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Proteomics analysis of pleomorphic adenoma of the human parotid gland

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Abstract

The objective of this study is to perform proteomic analysis of pleomorphic adenoma (PA) in the human parotid gland (PG) with comparison of normal PG. This is an individual prospective randomized controlled trial. This study was performed in a tertiary referral center. Tissue samples of PG and PA were taken after surgical excision of PG from 13 patients. Protein extracts were prepared and protein pools created from the soluble extracts were subjected to 2D-DIGE analysis. Proteins displaying regulation in their abundance were determined and identified using MALDIT-OF/TOF analysis. The identified proteins were subjected to STRING analysis for classification of the proteins based on their biological roles in metabolic pathways. Fifteen proteins, carbonic anhydrase 1, carbonic anhydrase 2, fibrinogen beta chain, alpha-amylase 1, heats hock protein hsp 90-alpha, clusterin, 78 kDa glucose-regulated protein, endoplasmin, alpha-amylase 2b, ATP synthase subunit alpha (mitochondrial), elongation factor 1-gamma, malate dehydrogenase, cytoplasmic, triosephosphate isomerase, receptor of activated protein c kinase 1, and aconitate hydratase, mitochondrial were down-regulated, whereas 11 proteins including ig kappa chain c region, serotransferrin, vimentin, annexin a5, glial fibrillary acidic protein, calreticulin, cartilage oligomeric matrix protein, microfibril-associated glycoprotein 4, 14-3-3 protein epsilon, fibulin-5, and f-box only protein 2 were up-regulated in PA samples in comparison to healthy parotid tissue. This study described the differences observed in protein expression patterns of the PA and normal PG. The results may provide new insights into the pathogenesis and diagnosis of PA in human PG.

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References

  1. Spiro RH (1986) Salivary neoplasms: overview of a 35-year experience with 2807 patients. Head Neck Surg 8:177–184

    Article  CAS  PubMed  Google Scholar 

  2. Rossi ED, Wong LO, Bizzarro T, Petrone G, Mule A, Fadda G, Baloch ZM (2016) The impact of FNAC in the management of salivary gland lesions: institutional experiences leading to a risk-based classification scheme. Cancer Cytopathol 124(6):388–396

    Article  PubMed  Google Scholar 

  3. Jain R, Gupta R, Kudesia M, Singh S (2013) Fine needle aspiration cytology in diagnosis of salivary gland lesions: a study with histologic comparison. Cyto J 10:5

    Google Scholar 

  4. Donadio E, Giusti L, Seccia V, Ciregia F, da Valle Y, Dallan I et al (2013) A new insight into benign tumors of major salivary glands by proteomic approach. PLoS One 8(8):71874

    Article  Google Scholar 

  5. Akpinar G, Kasap M, Aksoy A, Duruksu G, Gacar G, Karaoz E (2014) Phenotypic and proteomic characteristics of human dental pulp derived mesenchymal stem cells from a natal, an exfoliated deciduous, and an impacted third molar tooth. Stem Cells Int 2014:457059

    Article  PubMed  PubMed Central  Google Scholar 

  6. Szklarczyk D, Franceschini A, Wyder S, Forslund K, Heller D, Huerta-Cepas J et al (2015) STRING v10: protein–protein interaction networks, integrated over the tree of life. Nucleic Acids Res 43:447–452

    Article  Google Scholar 

  7. Mi H, Muruganujan A, Casagrande JT (2013) Thomas PD Large-scale gene function analysis with the PANTHER classification system. Nat Protoc 8(8):1551–1566

    Article  PubMed  Google Scholar 

  8. Chopra DP, Xue-Hu IC (1993) Secretion of alpha-amylase in human parotid gland epithelial cell culture. J Cell Physiol 155(2):223–233

    Article  CAS  PubMed  Google Scholar 

  9. Morley DJ (1988) Hodes ME amylase expression in human parotid neoplasms: evidence by in situ hybridization for lack of transcription of the amylase gene. J Histochem Cytochem 36(5):487–491

    Article  CAS  PubMed  Google Scholar 

  10. Prasad KN, Carvalho E, Edwards-Prasad J, La Rosa FG, Kumar S, Kim JH et al (1994) Establishment of human parotid pleomorphic adenoma cells in culture: morphological and biochemical characterization. Vitro Cell Dev Biol Anim 30(5):312–320

    Article  Google Scholar 

  11. Pucci S, Bonanno E, Pichiorri F, Angeloni C, Spagnoli LG (2004) Modulation of different clusterin isoforms in human colon tumorogenesis. Oncogene 23(13):2298–2304

    Article  CAS  PubMed  Google Scholar 

  12. Leskov KS, Klokov DY, Li J, Kinsella TJ, Boothman DA (2003) Synthesis and functional analysis of nuclear clusterin, a cell death protein. J Biol Chem 278(13):11590–11600

    Article  CAS  PubMed  Google Scholar 

  13. Xie D, Sham JS, Zeng WF, Che LH, Zhang M, Wu HX et al (2005) Oncogenic role of clusterin overexpression in multistage colorectal tumorogenesis and progression. World J Gastroenterol 11(21):3285–3289

