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Proton-induced direct and indirect damage of plasmid DNA

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Abstract

Clustered DNA damage induced by 10, 20 and 30 MeV protons in pBR322 plasmid DNA was investigated. Besides determination of strand breaks, additional lesions were detected using base excision repair enzymes. The plasmid was irradiated in dry form, where indirect radiation effects were almost fully suppressed, and in water solution containing only minimal residual radical scavenger. Simultaneous irradiation of the plasmid DNA in the dry form and in the solution demonstrated the contribution of the indirect effect as prevalent. The damage composition slightly differed when comparing the results for liquid and dry samples. The obtained data were also subjected to analysis concerning different methodological approaches, particularly the influence of irradiation geometry, models used for calculation of strand break yields and interpretation of the strand breaks detected with the enzymes. It was shown that these parameters strongly affect the results.

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Acknowledgments

This work has been supported by ESA Project No. ESTEC/ITT AO/1-7146/12/NL/GLC “Radiation Biological End Effects Models and Interfaces to Physics Models” and MEYS CR Project No. LD12008. L. Vyšín and L. Juha appreciate a partial financial support provided by the Czech Science Foundation under the Grant No. 13-28721S. M. Vlk is grateful for a partial financial support by MEYS CR Project No. LK21310. The irradiation has been realized at the CANAM (Center of Accelerators and Nuclear Analytical Methods) supported by the MEYS CR Project No. LM 2011019. The authors also appreciate valuable comments of Dr. Amitava Adhikary.

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Authors and Affiliations

  1. Institute of Physics CAS, Na Slovance 1999/2, 182 21, Prague, Czech Republic

    Luděk Vyšín & Libor Juha

  2. FNSPE, Czech Technical University in Prague, Břehová 7, 115 19, Prague, Czech Republic

    Luděk Vyšín & Martin Vlk

  3. Nuclear Physics Institute CAS, Na Truhlářce 39/64, 180 00, Prague, Czech Republic

    Kateřina Pachnerová Brabcová, Richard Wagner & Marie Davídková

  4. CNRS/IN2P3, Centre d’Etudes Nucléaires de Bordeaux-Gradignan, CENBG, Université de Bordeaux, Chemin du Solarium, BP 120, 33 175, Gradignan, France

    Václav Štěpán & Sebastien Incerti

  5. Laboratoire Collisions Agregats Réactivité, IRSAMC, CNRS, UMR 5589, Université de Toulouse, UPS, 31062, Toulouse, France

    Patrick Moretto-Capelle, Pierre Cafarelli, Romain Casta, Jean Philippe Champeaux & Martine Sence

  6. LBCMCP, CNRS, UMR 5088, Université de Toulouse, UPS, 31062, Toulouse, France

    Beatrix Bugler & Gaelle Legube

  7. Nuclear Physics Institute CAS, Husinec – Řež 130, 250 68, Řež, Czech Republic

    Jan Štursa & Václav Zach

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  1. Luděk Vyšín
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  11. Martin Vlk
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  12. Richard Wagner
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  15. Sebastien Incerti
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  16. Libor Juha
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Correspondence to Kateřina Pachnerová Brabcová.

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Vyšín, L., Pachnerová Brabcová, K., Štěpán, V. et al. Proton-induced direct and indirect damage of plasmid DNA. Radiat Environ Biophys 54, 343–352 (2015). https://doi.org/10.1007/s00411-015-0605-6

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  • DOI: https://doi.org/10.1007/s00411-015-0605-6

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