Abstract
Maceration techniques remove soft tissue by the destruction of biomolecules, but the applied techniques may also affect the morphology and the molecular integrity of the hard tissue itself. The impact of seven different techniques for soft tissue removal on morphological and biomolecular parameters of teeth and dental tissues was systematically examined. All methods tested showed significant changes in dental morphology and in the molecular integrity of DNA and the dental proteins, as revealed by aspartic acid racemisation (AAR). In forensic casework this may have severe impacts on the results of morphological methods (e.g. age estimation based on root translucency) and of biomolecular analyses (e.g. age estimation based on AAR and DNA analysis). Therefore, age estimation based on AAR should not be applied to tissue treated in such a manner, and it is recommended that teeth for analysis should be extracted before soft tissue removal. DNA in the hard tissue seems to be less susceptible to soft tissue removal than proteins, and several of the tested maceration techniques appear not to have a damaging effect on DNA. Generally, the indication for soft tissue removal demands a careful case management to avoid methodological collisions.
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Alonso A, Martin P, Albarran C, Garcia P, Garcia O, de Simon LF, Garcia-Hirschfeld J et al (2004) Real-time PCR designs to estimate nuclear and mitochondrial DNA copy number in forensic and ancient DNA studies. Forensic Sci Int 28:141–149
Bartels T, Flachsbarth M, Meyer W (1992) Zu den speziellen Möglichkeiten von Biozym SE in der Mazerations-und Korrosionstechnik. Der Präparator 38:89–96
Brennan TV, Clarke S (1993) Spontaneous degradation of polypeptides at aspartyl and asparaginyl residues—effects of the solvent dielectric. Protein Sci 2:331–338
Bürgi J (1967) Mazeration von unfixierten und fixierten Knochen. Der Präparator 13:185–190
Capasso S, Mazzarella L, Zagari A (1991) Deamidation via cyclic imide of asparaginyl peptides: dependence on salts, buffers and organic solvents. Pept Res 4:234–238
Clarke S (1987) Propensity for spontaneous succinimide formation from aspartyl and asparaginyl residues in cellular proteins. Int J Pept Protein Res 30:808–821
Drusini A (1991) Age-related changes in root transparency of teeth in males and females. Am J Hum Biol 3:629–637
van Duin ACT, Collins MJ (1998) The effects of conformational constraints on aspartic acid racemization. Org Geochem 29:1227–1232
Fu S-J, Fan C-C, Song H-W, Wei F-Q (1995) Age estimation using a modified HPLC determination of ratio of aspartic acid in dentin. Forensic Sci Int 73:35–40
Fujii N, Momose Y, Ishii N, Takita M, Akaboshi M, Kodama M (1999) The mechanism of simultaneous stereoinversion, racemization, and isomerization at specific aspartyl residues of aged lens protein. Mech Ageing Dev 107:347–358
Glauser PW, Kraus-Ruppert R (1979) Über die Herstellung von Mazerationspräparaten eines Oberschenkelkopfes. Der Präparator 25:153–156
Grundmann C, Rötzscher K (1999) Zur Sektionstechnik im orofazialen Bereich. Die Mazeration mit Enzyrim. Rechtsmedizin 9:115–117
Gustafson G (1950) Age determinations on teeth. J Am Dent Assoc 41:45–54
Hoffmeister DF, Lee MR (1963) Cleaning mammalian skulls with ammonium hydroxide. J Mammal 44:283–284
Konrad G, Sparing S (1962) Die Mazeration von Skeletten mittels Natronbleichlauge (Liquor Natrii Hypochlorosi). Der Präparator 8:91–93
Lopez-Nicolas M, Morales A, Luna A (1996) Application of dimorphism in teeth to age calculation. J Forensic Odontostomatol 1:10–13
Lura R, Schirch V (1988) Role of peptide conformation in the rate and mechanism of deamidation of asparaginyl residues. Biochemistry 27:7671–7677
Maples WR (1978) An improved technique using dental histology for estimation of adult age. J Forensic Sci 134:764–770
Maus H-E (1974) Die Mazeration von Knochen mit enzymhaltigen Waschmitteln. Med Diss, Technische Hochschule Aachen
Meinig H, Verwiebe S (1993) Zeitsparende und kostengünstige Methode zur Mazeration von fixierten Kleinsäugermaterial. Der Präparator 39:19–21
Metzger Z, Buchner A, Gorsky M (1980) Gustafson’s method for age determination from teeth—a modification for the use of dentists in identification teams. J Forensic Sci 25:742–749
Mörnstad H, Pfeiffer H, Teivens A (1994) Estimation of dental age using HPLC-technique to determine the degree of aspartic acid racemization. J Forensic Sci 39:1425–1431
