Abstract
In this study we analyzed the eight Y-STR loci, DYS443, DYS444, DYS448, DYS453, DYS455, DYS456, DYS457 (DYS437) and DYS458, investigated haplotype distributions of these Y-STR loci in a Chinese Han population, and sequenced alleles of the eight loci for clarifying the structure. Extracted DNA was amplified by PCR and the PCR products were analyzed by non-denaturing horizontal polyacrylamide gel electrophoresis with a discontinuous buffer system. Alleles were sequenced on an ABI 3700 using a Dye Terminator Cycle sequencing kit. DYS443, DYS453, DYS455 and DYS456 were found to be simple repeat systems, while DYS444, DYS448, DYS457 (DYS437) and DYS458 were complex repeat systems. The gene diversities of DYS443, DYS444, DYS448, DYS453, DYS455, DYS456, DYS457 (DYS437) and DYS458 were 0.7742, 0.7671, 0.7453, 0.3545, 0.0549, 0.6988, 0.6148 and 0.8213, respectively. The haplotype diversity for 8 Y-STR loci was 0.9996, and the discrimination capacity was 0.9815. The results indicate that these eight loci are useful Y-linked markers for forensic applications.
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References
Allen CR, Graves G, Budowle B (1989) Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver. Biotechniques 7:736–744
Ayub Q, Mohyuddin A, Qamar R, Mazahar K, Zerjal T, Mehdi SQ, Tyler-Smith C (2000) Identification of novel human Y chromosomal microsatellites from sequence database information. Nucleic Acids Res 28:e8
Bär W, Brinkmann B, Lincoln P, Mayr W, Rossi U (1994) DNA recommendations—1994 report concerning further recommendations of the DNA Commission of the ISFH regarding PCR-based polymorphisms in STR (short tandem repeats) systems. Int J Legal Med 107:159–166
Bosch E, Lee A, Calafell F, Arroyo E, Henneman P, Knijff P de, Jobling MA (2002) High resolution Y-chromosome typing: 19 STRs amplified in three multiplex reactions. Forensic Sci Int 125:42–51
Butler JM, Schoske R, Vallone PM, Kline MC, Redd AJ, Hammer MF (2002) A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers. Forensic Sci Int 129:10–24
Caglia A, Dobosz M, Boschi I, d'Aloja E, Pascali VL (1998) Increased forensic efficiency of a STR-based Y-specific haplotype by addition of the highly polymorphic DYS385 locus. Int J Legal Med 111:142–146
Gill P, Brenner C, Brinkmann B et al. (2001) DNA Commission of the International Society of Forensic Genetics: recommendations on forensic analysis using Y-chromosome STRs. Int J Legal Med 114:305–309
Hou Y, Gill P, Schmitt C, Staak M, Prinz M (1994) Population genetics of three STR polymorphisms in a Chinese population. In: Bär W, Fiori A, Rossi U (eds) Advances in forensic haemogenetics 5. Springer, Berlin Heideberg New York, pp 508–510
Hou YP, Zhang J, Li YB, Wu J, Zhang SZ, Prinz M (2001a) Allele sequences of six new Y-STR loci and haplotypes in the Chinese Han population. Forensic Sci Int 118:147–152
Hou Y, Zhang J, Sun D, Li Y, Wu J, Zhang S, Prinz M (2001b) Typing Y chromosome STR haplotypes using redesigned primers. J Forensic Sci 47:215–217
Iida R, Tsubota E, Matsuki T (2001) Identification and characterization of two novel human polymorphic STRs on the Y chromosome. Int J Legal Med 115:54–56
Iida R, Tsubota E, Sawazakik K, Masuyama M, Matsuki T, Yasuda T, Kishi K (2002) Characterization and haplotype analysis of the polymorphic Y-STRs DYS443, DYS444 and DYS445 in a Japanese population. Int J Legal Med 116:191–192
Jobling MA, Tyler-Smith C (1995) Fathers and sons: the Y chromosome and human evolution. Trends Genet 11:449–456
Jobling MA, Pandya A, Tyler-Smith C (1997) The Y chromosome in forensic analysis and paternity testing. Int J Legal Med 110:118–124
Redd AJ, Agellon AB, Kearney VA et al. (2002) Forensic value of 14 novel STR on the human Y chromosome. Forensic Sci Int 130:97–111
Shimada I, Rand S, Brinkmann B, Hohoff C (2002) Kurdish population data for 11 STR loci (ACTBP2, CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D7S820, D13S317 and D21S11). Int J Legal Med 116:301–303
Shin DJ, Jin HJ, Kwak KD, Choi JW, Han MS, Kang PW, Choi SK, Kin W (2001) Y-chromosome multiplex and their potential for the DNA profiling of Koreans. Int J Legal Med 115:109–117
Singer-Sam J, Tanguay RL, Riggs AD (1989) Use of chelex to improve the PCR signal from a small number of cells. Amplification 3:11
Tsai L-C, Yuen T-Y, Hsieh H-M, Lin M, Tzeng C-H, Huang N-W, Linacre A, Lee J G-I (2002) Haplotype frequencies of nine Y-chromosome STR loci in the Taiwanese Han population. Int J Legal Med 116:179–183
Waye JS, Presley LA, Budowle B, Shutler GG, Fourney RM (1989) A simple and sensitive method for quantifying human genomic DNA in forensic specimen extracts. Biotechniques 7:852–855
White PS, Tatum OL, Deaven LL, Longmire L (1999) New, male-specific microsatellite markers from the human Y chromosome. Genomics 57:433–437
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This study was supported by grants from the National Natural Foundation of China (No. 30271446), from the Chinese Medical Board of New York, and from the Foundation of the Ministry of Education of the P.R. China
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Tang, J.P., Hou, Y.P., Li, Y.B. et al. Characterization of eight Y-STR loci and haplotypes in a Chinese Han population. Int J Legal Med 117, 263–270 (2003). https://doi.org/10.1007/s00414-003-0371-x
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DOI: https://doi.org/10.1007/s00414-003-0371-x