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ATF5 involved in radioresistance in nasopharyngeal carcinoma by promoting epithelial-to-mesenchymal phenotype transition

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A Correction to this article was published on 19 May 2020

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Abstract

Purpose

This study aimed to investigate the effects of activating transcription factor-5 (ATF5) on nasopharyngeal carcinoma (NPC) cell radioresistance.

Methods

HONE-1 nasopharyngeal carcinoma cells were irradiated by conventional fractionation to generate HONE-1R radiotherapy-resistant cells. Short hairpin RNA (shRNA) expression plasmids targeting the ATF5 gene were constructed and transfected into the HONE-1R cell line. The proliferation assay, colony formation analysis, Transwell Boyden chamber assay and other experimental methods were performed to verify changes in the radiosensitivity and other biological of NPC cells.

Results

X-ray irradiation significantly promoted the upregulation of ATF5 protein levels in HONE-1 cells, and the protein expression of ATF5 increased with the dose of X-ray irradiation (p < 0.05). The colony formation rate, cell survival rate and migration ability of HONE-1R cells were significantly higher than those of HONE-1 cells (p < 0.05). Simultaneously, X-ray could also promote the morphology of epithelial-mesenchymal transition (EMT) in HONE-1 cells, inducing a lower expression level of E-cadherin and a higher expression level of N-cadherin in a dose-dependent manner (p < 0.05). Inhibiting ATF5 significantly reduced the colony formation rate, cell survival rate, migration and invasiveness of HONE-1R cells (p < 0.05). Moreover, sensitizing HONE-1R cells to X-ray irradiation significantly upregulated the expression of E-cadherin and downregulated the expression of N-cadherin in these cells (p < 0.05).

Conclusions

ATF5 may be a potential therapeutic target to improve radiosensitivity in NPC.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Change history

  • 19 May 2020

    The article ATF5 involved in radioresistance in nasopharyngeal carcinoma by promoting epithelial-to-mesenchymal phenotype transition, written by Yu Shuai, Erxi Fan, Qiuyue Zhong, Guangyong Feng, Qiying Chen, Xiaoxia Gou, Guihai Zhang, was originally published.

Abbreviations

NPC:

Nasopharyngeal carcinoma

ATF5:

Activating transcription factor-5

DAB:

Diaminobenzidine

shRNA:

Short hairpin RNA

DAPI:

4′-6-Diamidino-2-phenylindole

FBS:

Foetal bovine serum

IMRT:

Intensity-modulated radiation therapy

EMT:

Epithelial-mesenchymal transition

MRLC:

Myosin regulatory light chain

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Acknowledgements

This work was supported by the National Natural Science Foundation funding Project.

Funding

National Natural Science Foundation funding Project (Number: 81260370).

Author information

Author notes

  1. Yu Shuai, Erxi Fan and Qiuyue Zhong contributed equally to this work.

Authors and Affiliations

  1. Department of Head and Neck Oncology, Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou Province, People’s Republic of China

    Yu Shuai, Erxi Fan, Qiuyue Zhong, Guangyong Feng, Qiying Chen, Xiaoxia Gou & Guihai Zhang

  2. Department of Oncology, Zhuhai Hospital Affiliated with Jinan University, Guangdong Province, Zhuhai, People’s Republic of China

    Guihai Zhang

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  1. Yu Shuai
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  5. Qiying Chen
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Contributions

Guihai developed and planned the study. Yu, Erxi and Qiuyue performed the irradiation and were responsible for management of the cells. Yu, Erxi and Guangyong performed the experiments and Analyzed the data. All authors participated drafted the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Guihai Zhang.

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Conflict of interest

The authors declare that they have no competing interest.

Ethical approval

Ethical approval this study was approved by the Affiliated Hospital of Zunyi Medical University institutional review board (IRB).

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The original version of this article was revised due to the retrospective open access cancellation request.

The original version of this article was revised due to retrospective open access cancellation order.

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Shuai, Y., Fan, E., Zhong, Q. et al. ATF5 involved in radioresistance in nasopharyngeal carcinoma by promoting epithelial-to-mesenchymal phenotype transition. Eur Arch Otorhinolaryngol 277, 2869–2879 (2020). https://doi.org/10.1007/s00405-020-05996-8

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