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Effect of Lung Fibrosis on Glycogen Content in Different Extrapulmonary Tissues

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An Erratum to this article was published on 28 February 2014

Abstract

Purpose

Patients with pulmonary fibrosis often exhibit reduced lung function and diminished health-related quality of life. Studies have shown that paraquat-induced, extrapulmonary, acute lung injury affects the metabolic profile of glycogen content in different tissues. The purpose of the present study was to investigate whether the process of pulmonary fibrosis induced by continuous exposure to the toxic herbicide paraquat or by a local insult from bleomycin affects the glycogen content in tissues.

Methods

In the paraquat experiment, Wistar rats (n = 5 per group) received either saline (controls) or an intraperitoneal injection of a paraquat solution (7.0 mg/kg; experimental group) once a week for 4 weeks. In the bleomycin experiment, Balb/c mice (n = 5 per group) received either saline (controls) or 6.25 U/kg of bleomycin through intratracheal instillation in single dose (experimental group). Glycogen content in different tissues (mg/g tissue) was measured using the anthrone reagent. The lungs submitted to histopathological and quantitative analyses of fibrosis.

Results

Paraquat-induced fibrosis led to lower glycogen content in the gastrocnemius muscle (2.7 ± 0.1 vs. 3.4 ± 0.1; 79 %) compared with the controls, whereas no changes in glycogen content were found in the diaphragm or heart. Bleomycin-induced fibrosis led to lower glycogen content in the diaphragm (0.43 ± 0.02 vs. 0.79 ± 0.09, 54 %), gastrocnemius muscle (0.62 ± 0.11 vs. 1.18 ± 0.06, 52 %), and heart (0.68 ± 0.11 vs. 1.39 ± 0.1, 49 %) compared with the controls (p < 0.05). Moreover, the area of fibrous connective tissue (μm2) in the lungs was significantly increased in paraquat-induced fibrosis (3,463 ± 377 vs. 565 ± 89) and bleomycin-induced fibrosis (3,707 ± 433.9 vs. 179 ± 51.28) compared with the controls.

Conclusions

The findings suggest that the effects of fibrogenesis in the lungs are not limited to local alterations but also lead to a reduction in glycogen content in the heart and other muscles. This reduction could partially explain the impaired muscle performance found in patients with pulmonary fibrosis.

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Conflict of interest

The authors declare no competing interests.

Ethical standards

The authors declare that the experiments comply with the current laws of the country in which they were performed. All experiments were performed in compliance with the Ethical Principles in Animal Experimentation adopted by the local Ethics Committee on Animal Experimentation (CETEA/UFMG process No. 60/2006).

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Authors and Affiliations

  1. Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG, 31270-010, Brazil

    Elizabeth Lage Borges, Marina de Barros Pinheiro, Wesley Araújo Sales & Yuri Augusto Junqueira Belém Silva

  2. Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Luana Oliveira Prata & Marcelo Vidigal Caliari

  3. Faculdade de Ciências Médicas de Minas Gerais, Belo Horizonte, MG, Brazil

    Maria da Glória Rodrigues-Machado

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  1. Elizabeth Lage Borges
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  2. Marina de Barros Pinheiro
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  3. Luana Oliveira Prata
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Correspondence to Elizabeth Lage Borges.

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Borges, E.L., de Barros Pinheiro, M., Prata, L.O. et al. Effect of Lung Fibrosis on Glycogen Content in Different Extrapulmonary Tissues. Lung 192, 125–131 (2014). https://doi.org/10.1007/s00408-013-9539-4

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