Reduction of dopamine D2/3 receptor binding in the striatum after a single administration of esketamine, but not R-ketamine: a PET study in conscious monkeys
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R-ketamine appears to be a potent, long-lasting and safer antidepressant, relative to esketamine (S-ketamine), since it might be free of psychotomimetic side effects. Using [11C]raclopride and positron emission tomography (PET), we investigated whether esketamine and R-ketamine can affect dopamine D2/3 receptor binding in the conscious monkey brain. A single infusion of esketamine (0.5 mg/kg), but not R-ketamine (0.5 mg/kg), caused a reduction of binding availability of dopamine D2/3 receptor in the monkey striatum. This study suggests that unlike to R-ketamine, esketamine can cause dopamine release in the striatum, and that its release might be associated with psychotomimetic effects of esketamine.
KeywordsDopamine D2/3 receptor Esketamine Release R-ketamine Monkey
The rapid-onset antidepressant effects of the N-methyl-D-aspartate (NMDA) receptor antagonist ketamine have attracted serious attention after it was found that a single sub-anesthetic dose (0.5 mg/kg) of ketamine elicited a rapid antidepressant effect within 1–2 h, in depressed patients, including those with treatment-resistant depression and treatment-resistant bipolar depression [1, 2, 3, 4]. These beneficial effects persist for up to 2 weeks in some patients. A recent meta-analysis demonstrated that ketamine produced a rapid, yet transient, antidepressant effect, with odds ratios for response and transient remission of symptoms at 24 h equaling 9.87 and 14.7, respectively, accompanied by brief psychotomimetic and dissociative effects .
Ketamine (or RS-ketamine) is a racemic mixture containing equal parts of R-ketamine and S-ketamine (esketamine). Esketamine shows an approximately threefold to fourfold greater anesthetic potency and greater undesirable psychotomimetic side effects, compared with R-ketamine . This is related to the fact that esketamine has an approximately fourfold greater affinity for the NMDA receptor relative to R-ketamine . We reported that R-ketamine shows greater potency and longer-lasting antidepressant effects than esketamine in animal depression models, including neonatal dexamethasone exposure, repeated social defeat stress and learned helplessness, and that unlike esketamine, R-ketamine does not induce psychotomimetic side effects and abuse potential in rodents [7, 8, 9]. In addition, we reported that a single dose of esketamine (10 mg/kg), but not R-ketamine (10 mg/kg), resulted in loss of parvalbumin (PV)-positive cells in mouse brain regions, such as the medial prefrontal cortex , suggesting that loss of PV-positive cells may be associated with psychosis.
Dopamine D2/3 receptors have a high-affinity state for endogenous dopamine and low-affinity state. Raclopride is a moderately high-affinity selective antagonist at these states of the dopamine D2/3 receptors. [11C]Raclopride has been used as a positron emission tomography (PET) ligand for characterization dopamine D2/3 receptors in the brain from human and monkey. Interestingly, PET using [11C]raclopride would be useful for detection of release of endogenous dopamine from presynaptic terminal . Given differential effects of ketamine enantiomers in the brain, the present study using PET was performed to examine whether a single infusion of ketamine enantiomers could affect the release of endogenous dopamine in the conscious monkey brain.
Animals and drugs
Experiments were conducted in accordance with the recommendations of the US National Institutes of Health. The following experiments were approved by the Ethical Committee of the Central Research Laboratory, Hamamatsu Photonics (Hamamatsu, Shizuoka, Japan). Four male rhesus monkeys (Macaca mulatta; 7.1 ± 1.3 years old, weighing 7.2 ± 1.1 kg) were studied 3 times (saline, esketamine and R-ketamine). The order of PET scan was saline, esketamine and R-ketamine with 2-week period between each scan. The doses (0.5 mg/kg) of esketamine or R-ketamine hydrochloride were based on a previous monkey study  and human studies [1, 2, 3, 4]. Esketamine hydrochloride and R-ketamine hydrochloride were synthesized by K.H. at Chiba University (Chiba, Japan) and were dissolved in physiological saline.
