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High-fat diet-induced biogenesis of pulmonary exosomes in an experimental rat model

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Abstract

Background

High-fat diets (HFD) have recently become a public health concern. We hypothesize that HFD induces exosomes biogenesis in the lung tissue of rat model.

Methods and results

Sixteen adult male Wistar rats were fed with HFD or a regular chow diet for 3 months. The histopathological changes in lung tissues were measured by hematoxylin and eosin (H&E) staining. Bronchoalveolar lavage (BAL) was performed to assay exosomes by acetylcholinesterase enzyme (AhCE) activity. Real-time PCR (qPCR) was used to evaluate Rab27-b, Alix, and IL-1β expression, while the immunohistochemical examination was performed for CD81 expression in lung tissues. In addition, expression of IL-1β was detected by ELISA. We found pathological alterations in the lung tissue of HFD animals. AhCE activity along with the expression level of Rab27-b, Alix, and IL-1β was increased in HFD animals (p < 0.05). Immunohistochemical staining showed that expression of CD81 was increased in lung tissues of HFD animals compared with the control group (p < 0.05).

Conclusion

Hence, HFD induced exosomes biogenesis and histopathological changes with IL-1β expression in rats’ lung tissues.

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Data availability

The data that support the findings of this study are available on request from the corresponding author.

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Acknowledgements

We would like to thank the Clinical Research Development Unit of Zahra Mardani Azari children educational and treatment center, Tabriz university of medical sciences, Tabriz, Iran for their assistance in this research.

Funding

This work was approved and supported by a grant from Tabriz University of Medical Sciences (No: IR.TBZMED.VCR.REC.1399.236).

Author information

Author notes

  1. Mahdi Ahmadi and Jafar Rezaie contributed equally to this work.

Authors and Affiliations

  1. Pediatric Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Maryam Shoaran

  2. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Javad Behmand

  3. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Reza Rahbarghazi & Mahdi Ahmadi

  4. Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Reza Mosaddeghi-Heris

  5. Solid Tumor Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran

    Jafar Rezaie

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  1. Maryam Shoaran
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Contributions

M-Sh and JR conceived and designed the experiments and analyzed the data. RR prepared the figures. JR and MA wrote the manuscript. R M-H, MA, and MJ-B performed the experiments. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Mahdi Ahmadi or Jafar Rezaie.

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Competing interests

The authors have no competing interests to declare.

Ethical approval

The animal experimental procedures were conducted according to the principles of guidelines for the ethical use of animals in applied studies and approved by the Ethics Committee on Animal Use of Tabriz University of Medical Sciences (IR.TBZMED.VCR.REC.1399.236) in compliance with the ARRIVE guidelines.

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Shoaran, M., Behmand, M.J., Rahbarghazi, R. et al. High-fat diet-induced biogenesis of pulmonary exosomes in an experimental rat model. Mol Biol Rep 50, 7589–7595 (2023). https://doi.org/10.1007/s11033-023-08703-y

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  • DOI: https://doi.org/10.1007/s11033-023-08703-y

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