Monzavi BF, Ghodoosi A, Savabi O, Hasanzadeh A (2003) Model of age estimation based on dental factors of unknown cadavers among Iranians. J Forensic Sci 48:379–381
Niederklopfer P, Troxler M (2001) Knochenpräparation Handbuch für Praktiker. ROMEI AG Edelstahlverarbeitung, Rothenbrunnen
Ochs K (2002) Produktdeklaration Wasch-und Waschhilfsmittel. Henkel Waschmittel, Düsseldorf
Ohtani S (1995a) Estimation of age from the teeth of unidentified corpses using the amino acid racemization method with reference to actual cases. Am J Forensic Med Pathol 16:238–242
Ohtani S (1995b) Estimation of age from dentin by utilizing the racemization of aspartic acid: influence of pH. Forensic Sci Int 75:181–187
Ohtani S (1995c) Estimation of age from dentin by using the racemization reaction of aspartic acid. Am J Forensic Med Pathol 16:158–161
Ohtani S, Ito R, Arany S, Yamamoto T (2005) Racemization in enamel among different types of teeth from the same individual. Int J Legal Med 119:66–69
Olze A, Knell B, Hauri-Bionda R, Schmeling A, Geserick G (2002) Überprüfung der Altersdiagnostik mittels Wurzeldentintransparenzmessung bei unbekannten Toten. Rechtsmedizin 12:143–149
Piechoki R, Altner H-J (1998) Makroskopische Präparationstechnik. Leitfaden für das Sammeln, Präparieren und Konservieren. Fischer, Jena
Porkert J, Novak J, Grosseova M (1983) Zur Mazeration kleiner Wirbeltiere in alkalischen Lösungen der Halogene. Der Präparator 29:53–54
Prince D, Ubelaker DH (2002) Application of Lamendin’s adult dental aging technique to a diverse skeletal sample. J Forensic Sci 47:940–943
Rennick SL, Fenton TW, Foran DR (2005) The effects of skeletal preparation techniques on DNA from human and non-human bone. J Forensic Sci 50:1016–1019
Ritz S, Schütz HW, Peper C (1993) Postmortem estimation of age at death based on aspartic acid racemization in dentin: its applicability for root dentin. Int J Legal Med 105:289–293
Ritz S, Stock R, Schütz HW, Kaatsch H-J (1995) Age estimation in biopsy specimens of dentin. Int J Legal Med 108:135–139
Ritz-Timme S (1999) Lebensaltersbestimmung aufgrund des Razemisierungsgrades von Asparaginsäure. Grundlagen, Methodik, Möglichkeiten, Grenzen, Anwendungsbereiche. In: Berg S, Brinkmann B (eds) Arbeitsmethoden der medizinischen und naturwissenschaftlichen Kriminalistik, Band 23. Schmidt-Römhild, Lübeck, pp 1–84
Ritz-Timme S, Rochholz G, Schütz HW, Collins MJ, Waite E, Cattaneo C, Kaatsch H-J (2000) Quality assurance in age estimation based on aspartic acid racemization. Int J Legal Med 114:83–86
Ritz-Timme S, Cattaneo C, Collins MJ, Waite ER, Schütz HW, Kaatsch H-J, Borrman HIM (2000) Age estimation: the state of the art in relation to the specific demands of forensic practise. Int J Legal Med 113:129–136
Robinson NE, Robinson AB (2001a) Molecular clocks. Proc Natl Acad Sci USA 98:944–949
Robinson NE, Robinson AB (2001b) Prediction of protein deamidation rates from primary and three-dimensional structure. Proc Natl Acad Sci USA 98:4367–4372
Rösing FW, Kvaal SI (1998) Dental age in adults—a review of estimation methods. In: Alt KW, Rösing FW, Teschler-Nicola M (eds) Dental anthropology. Fundamentals, limits, and prospects. Springer, Berlin Heidelberg New York, pp 443–469
Schmidt P (1974) Haltung von Speckkäfern zur Herstellung von Skeletten kleiner Wirbeltiere. Neue Museumskd 17:216–218
Schmidt U (1981) Mazeration mit Waschmitteln. Der Präparator 27:69–76
Solheim T (1989) Dental root translucency as an indicator of age. Scand J Dent Res 97:189–197
Steinmann WF (1982) Makroskopische Präparationsmethoden in der Medizin. Thieme, Stuttgart
von Wurmb-Schwark N, Schwark T, Harbeck M, Oehmichen M (2004) Amplification of mtDNA and nuclear DNA in a duplex PCR to evaluate the DNA quality of anthropological and forensic samples prior to STR typing. Legal Med 6:80–88
von Wurmb-Schwark N, Ringleb A, Gebühr M, Simeoni E (2005) Genetic analysis of modern and historical burned human remains. Anthropol Anz 63:1–12
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Offele, D., Harbeck, M., Dobberstein, R.C. et al. Soft tissue removal by maceration and feeding of Dermestes sp.: impact on morphological and biomolecular analyses of dental tissues in forensic medicine. Int J Legal Med 121, 341–348 (2007). https://doi.org/10.1007/s00414-006-0116-8
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DOI: https://doi.org/10.1007/s00414-006-0116-8