Radiation and Environmental Biophysics

, Volume 57, Issue 2, pp 115–121 | Cite as

The level of DNA damage in mouse hematopoietic cells and in frog and human blood cells, as induced by the action of reactive oxygen species in vitro

  • Nikolay Sirota
  • Elena Kuznetsova
  • Irina Mitroshina
Original Article
  • 54 Downloads

Abstract

Comparative studies of the level of DNA damage induced in vitro by X-rays (0–8 Gy) or hydrogen peroxide (0–300 µM) in cells of blood, spleen, and bone marrow of mice and in blood cells of frogs and humans were performed using the alkaline comet assay. For both agents, the levels of induced DNA damage in leucocytes/splenocytes of mice were higher than those in blood cells of frogs and humans, while in human leucocytes, they were comparable with those in frog blood cells. The rate of DNA repair in frog blood cells was very slow. The results suggest that the levels of radiation-induced DNA damage are not in accordance with species radiosensitivity (according to LD50/30) but rather with the intrinsic peculiarities of cells.

Keywords

Comet assay DNA damage X-ray Hydrogen peroxide 

Notes

Acknowledgements

We are grateful to V.K. Uteshev (Institute of Cell Biophysics RAS, Pushchino of Moscow Region) for granting blood samples of Rana temporaria.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human and animal participants

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Nikolay Sirota
    • 1
  • Elena Kuznetsova
    • 1
  • Irina Mitroshina
    • 1
  1. 1.Institute of Theoretical and Experimental Biophysics of the Russian Academy of SciencesPushchinoRussia

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