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Analyzing the Effect of Vitronectin on Cell Growth and Mesenchymal-Epithelial Transition of Pulmonary Fibroblast Cells

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Abstract

Purpose

Vitronectin (VTN), a multifunctional glycoprotein, is involved in various biological and pathological processes. The purpose of this study was to explore the effect of VTN on mesenchymal-epithelial transition (MET) of pulmonary fibroblast cells.

Methods

Lentivirus encoding for VTN-specific shRNA was constructed and infected into the cultured fibroblast WI-38 cells. Real-time PCR and Western blot were applied to examine the expression of VTN in WI-38 cells. MTT assay was used to assess cell proliferation. Western blot was conducted to examine the expression of MET-related and apoptosis-related proteins.

Results

The knockdown of VTN significantly inhibited the growth of WI-38 cells compared to the control group. Meanwhile, knockdown of VTN remarkably increased the expression of Bax and Caspase 3 compared with the control group. Furthermore, knockdown of VTN significantly promoted the expression of E-cadherin in comparison to control group.

Conclusions

Knockdown of VTN promoted the expression of apoptosis-related factors, meanwhile, facilitated the MET process of fibroblast cells by regulating the expression of relevant factors. In sum, VTN performed a potential regulator in cell growth and MET of pulmonary fibroblast cells, which can be considered as a potential target for diagnose and therapy of relevant diseases.

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Data Availability

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the National Natural Science Foundation of China (No.81572950), Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support (No.20161433), and Shanghai Shen Kang Hospital Development Center Clinical Science and Technology Innovation Project (No.SHDC12017103).

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Authors and Affiliations

  1. Department of Radiation Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 241 West Huaihai Road, Shanghai, China

    Ya Zeng, Jiahua Yu, Mina Liu, Qin Zhang & Xuwei Cai

Authors
  1. Ya Zeng
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  2. Jiahua Yu
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  3. Mina Liu
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  4. Qin Zhang
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  5. Xuwei Cai
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Contributions

XC proposed the concept and the design of experimental scheme of this study. YZ completed the related experiments in this study. JY collated and analyzed the experimental data. ML and QZ cooperated to complete the manuscript. XC reviewed the experimental data and revised the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Xuwei Cai.

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The authors declare that they have no conflict of interest.

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Zeng, Y., Yu, J., Liu, M. et al. Analyzing the Effect of Vitronectin on Cell Growth and Mesenchymal-Epithelial Transition of Pulmonary Fibroblast Cells. Lung 199, 389–394 (2021). https://doi.org/10.1007/s00408-021-00467-1

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  • DOI: https://doi.org/10.1007/s00408-021-00467-1

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