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Yu SY, Hong LC, Feng J, Wu YT, Zhang YZ (2016) Integrative proteomics and transcriptomics identify novel invasive-related biomarkers of non-functioning pituitary adenomas. Tumour Biol 37(7):8923–8930

    Article  CAS  PubMed  Google Scholar 

  15. Vanmuylder N, Evrard L, Daelemans P, Dourov N (2000) Chaperones in the parotid gland: localization of heat shock proteins in human adult salivary glands. Cells Tissues Organs 167(2–3):199–205

    Article  CAS  PubMed  Google Scholar 

  16. Wang G, Gu X, Chen L, Wang Y, Cao B, Qun E (2013) Comparison of the expression of 5 heat shock proteins in benign and malignant salivary gland tumor tissues. Oncol Lett 5(4):1363–1369

    PubMed  PubMed Central  Google Scholar 

  17. Zhang Z, Zhang Y, Liu J (2001) Prognostic significance of heat shock proteins 70 and p27 protein in patients with mucoepidermoid carcinoma. Zhonghua Kou Qiang Yi Xue Za Zhi 36(6):467–470

    CAS  PubMed  Google Scholar 

  18. Morrison DK (2009) The 14-3-3 proteins: integrators of diverse signaling cues that impact cell fate and cancer development. Trends Cell Biol 19(1):16–22

    Article  CAS  PubMed  Google Scholar 

  19. Sofiadis A, Becker S, Hellman U, Hultin-Rosenberg L, Dinets A, Hulchiy M et al (2012) Proteomic profiling of follicular and papillary thyroid tumors. Eur J Endocrinol 166(4):657–667

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Leader M, Collins M, Patel J, Henry K (1987) Vimentin: an evaluation of its role as a tumour marker. Histopathology 11(1):63–72

    Article  CAS  PubMed  Google Scholar 

  21. Sedassari BT, Dos Santos HT, Mariano FV, da Silva Lascane NA, Altemani A, Sousa S (2015) Carcinoma ex pleomorphic adenoma of minor salivary glands with major epithelial–myoepithelial component: clinicopathologic and immunohistochemical study of 3 cases. Ann Diagn Pathol 19(3):164–168

    Article  PubMed  Google Scholar 

  22. Burns BF, Dardick I, Parks WR (1988) Intermediate filament expression in normal parotid glands and pleomorphic adenomas. Virchows Arch A Pathol Anat Histopathol 413(2):103–112

    Article  CAS  PubMed  Google Scholar 

  23. Wittchow R, Landas SK (1991) Glial fibrillary acidic protein expression in pleomorphic adenoma, chordoma, and astrocytoma. A comparison of three antibodies. Arch Pathol Lab Med 115(10):1030–1033

    CAS  PubMed  Google Scholar 

  24. Stead RH, Qizilbash AH, Kontozoglou T, Daya AD, Riddell RH (1988) An immunohistochemical study of pleomorphic adenomas of the salivary gland: glial fibrillaryacidic protein-like immunoreactivity identifies a major myoepithelial component. Hum Pathol 19(1):32–40

    Article  CAS  PubMed  Google Scholar 

  25. Takahashi H, Fujita S, Tsuda N, Tezuka F, Okabe H (1991) Iron-binding proteins in adenoid cystic carcinoma of salivary glands: an immunohistochemical study. Tohoku J Exp Med 163(1):1–16

    Article  CAS  PubMed  Google Scholar 

  26. Blankenberg FG (2008) In vivo detection of apoptosis. J Nucl Med 49(2):81–95

    Article  Google Scholar 

  27. Thongwatchara P, Promwikorn W, Srisomsap C, Chokchaichamnankit D, Boonyaphiphat P, Thongsuksai P (2012) Differential protein expression in primary breast cancer and matched axillary node metastasis. Oncol Rep 26(1):185–191

    Google Scholar 

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Acknowledgements

The Scientific Research Unit of Kocaeli University supports this study as a scientific research project (Project Number: 2015/41).

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Authors and Affiliations

  1. Department of Otolaryngology, Kocaeli University Faculty of Medicine, 41380, Kocaeli, Turkey

    Ahmet Mutlu & Murat Ozturk

  2. Uskudar State Hospital, Barbaros Mahallesi, Veysi Pasa Sokak No:14, 34672, Uskudar, Istanbul, Turkey

    Ahmet Mutlu

  3. Department of Medical Biology and Genetics, DEKART Proteomics Laboratory, Kocaeli University Faculty of Medicine, 41380, Kocaeli, Turkey

    Gurler Akpinar, Murat Kasap & Aylin Kanli

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  1. Ahmet Mutlu
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  2. Murat Ozturk
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  3. Gurler Akpinar
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Correspondence to Ahmet Mutlu.

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Mutlu, A., Ozturk, M., Akpinar, G. et al. Proteomics analysis of pleomorphic adenoma of the human parotid gland. Eur Arch Otorhinolaryngol 274, 3183–3195 (2017). https://doi.org/10.1007/s00405-017-4608-6

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  • DOI: https://doi.org/10.1007/s00405-017-4608-6

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