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The effect of locally administered phenytoin on wound healing in an experimental nasal septal perforation animal model

  • Rhinology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Purpose

In the present study, we aimed to investigate the effect of phenytoin (PHT) on wound healing in an experimental nasal septal perforation (NSP) model created in rats.

Methods

Twenty-two Sprague Dawley male rats were randomized into two groups. NSP, approximately 2 mm in diameter, was created in each subject. The 1% PHT cream (Study group) and saline (Control group) were applied topically once a day for 14 days. The subjects were sacrificed at the end of 14th day and the nasal septum of each animal was excised for histopathological examination. The epithelial regeneration and degeneration of the nasal mucosa, acute inflammatory cells number, fibroblast number, collagen density, vascularization, eosinophil number, granulation tissue formation, giant cell number, degeneration and regeneration of the nasal cartilage were examined. The macroscopic closure rate of NPSs and histopathological parameters were analyzed statistically.

Results

In this study, the epithelial regeneration, the fibroblast count, the collagen density, the vascularization and the amount of granulation tissue and the macroscopic clouse rate of NSPs were significantly higher in the PHT group than in the saline group (p < .05). There was no significant difference was in other comparisons (p > .05).

Conclusion

The locally administered phenytoin may accelerate wound healing of nasal septum in an experimental NSP model. Consequently, phenytoin might be used as an effective substance to prevent the formation of NSP.

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Funding

The authors did not receive support from any organization for the submitted work.

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Authors and Affiliations

  1. Cerrahpasa Medicine Faculty ENT Department, Istanbul University-Cerrrahpasa, Kocamustafapaşa, Fatih, Istanbul, 34300, Turkey

    Doğan Çakan

  2. University of Health Sciences Istanbul Haseki Training and Research Hospital ENT Department, Istanbul, Turkey

    Semih Uşaklıoğlu

Authors
  1. Doğan Çakan
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  2. Semih Uşaklıoğlu
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Contributions

Material preparation, data collection and analysis was performed by DÇ, statistical analysis was performed by SU. The first draft of the manuscript was written by DÇ and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Doğan Çakan.

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Conflict of interest

The authors have no conflicts of interest to declare that are relevant to the content of this article.

Ethics approval

This experimental study was conducted in Bezmialem Foundation University Experimental Animal Application and Research Center with approvel of Bezmialem Foundation University's Ethics Committee for Animal Experiments (decision date/number: 22.02.2021/32).

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Çakan, D., Uşaklıoğlu, S. The effect of locally administered phenytoin on wound healing in an experimental nasal septal perforation animal model. Eur Arch Otorhinolaryngol 279, 3511–3517 (2022). https://doi.org/10.1007/s00405-022-07276-z

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  • DOI: https://doi.org/10.1007/s00405-022-07276-z

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