Skip to main content
SpringerLink
Log in

Isolation and identification of triterpenes from Anthemis austriaca Jacq. through bioactivity-guided fractionation on polycystic ovary syndrome rat model

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

This study aimed to assess the activity of A. austriaca flowers in treating polycystic ovary syndrome (PCOS) in rats.

Methods

A letrozole-induced PCOS rat model was used to evaluate the activity potential of A. austriaca flowers. For this purpose, extracts of different polarity were prepared from A. austriaca flowers using n-hexane, ethyl acetate, and methanol. Serum luteinizing hormone, follicle-stimulating hormone, progesterone, testosterone, estradiol, serum leptin, lipid, and glucose levels were tested. Moreover, the antioxidant activity was evaluated by calculating superoxide dismutase, malondialdehyde, catalase, and glutathione peroxidase levels. Following the biological activity studies, phytochemical studies were conducted on the active extract to detect the compound(s) responsible for the activity.

Results

The treatment with n-hexane extract contributed to regulating serum gonadotropin and steroid hormone levels. The plasma level of high-density lipoprotein cholesterol was significantly higher than that of the control group, while the levels of low-density lipoprotein cholesterol, leptin, and glucose were significantly lower than those of the control group. Also, the n-hexane extract showed significant antioxidant activity in the PCOS rat model. Since the n-hexane extract was found to be active, isolation studies were performed on this extract and three main fractions were obtained from the n-hexane extract. Those fractions also were tested on letrozole-induced PCOS rat model. As a result, three triterpenoids, β-amyrin palmitate, taraxasterol acetate, and taraxasterol were isolated and identified from Fr. B which is the most active fraction.

Conclusion

n-Hexane extract and Fr. B obtained from this extract showed statistically significant activity in the letrozole-induced PCOS rat model and three triterpene-type compounds were isolated from Fr. B.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Grierson AJC, Yavin Z (1975) Anthemis L. In: Davis PH (ed) Flora of Turkey and the East Aegean Islands. University of Edinburgh Press, Edinburgh, pp 174–221

    Google Scholar 

  2. Fujita T, Sezik E, Tabata M, Yeşilada E, Honda G, Takeda Y (1995) Traditional medicine in Turkey VII. Folk medicine in Middle and West Black Sea regions. Econ Bot 49(4):406–422

    Article  Google Scholar 

  3. Honda G, Yesilada E, Tabata M, Sezik E, Fujita T, Takeda Y, Takaishi Y, Tanaka T (1996) Traditional medicine in Turkey. VI. Folk medicine in West Anatolia: Afyon, Kütahya, Denizli, Muğla. Aydin provinces J Ethnopharmacol 53:75–87

    CAS  PubMed  Google Scholar 

  4. Simsek I, Aytekin F, Yesilada E, Yıldırımlı Ş (2004) An ethnobotanical survey of the Beypazari, Ayas, and Güdül district towns of Ankara province (Turkey). Econ Bot 58(4):705–720

    Article  Google Scholar 

  5. Kaval I, Behcet L, Cakilcioglu U (2014) Ethnobotanical study on medicinal plants in Gecitli and its surrounding (Hakkari-Turkey). J Ethnopharmacol 155(1):171–184

    Article  Google Scholar 

  6. Tetik F, Civelek S, Cakilcioglu U (2013) Traditional uses of some medicinal plants in Malatya (Turkey). J Ethnopharmacol 146(1):331–346

    Article  Google Scholar 

  7. Uysal I, Onar S, Karabacak E, Çelik S (2010) Ethnobotanical aspects of Kapıdağ Peninsula (Turkey). Biol Divers Conserv 3(3):15–22

    Google Scholar 

  8. Barbour EK, Al Sharif M, Sagherian VK, Habre AN, Talhouk RS, Talhouk SN (2004) Screening of selected indigenous plants of Lebanon for antimicrobial activity. J Ethnopharmacol 93(1):1–7

    Article  Google Scholar 

  9. Shahat AA, Ibrahim AY, Elsaid MS (2014) Polyphenolic content and antioxidant activity of some wild Saudi Arabian Asteraceae plants. Asian Pac J Trop Med 7(7):545–551

    Article  CAS  Google Scholar 

  10. Staneva J, Trendafilova-Savkova A, Todorova MN, Evstatieva L, Vitkova A (2004) Terpenoids from Anthemis austriaca Jacq. Z Naturforsch C 59(3–4):161–165

