Abstract
Purpose
This study examined the relationship between the mechanism of hepatitis B virus (HBV) father–infant transmission via reproductive cells and pregnancy outcome.
Methods
Abandoned in vitro fertilization (IVF) embryos of fathers with chronic HBV infection were taken as study objects. HBV mRNA in embryos was detected, and successfully transplanted embryos were followed up to determine the relationship between HBV-infected embryos and pregnancy outcome.
Results
HBV mRNA signals were detected in one embryo in the group with HBV-positive fathers; the positive rate was 1/18 (5.5%). IVF embryos of HBV-positive fathers with HBV mRNA signals were successfully implanted, but early abortion occurred.
Conclusions
HBV mRNA was found in abandoned IVF embryos of HBV-infected fathers, which confirmed that HBV could not only enter early cleavage embryos via sperm but also replicate in embryos, resulting in HBV father–infant transmission. HBV may interfere with embryonic development and thus affect pregnancy outcome.
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References
Trépo C, Chan HL, Lok A (2014) Hepatitis B virus infection. Lancet 384:2053–2063
WHO Guidelines Approved by the Guidelines Review Committee (2015) Guidelines for the prevention, care and treatment of persons with chronic hepatitis B infection
Chinese Society of Hepatology and Chinese Society of Infectious Diseases, Chinese Medical Association (2011) The guideline of prevention and treatment for chronic hepatitis B (2010 version). Zhonghua Gan Zang Bing Za Zhi 19:13–24
Hadchouel M, Scotto J, Huret JL, Molinie C, Villa E, Degos F, Brechot C (1985) Presence of HBVDNA in spermatozoa: a possible vertical transmission of HBV via the germ line. J Med Virol 16:61–66
Davison F, Alexander GJ, Trowbridge R, Fagan EA, Williams R (1987) Detection of hepatitis B virus DNA in spermatozoa, urine, saliva and leucocytes, of chronic HBsAg carriers. A lack of relationship with serum markers of replication. J Hepatol 4:37–44
Naumova AK, Korenev VI, Leonov BV, Tsibinogin VV, Kiselev LL (1986) DNA of the hepatitis B virus in human generative cells. Genetika 22:166–168
Huang JM, Huang TH, Qiu HY, Fang XW, Zhuang TG, Qiu JW (2002) Studies on the integration of hepatitis B virus DNA sequence in human sperm chromosomes. Asian J Androl 4:209–212
Hu XL, Zhou XP, Qian YL, Wu GY, Ye YH, Zhu YM (2011) The presence and expression of the hepatitis B virus in human oocytes and embryos. Hum Reprod 26:1860–1867
Hayes CN, Akamatsu S, Tsuge M, Miki D, Akiyama R, Abe H, Ochi H, Hiraga N, Imamura M, Takahashi S, Aikata H, Kawaoka T, Kawakami Y, Ohishi W, Chayama K (2012) Hepatitis B virus-specific miRNAs and Argonaute2 play a role in the viral life cycle. PLoS One 7:e47490
Nakabayashi J (2016) The intracellular dynamics of hepatitis B virus (HBV) replication with reproduced virion “re-cycling”. J Theor Biol 396:154–162
Oger P, Yazbeck C, Gervais A, Dorphin B, Gout C, Jacquesson L, Ayel JP, Kahn V, Rougier N (2011) Adverse effects of hepatitis B virus on sperm motility and fertilization ability during IVF. Reprod Biomed Online 23:207–212
Chen JW, Cui Y, Zhang XX (2011) Investigate the impact of hepatitis B virus infection on sperm DNA integrity. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 25:345–347
Gao L, Liu YH, Li LF, Wu YM, Wang MB, Shi J, Yuan B, Song J, He YM, Wei DK (2009) Changes of peripheral blood chromosomal centromere aberration in patients with cytomegalovirus infection after anti-viral treatment. Nan Fang Yi Ke Da Xue Xue Bao 29:1846–1847
Putland RA, Ford J, Korban G, Evdokiou A, Tremaine M (1990) Investigation of spontaneously aborted concepti for microbial DNA: investigation for cytomegalovirus DNA using polymerase chain reaction. Aust N Z J Obstet Gynaecol 30:248–250
Zhou XP, Hu XL, Zhu YM, Qu F, Sun SJ, Qian YL (2011) Comparison of semen quality and outcome of assisted reproductive techniques in Chinese men with and without hepatitis B. Asian J Androl 13:465–469
Hu Y, Chen X, Chen LL, Xu ZF, Wang X, Cui H (2006) Comparative genomic hybridization analysis of spontaneous abortion. Int J Gynaecol Obstet 92:52–57
Shi L, Liu S, Zhao W, Zhou H, Ren W, Shi J (2014) Hepatit is B virus infection reduces fertilization ability during in vitro fertilization and embryo transfer. J Med Virol 86:1099–1104
Lee VC, Ng EH, Yeung WS, Ho PC (2010) Impact of positive hepatitis B surface antigen on the outcome of IVF treatment. Reprod Biomed Online 21:712–717
Lorusso F, Palmisano M, Chironna M, Vacca M, Masciandaro P, Bassi E, Selvaggi Luigi L, Depalo R (2010) Impact of chronic viral diseases on semen parameters. Andrologia 42:121–126
Shih WL, Yu MW, Chen PJ, Yeh SH, Lo MT, Chang HC, Liaw YF, Lin SM, Liu CJ, Lee SD, Lin CL, Hsiao CK, Yang SY, Chen CJ (2006) Localization of a susceptibility locus for hepatocellular carcinoma to chromosome 4q in a hepatitis B hyperendemic area. Oncogene 25:3219–3224
Moretti E, Federico MG, Giannerini V, Collodel G (2008) Sperm ultrastructure and meiotic segregation in a group of patients with chronic hepatitis B and C. Andrologia 40:286–291
Vicari E, Arcoria D, Di Mauro C, Noto R, Noto Z, La Vignera S (2006) Sperm output in patients with primary infertility and hepatitis B or C virus; negative influence of HBV infection during concomitant varicocele. Minerva Med 97:65–77
Chen H, Ge HS, Lv JQ, Wu XM, Xi HT, Huang JY, Zhu CF (2014) Chronic hepatitis B virus infection in women is not associated with IVF/ICSI outcomes. Arch Gynecol Obstet 289:213–217
Acknowledgements
This study was supported by National Natural Science Foundation of China (No. 30371277).
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This study was conducted in accordance with the declaration of Helsinki. This study was conducted with approval from the Ethics Committee of The First Affiliated Hospital of Xi’an Jiaotong University. Written informed consent was obtained from all participants.
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We declare that we have no conflict of interest.
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Kong, Y., Liu, Y., Liu, X. et al. Relationship between the mechanism of hepatitis B virus father–infant transmission and pregnancy outcome. Arch Gynecol Obstet 295, 253–257 (2017). https://doi.org/10.1007/s00404-016-4231-6
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DOI: https://doi.org/10.1007/s00404-016-4231-6