Preparation of [11C]raclopride and PET experiments
[11C]Raclopride was labeled by N-methylation of respective nor compound with [11C]methyl triflate prepared from [11C]methyl iodide. The radioactive purity of [11C]raclopride was greater than 98 %, and the specific radioactivity was 32.8 ± 5.6 GBq/μmol (mean ± SD, n = 12). A high-resolution animal PET scanner (SHR-7700; Hamamatsu Photonics, Hamamatsu, Japan) with a transaxial resolution of 2.6-mm full width half maximum in the enhanced 2D mode and a center-to-center distance of 3.6 mm  was used. PET images were reconstructed by a filtered back projection method with a 4.5-mm Hanning filter, resulting in an in-plane reconstructed resolution of 4.5 mm. PET scans with [11C]raclopride were performed without arterial blood sampling. The trained animal’s head was rigidly fixed to the upper frame of a monkey chair using an acrylic head-restraining device. The animal sitting in a restraining chair was placed at a fixed position in the PET gantry with stereotactic coordinates aligned parallel to the orbitomeatal line. Transmission data with a 68Ge–68Ga pin source were obtained for an attenuation correction.
The data are shown as the mean ± standard error of the mean (S.E.M.). Analysis was performed using PASW Statistics 20 (formerly SPSS Statistics; SPSS). Comparisons between groups were made using the repeated-measures analysis of variance (ANOVA), followed by post hoc Bonferroni test. The P values of less than 0.05 were considered statistically significant.
Marked accumulation of radioactivity in the striatum was shown after an intravenous administration of [11C]raclopride (Fig. 1). Radioactivity in the striatum of esketamine-treated monkeys was lower than those of vehicle (saline)-treated monkeys (Fig. 1). Repeated-measures ANOVA demonstrated a significant change among three conditions in the striatum (F = 70.252, P < 0.001). Post hoc Bonferroni test revealed that a single infusion of esketamine significantly (P = 0.004) decreased BPND in the striatum of monkey brain (Fig. 1). In contrast, a single infusion of R-ketamine did not decrease BPND in the striatum of monkey brain (P = 0.603) (Fig. 1).
Using a conscious PET study, we found that a single infusion of esketamine, but not R-ketamine, elicited a significant reduction of BPND of dopamine D2/3 receptor in the striatum of monkeys. Since [11C]raclopride-PET has been used for detection of release of endogenous dopamine from presynaptic terminal , this study suggests that esketamine, but not R-ketamine, causes the marked release of endogenous dopamine from presynaptic terminal in the striatum of monkeys.
Clinical use of ketamine is limited due to its side effects such as psychotomimetic effects . Unlike esketamine, R-ketamine might not appear to cause psychotomimetic effects, based on the lack of behavioral abnormalities (e.g., hyperlocomotion, prepulse inhibition deficits) observed in rodents after treatment . Recently, Singh et al.  reported a rapid-onset antidepressant effect of esketamine in treatment-resistant patients with depression although Brief Psychiatric Rating Scale (BPRS) score and Clinician Administered Dissociative States Scale (CADSS) score were the highest at 40 min after an infusion of esketamine (0.20 or 0.40 mg/kg for 40 min). Furthermore, an infusion of esketamine, but not R-ketamine, in healthy subjects produced a dissociative state and psychotic syndrome, including disturbances of emotion and sensory perception, difficulties in thinking and reality appraisal, as well as ego disorders . Subsequently, Vollenweider et al.  reported that an infusion of esketamine caused the reduction of in vivo binding of [11C]raclopride binding to dopamine D2/3 receptors in human brain, indicating an increase of dopamine in the striatum. Taken together, it is likely that esketamine-associated psychotomimetic effects in patients might be associated with marked dopamine release in the striatum. Given the role of dopamine release in the striatum for ketamine (or esketamine)-induced psychotomimetic effects, it is unlikely that R-ketamine might cause psychotomimetic effects in monkeys or humans.
