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Clonogenic survival of human keratinocytes and rodent fibroblasts after irradiation with 25 kV x-rays

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Abstract

Low energy x-rays (Eph ≤50 keV) are widely used in diagnostic radiology and radiotherapy. However, data on their relative biological effectiveness (RBE) are scarce. Of particular importance for risk estimation are the RBE values of x-rays in the range which is commonly used in mammography (10–30 keV). We have determined clonogenic survival after low-energy x-ray irradiation for three cell lines: primary human epidermal keratinocytes (HEKn), mouse fibroblasts (NIH/3T3) and Chinese hamster fibroblasts (V79). Experiments were performed with a 25 kV x-ray tube and compared to 200 kV x-rays as a reference. Compared to the effect of 200 kV x-rays, irradiation with 25 kV x-rays resulted in a decreased survival rate in the murine fibroblasts, but not in the human epithelial cell line. The RBE value was calculated for 10% surviving fraction. For HEKn cells, RBE was 1.33±0.27, for NIH/3T3 cells 1.25±0.07 and for V79 cells 1.10±0.09, respectively. No consistently increased RBE was observed in the various cell lines. Nevertheless, a potential of increased cytogenetic changes has to be considered for risk estimation of low-energy x-rays.

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Acknowledgements

This study was supported in part by Sächsisches Staatsministerium für Wissenschaft und Kunst, grants number 4–7531.50–04–0370–99/14 and –00/.1. The study was associated with a project supported by the Deutsche Forschungsgemeinschaft, grant number Do429/4–1.

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Authors and Affiliations

  1. Institute of Nuclear and Hadron Physics, Forschungszentrum Rossendorf, P.O. Box 510119, 01314 , Dresden, Germany

    Anna Panteleeva & Jörg Pawelke

  2. Laboratory of Radiation Biology, Centre of Oncology, Kraków, Poland

    Dorota Słonina

  3. Medical Faculty Carl Gustav Carus, Technical University Dresden, Germany

    Dorota Słonina, Katarina Brankovic, Kathrin Spekl, Cordelia Hoinkis & Wolfgang Dörr

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  1. Anna Panteleeva
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  2. Dorota Słonina
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  3. Katarina Brankovic
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  4. Kathrin Spekl
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  5. Jörg Pawelke
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  6. Cordelia Hoinkis
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  7. Wolfgang Dörr
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Correspondence to Anna Panteleeva.

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Panteleeva, A., Słonina, D., Brankovic, K. et al. Clonogenic survival of human keratinocytes and rodent fibroblasts after irradiation with 25 kV x-rays. Radiat Environ Biophys 42, 95–100 (2003). https://doi.org/10.1007/s00411-003-0199-2

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  • DOI: https://doi.org/10.1007/s00411-003-0199-2

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