., Edelmann, J. et al. The STR cluster DXS10148–DXS8378–DXS10135 provides a powerful tool for X-chromosomal haplotyping at Xp22. Int J Legal Med 122, 489–492 (2008). https://doi.org/10.1007/s00414-008-0277-8

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  • DOI: https://doi.org/10.1007/s00414-008-0277-8

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