    Article  CAS  Google Scholar 

  11. Kovanci E, Buster JE (2006) Polycystic ovary syndrome. In: Bieber EJ, Sanfilippo JS, Horowitz IR (eds) Clinical Gynecology. Churchill Livingstone, Philadelphia, PA, USA, pp 893–909

    Google Scholar 

  12. Kahn JA (2008) Polycystic ovary syndrome. In: Slap GB (ed) Adolesc Med. Mosby, Philadelphia, PA, USA, pp 165–174

    Chapter  Google Scholar 

  13. Oyelowo T (2007) Polycystic ovary syndrome. In: Oyelowo T (ed) Mosby's Guide to Women's Health, Mosby, St. Louis, Missouri, USA, pp 176–179

    Chapter  Google Scholar 

  14. Rezvanfar MA, Rezvanfar MA, Ahmadi A, Shojaei-Saadi HA, Baeeri M, Abdollahi M (2012) Molecular mechanisms of a novel selenium-based complementary medicine which confers protection against hyperandrogenism-induced polycystic ovary. Theriogenology 78(3):620–631

    Article  CAS  Google Scholar 

  15. Rezvanfar MA, Ahmadi A, Saadi HS, Baeeri M, Abdollahi M (2012) Mechanistic links between oxidative/nitrosative stress and tumor necrosis factor alpha in letrozole-induced murine polycystic ovary: biochemical and pathological evidences for beneficial effect of pioglitazone. Hum Exp Toxicol 31(9):887–897

    Article  CAS  Google Scholar 

  16. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126

    Article  CAS  Google Scholar 

  17. Yagi K (1984) Assay for blood plasma or serum lipid peroxides methods. Methods Enzymol 105:328–331

    Article  CAS  Google Scholar 

  18. Fisher CR, Graves KH, Parlow AF, Simpson ER (1998) Characterization of mice deficient in aromatase (ArKO) because of targeted disruption of the cyp19 gene. Proc Natl Acad Sci 95:6965–6970

    Article  CAS  Google Scholar 

  19. Kafali H, Iriadam M, Ozardali I, Demir N (2004) Letrozole-induced polycystic ovaries in the rat: a new model for cystic ovarian disease. Arch Med Res 35:103–108

    Article  CAS  Google Scholar 

  20. Reddy PS, Begum N, Mutha S, Bakshi V (2016) Beneficial effect of curcumin in letrozole induced polycystic ovary syndrome. Asian Pac J Reprod 5(2):116–122

    Article  Google Scholar 

  21. Walters KA, Allan CM, Handelsman DJ (2012) Rodent models for human polycystic ovary syndrome. Biol Reprod 86(5):1–12

    Article  Google Scholar 

  22. Khaled N, El-Bahy AA, Radwan R, Handoussa H, Abdel Maksoud S (2019) Ocimum kilimandscharicum L. restores ovarian functions in letrozole-induced polycystic ovary syndrome (PCOS) in rats: Comparison with metformin. Life Sci 232:116640

    Article  CAS  Google Scholar 

  23. Ndeingang EC, Defo Deeh PB, Watcho P, Kamanyi A (2019) Phyllanthus muellerianus (Euphorbiaceae) restores ovarian functions in letrozole-induced polycystic ovarian syndrome in rats. Evid Based Complement Alternat Med 2019:2965821

    Article  Google Scholar 

  24. Yang H, Lee SY, Lee SR, Pyun BJ, Kim HJ, Lee YH, Kwon SW, Suh DH, Lee CH, Hong EJ, Lee HW (2018) Therapeutic effect of Ecklonia cava extract in letrozole-induced polycystic ovary syndrome rats. Front Pharmacol 9:1325

    Article  CAS  Google Scholar 

  25. Zawadski JK, Dunaif A (1992) The polycystic ovary syndrome. In: Dunaif A, Givens JR, Haseltine FP, Merriam GR (eds) The Polycystic Ovary Syndrome. Blackwell Scientific Publications, Boston, pp 377–384

    Google Scholar 

  26. Laven JS, Imani B, Eijkemans MJ, Fauser BC (2002) New approach to polycystic ovary syndrome and other forms of anovulatory infertility. Obstet Gynecol Surv 57(11):755–767

    Article  Google Scholar 

  27. Kaiser UB (2011) Gonadotropin Hormones. In: Melmed S (ed) The Pituitary, 3rd edn. Academic Press, New York, pp 205–260

    Chapter  Google Scholar 

  28. Marx TL, Mehta AE (2003) Polycystic ovary syndrome: pathogenesis and treatment over the short and long term. Cleve Clin J Med 70(1):31–45

