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Metabolomic analysis of endometrial cancer by high-resolution magic angle spinning NMR spectroscopy

  • Gynecologic Oncology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

To analyze endometrial metabolite profiles between patients with endometrial cancer and controls.

Methods

Seventeen (17) women with endometrium cancer and 18 controls were enrolled in this study. 1H HR-MAS (High Resolution-Magic Angle Spinning) NMR (Nuclear Magnetic Resonance) spectroscopy data obtained from endometrial tissue samples of patients with endometrial cancer and control group were analyzed with bioinformatics methods.

Results

Principal component analysis (PCA) and the partial least squares discriminant analysis (PLS-DA) score plots obtained with the multivariate statistical analysis of pre-processed spectral data shows a separation between the samples from patients with endometrial cancer and controls. Analysis results suggest that the levels of lactate, glucose, o-phosphoethanolamine, choline, glycerophosphocholine, phosphocholine, leucine, isoleucine, valine, glutamate, glutamine, n-acetyltyrosine, methionine, taurine, alanine, aspartate and phenylalanine are increased in patients with endometrial cancer compared to the controls.

Conclusion

The metabolomics signature of patients with endometrial cancer is different from that of benign endometrial tissue.

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Funding

The study was supported by Scientific Research Projects Unit of Inonu University [under Grant number TCD-2021-2359]. B.D. is supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK) under project number 120C152.

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Authors and Affiliations

  1. Reproductive Sciences & Advanced Bioinformatics Application & Research Center, Inonu University, Malatya, Turkey

    Senem Arda Düz, Akın Mumcu, Berat Doğan, Erdinç Sarıdogan, Görkem Tuncay & Abdullah Karaer

  2. Department of Obstetrics and Gynecology, School of Medicine, Inonu University, 44315, Malatya, Turkey

    Senem Arda Düz, Ercan Yılmaz, Ebru İnci Çoşkun, Erdinç Sarıdogan, Görkem Tuncay & Abdullah Karaer

  3. Laboratory of NMR, Scientific and Technological Research Center, Inonu University, Malatya, Turkey

    Akın Mumcu

  4. Department of Biomedical Engineering, School of Engineering, Inonu University, Malatya, Turkey

    Berat Doğan

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  1. Senem Arda Düz
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  2. Akın Mumcu
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  4. Ercan Yılmaz
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  8. Abdullah Karaer
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Contributions

SAD: protocol/project development, data collection, data analysis, and manuscript writing/editing. AM, BD: protocol/project development, data analysis, and manuscript writing/editing. EY, EİÇ : data collection. ES: data collection, manuscript writing/editing. GT: protocol/project development, manuscript writing/editing. AK: protocol/project development, data collection, data analysis, and manuscript writing/editing. All authors given final approval of the version to be published.

Corresponding author

Correspondence to Abdullah Karaer.

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The authors declare that they have no conflict of interest.

Ethical standards

Institutional review boards approved the study (Approval no: 19–119). All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki. Declaration and informed consent forms had been obtained from all study participants.

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Arda Düz, S., Mumcu, A., Doğan, B. et al. Metabolomic analysis of endometrial cancer by high-resolution magic angle spinning NMR spectroscopy. Arch Gynecol Obstet 306, 2155–2166 (2022). https://doi.org/10.1007/s00404-022-06587-0

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