Abstract
The aim of this study was to develop a new method for species identification based on the analysis of a very short nucleotide sequence. For this reason, the mitochondrial 12S rRNA gene, together with the new method of pyrosequencing, was used. The detection of only 20 nucleotides, following the sequencing primer within a 149-bp fragment by pyrosequencing, was sufficient to identify the biological origin of the samples by alignment with a reference sequence database. A case example with a piece of skin is presented, and the question whether this piece of skin came from a missing wife or from an animal could be answered.
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References
Anderson S, Bankier AT, Barrell BG et al (1981) Sequence and organisation of the human mitochondrial genome. Nature 290:457–464
Anslinger K, Weichhold G, Keil W, Bayer B, Eisenmenger W (2001) Identification of the skeletal remains of Martin Bormann by mtDNA analysis. Int J Leg Med 114:194–196
Austin JJ, Arnold EN, Jones CG (2004) Reconstructing an island radiation using ancient and recent DNA: the extinct and living day geckos (phelsuma) of the Mascarene Islands. Mol Phylogenet Evol 31:109–122
Beati L, Caceres AG, Lee JA, Munstermann LE (2004) Systematic relationship among Lutzomyia sand flies (diptera: psychodidae) of Peru and Colombia based on the analysis of 12S and 28S ribosomal DNA sequence. Int J Parasitol 34:225–234
Chikune K, Tabata T, Saito M, Monma M (1994) Sequencing of mitochondrial cytochrome b genes for the identification of meat species. Anim Sci Technol 65:571–579
Forrest AAR, Carnegie PR (1994) Identification of gourmet meat using FINS (forensically informative nucleotide sequencing). Biotechniques 17:24–26
Noller HF (1984) Structure of ribosomal RNA. Ann Rev Biochem 53:119–162, (253)
Parson W, Pegoraro K, Niederstätter H, Föger M, Steinlechner M (2000) Species identification by means of the cytochrome b gene. Int J Leg Med 114:23–28
Pfeiffer H, Benthaus S, Rolf B, Brinkmann B (2003) The Kaiser’s tooth. Int J Leg Med 117:118–120
Ronaghi M (2001) Pyrosequencing sheds light on DNA sequencing. Genome Res 11:3–11
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci U S A 74:5463
Smith RF, Wiese BA, Wojzynski MK, Davison DB, Worley KC (1996) BCM search launcher an integrated interface to molecular biology data base search and analysis services available on the World Wide Web. Genome Res 6:454–462
Teifel-Greding J, Baur C, Josephi E, Liebhardt E (1988) Speziesidentifizierung an der Spur mit molekularbiologischen Methoden. Beitr Gerichtl Med 47:545–550
Woese CR, Magrum LJ, Gupta R et al (1980) Secondary structure model for bacterial 16S ribosomal RNA phylogenetic, enzymatic and chemical evidence. Nucleic Acids Res 8:2275–2293
Zehner R, Zimmermann S, Mebs D (1998) RFLP and sequence analysis of the cytochrome b gene of selected animals and man: methodology and forensic application. Int J Leg Med 111:323–327
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Balitzki-Korte, B., Anslinger, K., Bartsch, C. et al. Species identification by means of pyrosequencing the mitochondrial 12S rRNA gene. Int J Legal Med 119, 291–294 (2005). https://doi.org/10.1007/s00414-005-0537-9
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DOI: https://doi.org/10.1007/s00414-005-0537-9