    Article  Google Scholar 

  29. Burghen CA, Givens JR, Kitabchi AE (1980) Correlation of hyperandrogenism with hyperinsulinemia in polycystic ovarian disease. J Clin Endocrinol Metab 50:113–116

    Article  CAS  Google Scholar 

  30. Legro RS (2004) Polycystic ovarian syndrome. In: Leung PCK, Adashi EY (eds) The Ovary, 2nd edn. Academic Press, New York, pp 489–512

    Chapter  Google Scholar 

  31. Chang RJ (2009) Polycystic ovary syndrome and hyperandrogenic states. In: Strauss JF, Barbieri RL (eds) Yen & Jaffe's Reproductive Endocrinology, 6th edn. USA, W.B, Saunders, Philadelphia, PA, pp 489–516

    Chapter  Google Scholar 

  32. Zuo T, Zhu M, Xu W (2016) Roles of oxidative stress in polycystic ovary syndrome and cancers. Oxid Med Cell Longev 2016:8589318

    Article  Google Scholar 

  33. Verit FF, Erel O (2008) Oxidative stress in nonobese women with polycystic ovary syndrome: correlations with endocrine and screening parameters. Gynecol Obstet Invest 65(4):233–239

    Article  CAS  Google Scholar 

  34. Kucukkurt I, Ince S, Fidan AF, Ozdemir A (2008) The effects of dietary supplementation of different amount of Yucca schidigera powder (Sarsaponin 30®) on blood and tissue antioxidant defense systems and lipid peroxidation in rats. J Anim Vet Adv 7(11):1413–1417

    Google Scholar 

  35. Keles H, Ince S, Kucukkurt I, Tatli II, Akkol EK, Kahraman C, Demirel HH (2012) The effects of Feijoa sellowiana fruits on the antioxidant defense system, lipid peroxidation, and tissue morphology in rats. Pharm Biol 50(3):318–325

    Article  Google Scholar 

  36. Kyle ME, Miccadei S, Nakae D, Farber JL (1987) Superoxide dismutase and catalase protect cultured hepatocytes from the cytotoxicity of acetaminophen. Biochem Biophys Res Commun 149(3):889–896

    Article  CAS  Google Scholar 

  37. Matés JM, Sánchez-Jiménez F (1999) Antioxidant enzymes and their implications in pathophysiologic processes. Front Biosci 4:339–345

    Article  Google Scholar 

  38. Hsu C, Hsieh CL, Kuo YH, Huang CJ (2011) Isolation and identification of cucurbitane-type triterpenoids with partial agonist/antagonist potential for estrogen receptors from Momordica charantia. J Agric Food Chem 59(9):4553–4561

    Article  CAS  Google Scholar 

  39. Ruhlen RL, Sun GY, Sauter ER (2008) Black Cohosh: insights into its mechanism(s) of action. Integr Med Insights 3:21–32

    Article  CAS  Google Scholar 

  40. Sandjo LP, Kuete V (2013) Triterpenes and steroids from the medicinal plants of Africa. In: Kuete V (ed) Medicinal plant research in Africa: Pharmacology and Chemistry. Elsevier, London, UK, pp 135–202

    Chapter  Google Scholar 

Download references

Funding

This study was supported by 2214/A International Research Fellowship Program, provided by "The Scientific and Technological Research Council of Turkey (TUBITAK)".

Author information

Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmacy, Van Yüzüncü Yıl University, 65080, Tuşba, Van, Turkey

    Mert Ilhan

  2. National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA

    Mert Ilhan, Zulfiqar Ali & Ikhlas A. Khan

  3. Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, 06330, Ankara, Turkey

    Mert Ilhan & Esra Küpeli Akkol

  4. Department of Biology, Faculty of Science, Gazi University, Etiler, 06330, Ankara, Turkey

    Hakkı Tastan

Authors
  1. Mert Ilhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zulfiqar Ali
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ikhlas A. Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hakkı Tastan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Esra Küpeli Akkol
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

MI Isolation studies and Biological Activity Studies. ZA Isolation Studies and Structure Elucidation. IAK Isolation Studies and Structure Elucidation. HT Histopathological analysis. EKA Biological Activity Studies.

Corresponding author

Correspondence to Mert Ilhan.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ilhan, M., Ali, Z., Khan, I.A. et al. Isolation and identification of triterpenes from Anthemis austriaca Jacq. through bioactivity-guided fractionation on polycystic ovary syndrome rat model. Arch Gynecol Obstet 301, 1103–1111 (2020). https://doi.org/10.1007/s00404-020-05493-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00404-020-05493-7

Keywords

Search

Navigation