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Expressions of nuclear factor-kappa B p50 and p65 and their significance in the up-regulation of intercellular cell adhesion molecule-1 mRNA in the nasal mucosa of allergic rhinitis patients

  • Rhinology
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Abstract

The purpose of the study was to investigate expressions of nuclear factor-kappa B (NF-κB) and intercellular cell adhesion molecule-1 mRNA (ICAM-1 mRNA) in the nasal mucosa of allergic rhinitis (AR) patients. Expressions of NF-κB and ICAM-1 mRNA were studied using immunohistochemistry and reverse transcription-PCR (RT-PCR) in AR tissues and corresponding normal nasal mucosa. The correlation between NF-κB and ICAM-1 mRNA was studied using linear correlation analysis. The results of immunohistochemistry showed that expression of NF-κB was significantly up-regulated in the nasal mucosa of AR compared with that in normal tissue (P < 0.01), over-expression of NF-κB p50 was found in the cytoplasm and nucleus (P < 0.01), and NF-κB p65 was mainly expressed in the cytoplasm (P < 0.01). ICAM-1 mRNA was strongly expressed in the nasal mucosa of AR compared with that in normal tissue as shown by RT-PCR (P < 0.01). Up-regulation of ICAM-1 mRNA was significantly correlated with over-expressions of NF-κB p50 and NF-κB p65 (r = 0.8995, P < 0.01; r = 0.7601, P < 0.01). In conclusion, NF-κB plays a key role in AR. Excessively activated NF-κB promotes the transcription of ICAM-1 mRNA. ICAM-1 is related to the pathogenesis and development of AR.

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The authors declared that they have no conflicts of interest.

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Authors and Affiliations

  1. Department of Otolaryngology, Head and Neck Surgery, The Fourth People’s Hospital of Ji’nan, 50# Teacher Training Road, Ji’nan, 250031, Shandong, China

    Shi Zhen Wang

  2. Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Shandong University, 247 Beiyuan Street, Ji’nan, 250033, Shandong, China

    Jian Dong Zhao

  3. Reproductive and Genetic Center, Affiliated Hospital of Shandong, University of Traditional Chinese Medicine, Ji’nan, China

    Feng Mei Ma

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  1. Shi Zhen Wang
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  2. Feng Mei Ma
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  3. Jian Dong Zhao
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Correspondence to Jian Dong Zhao.

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Shizhen Wang and Fengmei Ma are contributed equally to this work

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Wang, S.Z., Ma, F.M. & Zhao, J.D. Expressions of nuclear factor-kappa B p50 and p65 and their significance in the up-regulation of intercellular cell adhesion molecule-1 mRNA in the nasal mucosa of allergic rhinitis patients. Eur Arch Otorhinolaryngol 270, 1329–1334 (2013). https://doi.org/10.1007/s00405-012-2136-y

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  • DOI: https://doi.org/10.1007/s00405-012-2136-y

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