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Evaluation of the frequency of G-765C polymorphism in the promoter region of the COX-2 gene and its correlation with the expression of this gene in the endometrium of women with endometriosis

  • General Gynecology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Objective

To evaluate the frequency of polymorphism G-765C (rs20417) of the COX-2 gene and the expression of this gene in the endometrium of women with endometriosis.

Study design

This is a case–control study of 365 women with endometriosis (251 infertile and 114 fertile) submitted to laparoscopy/laparotomy with histological confirmation of endometriosis. The control group was composed of 522 fertile women without endometriosis. Of these, 37 patients from the endometriosis group and 47 from the control group were submitted to biopsy of the endometrium for analysis of the expression of the COX-2 gene. The genotypes were determined using analysis by High-Resolution Melt. Gene expression was measured by qRT-PCR with TaqMan methodology using the GAPDH gene as normalizer of the reactions.

Results

The distribution of the genotypes and alleles in the group of fertile women with moderate/severe endometriosis showed a statistically significant difference, demonstrating association of the ancestral allele, −765G, with increased risk of endometriosis (p = 0.028; OR 0.53; CI 0.32–0.90). The mean expression of the COX-2 gene (mRNA PTGS2) in the group of women with endometriosis was statistically higher compared to the control group (3.85 versus 2.84, p = 0.028).

Conclusion

The present study identified that in Brazilian women the presence of the ancestral allele, −765G, of the COX-2 gene is associated with an increased risk for development of moderate/severe endometriosis associated with fertility, and that the eutopic endometrium of women with endometriosis showed increased expression of COX-2 when compared to the control group.

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Acknowledgments

The authors wish to thank FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) for the financial aid of the project (2012/03564-0) and the scholarship to the student Tatiana Guida Ponce (2013/07978-6), and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for the scholarship to the student Viviane Maria Bezerra Cavalcanti Lins.

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Authors and Affiliations

  1. Department of Collective Health, Faculdade de Medicina do ABC, Human Reproduction and Genetics Center, Avenida Príncipe de Gales, 821, Santo André, SP, 09060-650, Brazil

    Viviane Cavalcanti, Tatiana Guida Ponce, Fernanda Abani Mafra, Gustavo Mendonça André, Denise Maria Christofolini, Caio Parente Barbosa & Bianca Bianco

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  1. Viviane Cavalcanti
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  2. Tatiana Guida Ponce
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  3. Fernanda Abani Mafra
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  4. Gustavo Mendonça André
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  5. Denise Maria Christofolini
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  6. Caio Parente Barbosa
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  7. Bianca Bianco
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Correspondence to Bianca Bianco.

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Cavalcanti, V., Ponce, T.G., Mafra, F.A. et al. Evaluation of the frequency of G-765C polymorphism in the promoter region of the COX-2 gene and its correlation with the expression of this gene in the endometrium of women with endometriosis. Arch Gynecol Obstet 293, 109–115 (2016). https://doi.org/10.1007/s00404-015-3808-9

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  • DOI: https://doi.org/10.1007/s00404-015-3808-9

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