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Effects of submucosal PRP injection on wound healing in endonasal surgeries: an experimental study

  • Rhinology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Purpose

To investigate the effects of submucosal platelet-rich plasma (PRP) injection on nasal mucosal wound healing after endoscopic sinus surgery.

Methods

24 New Zealand white rabbits were randomly divided into three groups. To mimic surgery, injury was created using 3-mm punch forceps on ventral turbinate mucosa for all groups. Submucosal PRP was injected to the damaged mucosa in the first group (PRP group, n = 8), where 0.9% saline solution to the second group (saline group, n = 8) and no injection to the third one (control group, n = 8). All the animals were sacrificed 14 days after surgery; histopathological examination and hydroxyproline measurements were performed on the mucosa of all groups.

Results

Neutrophils, goblet cells, and collagen intensity were found significantly lower (p < 0.05) in PRP group than the control group. Also, the number of ciliary and goblet cells, and collagen intensity were found significantly lower (p < 0.05) in PRP group, than the saline group. In PRP group, hydroxyproline levels were found (p < 0.05) significantly lower than the saline and the control group, and no significant difference was found between the saline group and the control group (p > 0.05).

Conclusion

There are many studies in literature showing the positive effects of PRP on wound healing. The results of this study also demonstrated positive effects of PRP on the nasal mucosa. According to these results, PRP injection to the injured nasal mucosa showed anti-inflammatory, mucus-softening, and synechia-reducing effects. Therefore, submucosal PRP injection after endonasal surgeries can be considered an effective application for maintaining nasal physiology.

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Funding

This study was supported by the Commission of Scientific Research Projects of Ondokuz Mayis University (Project No: PYO.TIP.1904.17.016).

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Authors and Affiliations

  1. Department of Otolaryngology and Head and Neck Surgery, Ondokuz Mayis University School of Medicine, Tip Fak. KBB Anabilim Dali, Kurupelit, Samsun, 55139, Turkey

    Ugur Yildirim, Ozgur Kemal & Sinan Atmaca

  2. Department of Pharmacology and Toxicology, Ondokuz Mayis University Veterinary Medicine Faculty, Kurupelit, Samsun, 55139, Turkey

    Abdurrahman Aksoy

  3. Department of Pathology, Ondokuz Mayis University Veterinary Medicine Faculty, Kurupelit, Samsun, 55139, Turkey

    Efe Karaca

  4. Department of Public Health, Ondokuz Mayis University School of Medicine, Kurupelit, Samsun, 55139, Turkey

    Ozlem Terzi

Authors
  1. Ugur Yildirim
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  2. Ozgur Kemal
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  3. Abdurrahman Aksoy
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  4. Efe Karaca
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  6. Sinan Atmaca
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Corresponding author

Correspondence to Ugur Yildirim.

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The authors declare that they have no conflict of interest.

Ethical standards

Local Ethics Committee for Animal Studies of Ondokuz Mayis University approved the experimental procedures (31 March 2017-Decree no: 2017/17).

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Yildirim, U., Kemal, O., Aksoy, A. et al. Effects of submucosal PRP injection on wound healing in endonasal surgeries: an experimental study. Eur Arch Otorhinolaryngol 277, 1681–1689 (2020). https://doi.org/10.1007/s00405-020-05884-1

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  • DOI: https://doi.org/10.1007/s00405-020-05884-1

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