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Amelogenin sex determination by pyrosequencing of short PCR products

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Abstract

We developed an assay, which allows the sex determination of human DNA samples by pyrosequencing of short PCR products. A 48/45-bp stretch including primers of the amelogenin gene with a 3-bp insertion on the Y chromosome was chosen for analysis. In an initial study, we correctly typed 50 male and 50 female DNA samples from unrelated donors. First experiments with forensic samples, which failed in conventional analyses, indicate that this approach might be an advantage when dealing with degraded DNA.

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References

  1. Kashyap VK, Sahoo S, Sitalaximi T, Trivedi R (2006) Deletions in the Y-derived amelogenin gene fragment in the Indian population. BMJ Med Genet 7:37

    Article  CAS  Google Scholar 

  2. Steinlechner M, Berger B, Niederstätter H, Parson W (2002) Rare failures in the amelogenin sex test. Int J Legal Med 116:117–120

    Article  PubMed  CAS  Google Scholar 

  3. Santacroce R, Vecchione G, Tomaiyolo M, Sessa F, Sarno M, Colaizzo D, Grandone E, Margaglione M (2006) Identification of fetal gender in maternal blood is a helpful tool in the prenatal diagnosis of haemophilia. Haemophilia 12(4):417–422

    Article  PubMed  CAS  Google Scholar 

  4. Sullivan KM, Mannucci A, Kimpton CP, Gill P (1993) A rapid and quantitative DNA sex test: fluorescence-based PCR analysis of X–Y homologous gene amelogenin. Biotechniques 15:636–641

    PubMed  CAS  Google Scholar 

  5. Ahmadian A, Ehn M, Hober S (2006) Pyrosequencing: history, biochemistry and future. Clin Chim Acta 363(1–2):83–94

    Article  PubMed  CAS  Google Scholar 

  6. Balitzki-Korte B, Anslinger K, Bartsch C, Rolf B (2005) Species identification by means of pyrosequencing the mitochondrial 12S rRNA gene. Int J Legal Med 119:291–294

    Article  PubMed  CAS  Google Scholar 

  7. Andrèasson H, Nilsson M, Budowle B, Frisk S, Allen M (2006) Quantification of mtDNA mixtures in forensic evidence material using pyrosequencing. Int J Legal Med 120:383–390

    Article  PubMed  Google Scholar 

  8. Frey UH, Nuckel H, Dobrev D, Manthey I, Sandalcioglu IE, Eisenhardt A, Worm K, Hauner H, Siffert W (2005) Quantification of G protein Gaalphas subunit splice variants in different human tissues and cells using pyrosequencing. Gene Expr 12/2:69–81

    Article  Google Scholar 

  9. Sun A, Ge J, Siffert W, Frey UH (2005) Quantification of allele-specific G-protein b3 subunit mRNA transcripts in different human cells and tissues by pyrosequencing. Eur J Hum Genet 13:361–369

    Article  PubMed  CAS  Google Scholar 

  10. Brinkmann B (2002) Is the amelogenin sex test valid? Int J Legal Med 116:63

    Article  PubMed  CAS  Google Scholar 

  11. Gusmao L, Butler JM, Carracedo A, Gill P, Kayser M, Mayr WR, Morling N, Prinz M, Roewer L, Tyler-Smith C, Schneider PM, International Society of Forensic Genetics (2006) DNA Commission of the International Society of Forensic Genetics (ISFG): an update of the recommendations on the use of Y-STRs in forensic analysis. Int J Legal Med 120(4):191–200

    Article  PubMed  CAS  Google Scholar 

  12. Santos FR, Pandya A, Tyler-Smith C (1998) Reliability of DNA-based sex tests. Nat Genet 18:103

    Article  PubMed  CAS  Google Scholar 

  13. Thangaraj K, Reddy AG, Singh L (2002) Is the amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis? Int J Legal Med 116:121–123

    Article  PubMed  CAS  Google Scholar 

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Author notes

  1. Frank Tschentscher

    Present address: Landeskriminalamt NRW, Kriminaltechnisches Institut, Düsseldorf, Germany

Authors and Affiliations

  1. Institute of Legal Medicine, University of Duisburg-Essen, Essen, Germany

    Frank Tschentscher & Thomas Bajanowski

  2. Institute of Pharmacogenetics, University of Duisburg-Essen, Essen, Germany

    Ulrich H. Frey

  3. Department of Anaesthesiology, University of Duisburg-Essen, Essen, Germany

    Ulrich H. Frey

  4. Institute of Legal Medicine, Hufelandstr. 55, 5122, Essen, Germany

    Thomas Bajanowski

Authors
  1. Frank Tschentscher
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  2. Ulrich H. Frey
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  3. Thomas Bajanowski
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Corresponding author

Correspondence to Thomas Bajanowski.

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Tschentscher, F., Frey, U.H. & Bajanowski, T. Amelogenin sex determination by pyrosequencing of short PCR products. Int J Legal Med 122, 333–335 (2008). https://doi.org/10.1007/s00414-008-0228-4

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  • DOI: https://doi.org/10.1007/s00414-008-0228-4

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