Despite an increasing number of studies focusing on the rapid antidepressant effects of ketamine in treatment-resistant depression, its potential to elicit abuse liability cannot be ignored . In contrast, a schedule of repeated ketamine infusions could provide effective management of depressive symptoms in patients with treatment-resistant depression . A study using the conditioned place preference (CPP) test showed that ketamine (1.0–10 mg/kg) significantly increased CPP scores in mice, in a dose-dependent manner , suggesting that ketamine has rewarding effects. We also reported that repeated administration of esketamine (5, 10 or 20 mg/kg), but not R-ketamine (5, 10 or 20 mg/kg), significantly increased CPP scores in mice, in a dose-dependent manner . Taken together, it is possible that release of dopamine by esketamine could induce rewarding effects in rodents . Given the role of dopamine release in the striatum for ketamine-induced rewarding effects, it is unlikely that R-ketamine might cause rewarding effects in monkeys or humans. These findings suggest greater safety for repeated intermittent dosing with R-ketamine relative to esketamine.
In conclusion, this study demonstrates that a single infusion of esketamine, but not R-ketamine, could cause a marked reduction of BPND of dopamine D2/3 receptor in the striatum of conscious monkeys. Therefore, relative to esketamine, R-ketamine is likely to be a safer antidepressant in the treatment of depression.
This study was supported by a Grant-in-Aid for Scientific Research on Innovative Areas of the Ministry of Education, Culture, Sports, Science and Technology, Japan (to K.H., #24116006).
Compliance with ethical standards
Conflict of interest
Dr. Hashimoto is an inventor on a filed patent application on “The use of R-ketamine in the treatment of psychiatric diseases” by Chiba University. K.H. has served as a scientific consultant to Astellas, Dainippon-Sumitomo and Taisho, and he has also received research support from Abbvie, Dainippon-Sumitomo, Mochida, Otsuka and Taisho. The remaining authors declare no conflicts of interest.
- 2.Diazgranados N, Ibrahim L, Brutsche NE, Newberg A, Kronstein P, Khalife S, Kammerer WA, Quezado Z, Luckenbaugh DA, Salvadore G, Machado-Vieira R, Manji HK, Zarate CA Jr (2010) A randomized add-on trial of an N-methyl-D-aspartate antagonist in treatment-resistant bipolar depression. Arch Gen Psychiatry 67:793–802CrossRefPubMedPubMedCentralGoogle Scholar
- 4.Zarate CA Jr, Brutsche NE, Ibrahim L, Franco-Chaves J, Diazgranados N, Cravchik A, Selter J, Marquardt CA, Liberty V, Luckenbaugh DA (2012) Replication of ketamine’s antidepressant efficacy in bipolar depression: a randomized controlled add-on trial. Biol Psychiatry 71:939–946CrossRefPubMedPubMedCentralGoogle Scholar
- 5.Newport DJ, Carpenter LL, McDonald WM, Potash JB, Tohen M, Nemeroff CB, APA Council of Research Task Force on Novel Biomarkers and Treatments (2015) Ketamine and other NMDA antagonists: early clinical trials and possible mechanisms in depression. Am J Psychiatry 172:950–956CrossRefPubMedGoogle Scholar
- 10.Tsukada H, Harada N, Nishiyama S, Ohba H, Kakiuchi T (2000) Cholinergic neuronal modulation alters dopamine D-2 receptor availability in vivo by regulating receptor affinity induced by facilitated synaptic dopamine turnover? Positron emission tomography studies with microdialysis in the conscious monkey brain. J Neurosci 20:7067–7073PubMedGoogle Scholar
- 13.Innis RB, Cunningham VJ, Delforge J, Fujita M, Gjedde A, Gunn RN, Holden J, Houle S, Huang SC, Ichise M, Iida H, Ito H, Kimura Y, Koeppe RA, Knudsen GM, Knuuti J, Lammertsma AA, Laruelle M, Logan J, Maguire RP, Mintun MA, Morris ED, Parsey R, Price JC, Slifstein M, Sossi V, Suhara T, Votaw JR, Wong DF, Carson RE (2007) Consensus nomenclature for in vivo imaging of reversibly binding radioligands. J Cereb Blood Flow Metab 27:1533–1539CrossRefPubMedGoogle Scholar
- 15.Singh JB, Fedgchin M, Daly E, Xi L, Melman C, De Bruecker G, Tadic A, Sienaert P, Wiegand F, Manji H, Drevets WC, Van Nueten L (2016) Intravenous esketamine in adult treatment-resistant depression: a double-blind, double-randomization, placebo-controlled study. Biol Psychiatry. doi: 10.1016/j.biopsych.2015.10.018 PubMedGoogle Scholar
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