Abstract
Purpose
To investigate the effect of silencing the YKL-40 gene on the expression of inflammatory factors and the effect of silencing the YKL-40 gene of THP-1 cells on endometrial cancer.
Methods
We used a siRNA targeting a sequence in YKL-40 (si-YKL-40) to transfect HEC-1A and THP-1 cells. Quantitative real-time polymerase chain reaction assay was performed to investigate the mRNA levels of YKL-40, IL-8 and MMP-9 in HEC-1A and THP-1 cells. Migration, and invasion assays were performed to identify the effects of co-culture with THP-1 cells that silenced YKL-40 gene on the migration and invasion capacity of HEC-1A cells. Tube formation ability were detected by Matrigel-based angiogenesis assay.
Results
We successfully transfected HEC-1A and THP-1 cells with lentivirus to silence the YKL-40 gene. Compared with the blank control group and NC group, the expression of YKL-40, IL-8 and MMP-9 which were examined by qRT-PCR in YKL-40-siRNA group was significantly reduced in the two cell lines; after co-cultured with the supernatant of transfected THP-1 cells, the migration and invasion ability of HEC-1A cells in YKL-40-siRNA group was significantly reduced; the number of tubes in the YKL-40-siRNA group was significantly reduced, the spacing between the tubes was significantly increased, and the structure of tubes was incomplete.
Conclusion
Silencing the YKL-40 gene in THP-1 cells can inhibit the expression of inflammatory factors, the invasion and migration of human endometrial cancer cells and the capacity of vitro angiogenic. And YKL-40 gene as a marker of inflammation may be an effective therapeutic target for endometrial cancer.
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Funding
The study was funded by the National Natural Science Foundation of China (No.81360388) and Guangxi Natural Science Foundation (No.2016 GXNSFAA380258).
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All the authors have read the manuscript and agreed to its being submitted for publication. All listed authors meet the appropriate authorship criteria. Nobody who qualifies for authorship has been omitted from the list. All authors have approved the acknowledgment of their contribution. LQ experimental operation, data collection or management, data analysis, manuscript writing/editing. FJT protocol development, data analysis. LL conceived the study, protocol development.
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Luo, Q., Fan, J. & Li, L. Silencing YKL-40 gene can inhibit inflammatory factor expression and affects the effect of THP-1 cells on endometrial cancer. Arch Gynecol Obstet 305, 467–473 (2022). https://doi.org/10.1007/s00404-021-06194-5
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DOI: https://doi.org/10.1007/s00404-021-06194-5